Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

Yoon, June‐Sun; Sahoo, Dipak Kumar; Maiti, Indu B.; Palli, Subba Reddy

doi:10.1038/s41598-018-32742-2

Cited by 25 publications

(28 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study where T. urticae was fed on Arabidopsis leaves coated with in vitro-transcribed dsRNA targeting the vacuolar ATPase, the mite exhibited visible dark body phenotype but lethality was not observed (Suzuki et al, 2017). Interestingly, to identify the most suitable RNAi target genes for mite mortality, two independent studies suggested that coatamer I genes and genes involved in the biosynthesis and action of juvenile hormone and action of ecdysteroids were the best candidates (Kwon et al, 2016;Yoon et al, 2018) and should be thus taken into consideration for future RNA applications.…”

Section: Applying Rna Molecules In Plants Against Mitesmentioning

confidence: 99%

Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants

et al. 2019

View full text Add to dashboard Cite

show abstract

Section: Applying Rna Molecules In Plants Against Mitesmentioning

confidence: 99%

Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Expression of T. evansi Vicosa-1 or T. urticae Santpoort-2 genes by means of RT-qPCR is often normalized using housekeeping gene RP49 (Morales et al, 2016;Villarroel et al, 2016;Schimmel et al, 2017a;Suzuki et al, 2017;Jonckheere et al, 2018;Yoon et al, 2018). However, Yang et al (2015) warned that expression of RP49 and other housekeeping genes may not be suitable for normalizing gene expression levels across developmental stages.…”

Section: Expression Of Housekeeping Gene Rp49 Is Variable Across Spidmentioning

confidence: 99%

Juvenile Spider Mites Induce Salicylate Defenses, but Not Jasmonate Defenses, Unlike Adults

et al. 2020

View full text Add to dashboard Cite

When plants detect herbivores they strengthen their defenses. As a consequence, some herbivores evolved the means to suppress these defenses. Research on induction and suppression of plant defenses usually makes use of particular life stages of herbivores. Yet many herbivorous arthropods go through development cycles in which their successive stages have different characteristics and lifestyles. Here we investigated the interaction between tomato defenses and different herbivore developmental stages using two herbivorous spider mites, i.e., Tetranychus urticae of which the adult females induce defenses and T. evansi of which the adult females suppress defenses in Solanum lycopersicum (tomato). First, we monitored egg-to-adult developmental time on tomato wild type (WT) and the mutant defenseless-1 (def-1, unable to produce jasmonate-(JA)defenses). Then we assessed expression of salivary effector genes (effector 28, 84, SHOT2b, and SHOT3b) in the consecutive spider mite life stages as well as adult males and females. Finally, we assessed the extent to which tomato plants upregulate JA-and salicylate-(SA)-defenses in response to the consecutive mite developmental stages and to the two sexes. The consecutive juvenile mite stages did not induce JA defenses and, accordingly, egg-to-adult development on WT and def-1 did not differ for either mite species. Their eggs however appeared to suppress the SA-response. In contrast, all the consecutive feeding stages upregulated SA-defenses with the strongest induction by T. urticae larvae. Expression of effector genes was higher in the later developmental stages. Comparing expression in adult males and females revealed a striking pattern: while expression of effector 84 and SHOT3b was higher in T. urticae females than in males, this was the opposite for T. evansi. We also observed T. urticae females to upregulate tomato defenses, while T. evansi females did not. In addition, of both species also the males did not upregulate defenses. Hence, we argue that mite ontogenetic niche shifts and stagespecific composition of salivary secreted proteins probably together determine the course and efficiency of induced tomato defenses.

show abstract

“…In our study, feeding dsTuCPR optimize the efficiency of RNAi (Fig. 5A) [41,42,71,72]. Downregulation of TuCPR increased 447 susceptibilities of T. urticae populations to multiple acaricides, including abamectin, bifenthrin 448 and fenpyroximate, which are all commonly used for T. urticae control (Fig.…”

mentioning

confidence: 71%

RNA interference of NADPH-Cytochrome P450 reductase increases susceptibilities to multiple acaricides inTetranychus urticae

Adesanya

Cárdenas

Walsh

et al. 2019

Preprint

View full text Add to dashboard Cite

The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of acaricides, which leads to pervasive development of acaricide resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of acaricide resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in acaricide resistance in urticae. The full-length cDNA sequence of T. urticae’s CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of resistance to multiple acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.HighlightsPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate resistance in T. urticae populationsT. urticae’s cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzedAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionSilencing of TuCPR in T. urticae caused a reduction in acaricide resistance

show abstract

Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

Cited by 25 publications

References 31 publications

Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants

Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants

Juvenile Spider Mites Induce Salicylate Defenses, but Not Jasmonate Defenses, Unlike Adults

RNA interference of NADPH-Cytochrome P450 reductase increases susceptibilities to multiple acaricides inTetranychus urticae

Contact Info

Product

Resources

About