Identification of SWI2/SNF2-Related 1 Chromatin Remodeling Complex (SWR1-C) Subunits in Pineapple and the Role of Pineapple SWR1 COMPLEX 6 (AcSWC6) in Biotic and Abiotic Stress Response

Abstract

Chromatin remodeling complex orchestrates numerous aspects of growth and development in eukaryotes. SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is a member of the SWI/SNF ATPase-containing chromatin remodeling complex responsible for the exchange of H2A for H2A.Z. In plants, SWR1-C plays a crucial role by transcriptionally regulating numerous biological and developmental processes. However, SWR1-C activity remains obscure in pineapple. Here, we aim to identify the SWR1-C subunits in pineapple. By genome-wide identification, we found a total of 11 SWR1-C subunits in the pineapple. The identified SWR1-C subunits were named and classified based on the sequence similarity and phylogenetic analysis. RNA-Seq analysis showed that pineapple SWR1-C subunits are expressed differentially in different organs and at different stages. Additionally, the qRT-PCR of pineapple SWR1-C subunits during abiotic stress exposure showed significant changes in their expression. We further investigated the functions of pineapple SWR1 COMPLEX 6 (AcSWC6) by ectopically expressing it in Arabidopsis. Interestingly, transgenic plants ectopically expressing AcSWC6 showed susceptibility to fungal infection and enhanced resistance to salt and osmotic stress, revealing its involvement in biotic and abiotic stress. Moreover, the complementation of mutant Arabidopsis swc6 by pineapple SWC6 suggested the conserved function of SWC6 in plants.

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

“…One of the biggest issues concerning the impact of climate change on human being and natural ecosystems is the reaction of the Earth's hydrologic cycle to global warming [1,2]. As sessile species,…”

Comparative Transcriptome Analysis on the Regulatory Mechanism of Thoracic Ganglia in Eriocheir sinensis at Post-Molt and Inter-Molt Stages

“…EPHA2 functions in the regulation of brain development and angiogenesis [ 33 ]. ISWI plays a pivotal regulatory part in larval blood cell development and metamorphosis [ 34 ]. YAP1 , a transcriptional regulator, plays a critical role in the regulation of tissue tension and shape [ 35 ].…”

Identification and expression analysis of pineapple sugar transporters reveal their role in the development and environmental response

“…The cDNA was then used for RT-qPCR with 2X qPCR superMix (TransGen) in a 20 μL reaction on a Bio-Rad CFX96 Touch™ real-time PCR machine (Bio-Rad, Singapore). The cycling parameters were: 95°C for 30 s; 40 cycles of 95°C for 10 s and 60°C for 15 s. The fold change in the gene expression was determined using the EF1 α gene as the internal control using Livak method (2 −ΔΔCT ) as described earlier ( Livak and Schmittgen, 2001 ; Aslam et al., 2019 ; Jakada et al., 2019 ; Aslam et al., 2020 ). Three biological replicates and at least three independent technical replicates were performed in each condition.…”