Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling

Kang, Igojo; Choi, Yourim; Jung, Sueun; Lim, Jae Yun; Lee, Dong Hoon; Gupta, Sumeet; Moon, Woongjoon; Shin, Chanseok

doi:10.1038/s41598-018-25483-9

Cited by 7 publications

(9 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Loss of function of the components of the heterochromatin machinery and of the piRNA pathway induce apoptotic GSC loss accompanied with transposon derepression, DNA damage and checkpoint activation (Chen et al, 2007;Klattenhoff et al, 2007;Ma et al, 2014Ma et al, , 2017. In egg mutants, upregulation of the apoptotic genes hid and reaper, and activation of Caspase 3 cleavage was found (Clough et al, 2007;Kang et al, 2018). Similarly, we observed an accumulation of DNA damage, checkpoint activation and apoptosis in sov-depleted cells.…”

Section: Sov Functions In the Stem Cell Nichesupporting

confidence: 55%

“…Another level of transposon regulation, where Sov is involved, is the indirect control of piRNA biogenesis. Like egg, sov is required for Ago3 transcription, raising the possibility that the transposon derepression defects found in sov-depleted germ cells may result not only from reduced cluster transcription, but also from defective piRNA biogenesis (Kang et al, 2018). However, the effect of sov on transposon repression and niche regulation differs from that of Ago3.…”

Section: Sov Is Involved In Transposon Repressionmentioning

confidence: 99%

See 1 more Smart Citation

Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation

et al. 2018

View full text Add to dashboard Cite

Self-renewal and differentiation of stem cells is one of the fundamental biological phenomena relying on proper chromatin organization. In our study, we describe a novel chromatin regulator encoded by the Drosophila small ovary (sov) gene. We demonstrate that sov is required in both the germline stem cells (GSCs) and the surrounding somatic niche cells to ensure GSC survival and differentiation. sov maintains niche integrity and function by repressing transposon mobility, not only in the germline, but also in the soma. Protein interactome analysis of Sov revealed an interaction between Sov and HP1a. In the germ cell nuclei, Sov colocalizes with HP1a, suggesting that Sov affects transposon repression as a component of the heterochromatin. In a position-effect variegation assay, we found a dominant genetic interaction between sov and HP1a, indicating their functional cooperation in promoting the spread of heterochromatin. An in vivo tethering assay and FRAP analysis revealed that Sov enhances heterochromatin formation by supporting the recruitment of HP1a to the chromatin. We propose a model in which sov maintains GSC niche integrity by regulating transposon silencing and heterochromatin formation.

show abstract

Section: Sov Functions In the Stem Cell Nichesupporting

confidence: 55%

Section: Sov Is Involved In Transposon Repressionmentioning

confidence: 99%

Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…(Ma et al, 2014;Ma et al, 2017). In egg mutants, upregulation of apoptotic genes hid, reaper, and activation of Caspase3 cleavage was found (Clough et al, 2007;Kang et al, 2018).…”

Section: Sov Functions In the Stem Cell Nichementioning

confidence: 95%

“…Another level of transposon regulation, where Sov is involved, is the indirect control of piRNA biogenesis. Like egg, sov is required for Ago3 transcription, raising the possibility that the transposon derepression defects found in sov depleted germ cells may result not only from reduced cluster transcription, but also from defective piRNA biogenesis (Kang et al, 2018).…”

Section: Sov Is Involved In the Pirna Pathwaymentioning

confidence: 99%

Drosophilasmall ovarygene ensures germline stem cell maintenance and differentiation by silencing transposons and organising heterochromatin

Jankovics

Bence

Sinka

et al. 2018

Preprint

View full text Add to dashboard Cite

Summary statement:Small ovary maintains the integrity of the stem cell niche by regulating piRNA-mediated transposon silencing acting as a key component of the heterochromatin.All rights reserved. No reuse allowed without permission.was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/383265 doi: bioRxiv preprint first posted online Aug. 2, 2018; 2 AbstractSelf-renewal and differentiation of stem cells is one of the fundamental biological phenomena relying on proper chromatin organisation. In our study, we describe a novel chromatin regulator encoded by the Drosophila small ovary (sov) gene. We demonstrate that sov is was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

show abstract

“…The Drosophila eggless/dSetdb1 protein is responsible for trimethylation of H3K9 and is essential for viability and fertility [39,40]. It is required at multiple stages of oogenesis, maintenance and differentiation of Germline and Follicle Stem Cells.…”

Section: Retrieval Of Curated Methylases and Demethylases Orthoclustersmentioning

confidence: 99%

A comparative analysis of histone methyltransferases and demethylases in insect genome: A meta-analysis

Gulati

Kohli

Narang

et al. 2019

Preprint

View full text Add to dashboard Cite

Abbreviations: KMT-lysine methyltransferases, PRMT-Protein arginine methyltransferases, PTM-post-translational modification, DSB-double strand break Abstract Background: The epigenetic regulation through post-translational modification of histones, especially methylation is well conserved, while DNA methylation is variable, being very low or absent in Drosophila melanogaster. Though there are several insect genomes sequenced, an analysis with a focus on their epigenetic repertoire is limited. We have compared the histone methyltransferases and the demethylases in the genome of Drosophila melanogaster, Aedes aegypti (Diptera), the pea aphid Acyrthosiphon pisum, the triatomid bug Rhodnius prolixus (Hemiptera), the honeybee Apis mellifera (Hymenoptera), the silkworm Bombyx mori (Lepidoptera) and the red flour beetle Tribolium castaneum (Coleoptera). Results:We identified 38 clusters consisting of arginine, lysine methyltransferases and demethylases using OrthoFinder. To eliminate false positives, we designed a method based on identifying highly conserved domain within each class designated as the high priority domain. Out of the 9 arginine methyltransferases, Art2, Art6 and Art9 are identified in D.melanogaster only. We observe copy number variation between the genomes; A.pisum has nine copies of eggless gene (H3K9me3 methyltransferase), which can be correlated with the switch between parthenogenesis and sexual reproduction. Other than the high-priority domains, these proteins contain shared and unique domains that can mediate protein-protein interaction. Phylogenetic analysis indicates that the there is a broad conservation within the members of a class while duplication and divergence is observed in LSD1. Conclusion:This meta-analysis provides a method for reliable identification of epigenetic modifiers of histones in newly sequenced insect genomes. Similar approach can be taken for other classes of genes.Drome: Drosophila melanogaster, Aedae: Aedes aegypti, Acypi: Acyrthosiphon pisum, Rhopr:Rhodnius prolixus, Apime: Apis mellifera, Bommo: Bombyx mori, Trica: Tribolium castaneum.Art9 is identified only in D. melanogaster and the two isoforms (99.2% identity), were grouped into two singleton clusters as additional 53 amino acids are present at the N-terminal in one of them. As

show abstract

Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling

Cited by 7 publications

References 38 publications

Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation

Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation

Drosophilasmall ovarygene ensures germline stem cell maintenance and differentiation by silencing transposons and organising heterochromatin

A comparative analysis of histone methyltransferases and demethylases in insect genome: A meta-analysis

Contact Info

Product

Resources

About