Identification of TGFβ target genes in pancreatic cancer cells by cDNA representational difference analysis

Geng, Michael; Adler, Gail K.; Gress, T

doi:10.1016/s0016-5085(98)82447-5

Cited by 5 publications

(3 citation statements)

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“…The prevalent localization of ACF in the distal and middle colon has been reported by other researchers, who suggested that ACF are not random lesions of the rodent colon treated with genotoxic colon carcinogens (Geng, Adler, & Gress, 1998;Rodrigues, Silva, Salvadori, de Camargo, & Montenegro, 2002). Among the different treatment groups, the mice receiving 0.25% EGCG derivatives had smaller number of medium ACF (P7 AC/focus) in their distal colon.…”

Section: Inhibition Of Aom-induced Acf In Mouse Colonsupporting

confidence: 64%

Protective effects of epigallocatechin gallate (EGCG) derivatives on azoxymethane-induced colonic carcinogenesis in mice

Zhong

Chiou

Pan

et al. 2012

Journal of Functional Foods

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Colon cancerEGCG ester iNOS COX-2 A B S T R A C T Epigallocatechin gallate (EGCG), the major polyphenol in green tea and a functional food ingredient/nutraceutical with health-promoting properties, was structurally modified by esterification with butyric and docosahexaenoic (DHA) acid in order to improve its lipophilicity and hence bioefficacy in vivo. The lipophilic derivatives of EGCG so-prepared were evaluated for their anticancer activity against azoxymethane (AOM)-induced colon carcinogenesis in mice. Formation of colonic aberrant crypt foci (ACF) was monitored as the biomarker of colorectal cancer (CRC). It was found that oral administration of EGCG derivatives led to reduced size of ACF in the mouse colon. EGCG-DHA esters were more effective than EGCG-butyrate in inhibiting the formation of ACF. The total number of large colonic ACF was remarkably decreased by treatment with EGCG derivatives, especially by the EGCG-DHA esters, which showed a 100% inhibition of large ACF formation. Two tumor-promoting enzymes, iNOS and COX-2 were also inhibited by EGCG derivatives to various extents at the expression level. The results suggest that the lipophilic ester derivatives of EGCG are effective in inhibiting colon carcinogenesis and may be good candidates for colon cancer prevention/treatment.

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Section: Inhibition Of Aom-induced Acf In Mouse Colonsupporting

confidence: 64%

Protective effects of epigallocatechin gallate (EGCG) derivatives on azoxymethane-induced colonic carcinogenesis in mice

Zhong

Chiou

Pan

et al. 2012

Journal of Functional Foods

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“…To analyze the effect of TGF-b1 on cell morphology pancreatic cancer cell lines were grown to 70% confluence in DMEM containing 10% FCS. Afterwards, cells were washed twice in serum free medium, starved for 24 h in serum free medium and finally treated for 12 and 24 h with 10 ng ml 71 TGF-b1 (R&D, Wiesbaden, Germany) or medium alone (Geng et al, 1999). Thereafter total RNA was extracted using the RNAclean kit (AGS, Heidelberg, Germany) as indicated by the manufacturer.…”

Section: Cell Linementioning

confidence: 99%

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

et al. 2002

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PTEN is a candidate tumour suppressor gene and frequently mutated in multiple cancers, however, not in pancreatic cancer.Recently, it has been demonstrated that PTEN expression is regulated by TGF-b1. Using TGF-b1 transgenic mice (n=7) and wildtype littermates (n=6), as well as pancreatic tissues obtained from organ donors (n=10) and patients with pancreatic cancer (n=10), we assessed the expression of PTEN by means of immunohistochemistry and semiquantitative PCR analysis. In addition, PANC-1 cells were treated with TGF-b1 in vitro and the levels of PTEN mRNA were determined in these cells. In human pancreatic cancers PTEN mRNA levels were significantly decreased (P50.05). In addition, in the pancreas of TGF-b1 transgenic mice the expression of PTEN was significantly reduced (P50.01), as compared to wildtype littermates and incubation of PANC-1 cells with TGF-b1 decreased PTEN mRNA levels after 24 h. Inasmuch as TGF-b1 decreases PTEN expression in human pancreatic cancer cells and human pancreatic cancers overexpress TGF-b1, the reduced expression of PTEN in pancreatic cancer may be mediated by TGF-b1 overexpression. Thus, although PTEN is not mutated in pancreatic cancers, the reduction of its expression may give pancreatic cancer cells an additional growth advantage.

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“…In this context we developed a serial characterization approach allowing us to identifiy genes associated, e.g., with the invasive/metastatic potential of pancreatic cancer cells, embryonal development or transcriptionally regulated by growth factors as epidermal growth factor (EGF) or transforming growth factor-β (TGF-β). 9 In this serial characterization approach all cDNA clones classified as differentially expressed were spotted in duplicate onto 9 × 13 cm nylon membranes to facilitate automated analysis. To identifiy, e.g., TGF-β or EGF target genes these small sublibraries are hybridized with cDNA probes from cell lines treated with the respective growth factor or from untreated cells.…”

Section: Expression Profiling Using Automated Cdna Library Technologymentioning

confidence: 99%

Strategies for the Detection of Disease Genes in Pancreatic Cancer

Wallrarr

Müller-Pillasch

Micha

et al. 1999

Annals of the New York Academy of Sciences

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The present review summarizes our strategies aimed at identifying and characterizing genetic alterations occurring at the transcriptional and chromosomal level in pancreatic cancer. To study transcriptional alterations we have used a number of techniques including modified versions of differential hybridizations and cDNA RDA (representational difference analysis). These approaches have led to the identification of more than 500 genes with differential expression in pancreatic cancer. To study chromosomal aberrations occurring in pancreatic cancer tissues we used comparative genomic hybridization (CGH). This allowed the identification of a number of chromosomal regions containing putative tumor suppressor genes or oncogenes. Genes isolated in both approaches represent potential new disease genes for pancreatic cancer and are at present being characterized by individual or serial analysis.

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Identification of TGFβ target genes in pancreatic cancer cells by cDNA representational difference analysis

Cited by 5 publications

References 0 publications

Protective effects of epigallocatechin gallate (EGCG) derivatives on azoxymethane-induced colonic carcinogenesis in mice

Protective effects of epigallocatechin gallate (EGCG) derivatives on azoxymethane-induced colonic carcinogenesis in mice

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

Strategies for the Detection of Disease Genes in Pancreatic Cancer

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