Identification of T-cell subpopulations in granuloma annulare

Abstract

Granuloma annulare is a lymphohistiocytic process of unknown cause characterized by necrobiotic dermal papules. Acetone-fixed frozen sections of eight granuloma annulare lesions were studied for the presence of T-cell subsets, using monoclonal antibodies to T-cell surface antigens. The palisading and perivascular lymphocytic infiltrate shows that most of the mononuclear cells are reactive with LEU-1 antibody, which characterizes peripheral T cells and activated T cells. Numerous cells were reactive with LEU-3a antibody, which identifies the helper-inducer T-cell subset. Some cells were positive with LEU-2a antibody, which detects suppressor-cytotoxic cell populations. Masses of histiocytes were identified infiltrating between the collagen fibers and surrounding the central necrobiosis area; the histiocytes showed a very strong diffuse acid phosphatase and nonspecific esterase activity. These findings suggest that a cell-mediated immune response may be the dominant pathogenic event in granuloma annulare.

T-Zone Histiocytes in Granulomatous Skin Diseases

“…Buechner et al [16] found numerous T-hclper cells in gran uloma annulare, which is also confirmed by our results obtained with monoclonal anti bodies OKT 11 and Leu 3a on frozen sec tions. Quismorio et al [17] and M is lira et al [18] demonstrated considerable numbers of T-cells in sarcoidosis lesions and Kveim reactions.…”

T-Cell Subsets in Acne rosacea Lesions and the Possible Role of <i>Demodex folliculorum</i>

“…[10], The detection of hybridoma binding to these cells was obtained by peroxidase-conjugated goal anti mouse IgCi utilizing a peroxidase histochemical reac tion.…”

Granuloma anulare giganteum et disseminatum

“…Proske U, Wozel G. Granuloma anulare giganteum et disseminatum … Akt Dermatol 2011; 37: 210 -213 nehmlich T-Helferzellen, unterstützt die Hypothese einer zellvermittelten Immunreaktion vom Spättyp auf ein unbekanntes Antigen [7]. Zur Behandlung des Granuloma anulare stehen verschiedene therapeutische Optionen mit unterschiedlichen Erfolgen zur Verfügung.…”