Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells

Schwab, Kjiana Elkje; Hutchinson, Paul; Gargett, Caroline E.

doi:10.1093/humrep/den051

Cited by 182 publications

(132 citation statements)

References 52 publications

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“…More recently, they were also found in the human uterus endometrium and in menstrual blood and have been shown capable of promoting regeneration in vivo [16,18,19,21,22,32,33]. A recent study demonstrated isolating stem cells from the endometrium and promoting in vitro chondrogenesis [20].…”

Section: Discussionmentioning

confidence: 99%

“…The extraordinary regenerative capacity of the human endometrium following menstruation, in the postpartum period, after surgical procedures (uterine curettage, endometrial ablation) and in postmenopausal women undergoing hormonal replacement therapy suggests that MSC niches present in this tissue could be responsible for this process [16]. Indeed, endometrial and menstrual blood-derived stem cells were recently isolated and showed the ability to differentiate into cell types of the three germ layers [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures

Jazedje

Perin

Czeresnia³

et al. 2009

J Transl Med

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Background: The possibility of using stem cells for regenerative medicine has opened a new field of investigation. The search for sources to obtain multipotent stem cells from discarded tissues or through non-invasive procedures is of great interest. It has been shown that mesenchymal stem cells (MSCs) obtained from umbilical cords, dental pulp and adipose tissue, which are all biological discards, are able to differentiate into muscle, fat, bone and cartilage cell lineages. The aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from human fallopian tubes (hFTs).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures

Jazedje

Perin

Czeresnia³

et al. 2009

J Transl Med

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“…Several markers for stem cells in human uterus have been identified; however, these markers are not highly specific for endometrial stem cells (Schwab et al 2008). In the endometrium, the most commonly mentioned markers, indicating stem cell properties are cell membrane antigen C-KIT, also known as CD117, and intracellular transcription factor OCT3/4.…”

Section: Introductionmentioning

confidence: 99%

“…In the endometrium, the most commonly mentioned markers, indicating stem cell properties are cell membrane antigen C-KIT, also known as CD117, and intracellular transcription factor OCT3/4. The C-KIT marker is usually associated with hematopoietic stem cells and its expression may suggest a medullary origin of these stem cells (Cho et al 2004, Schwab et al 2008. Moreover, identification of two other nuclear transcription factors, NANOG and SOX2, allows for determining the presence of stem cells in the uterus.…”

Section: Introductionmentioning

confidence: 99%

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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“…Somatic stem cells (SCs) are a subset of cells residing in normal adult tissues that, through asymmetric division, retain their ability to self-renew while producing daughter cells that go on to differentiate and play a role in regeneration and repair of the native host tissue [Ono et al 2012;Schwab et al 2008]. Thus their differentiation capacities are usually restricted to the tissue's derivatives of particular germ layer of their origin.…”

Section: Introductionmentioning

confidence: 99%

Comparative systems genetics view of endometriosis and uterine leiomyoma: Two sides of the same coin?

Baranov

Ivaschenko

Yarmolinskaya

2016

Systems Biology in Reproductive Medicine

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Endometriosis (EM) and uterine leiomyoma (UL) are two most frequent benign tumors of monoclonal origin affecting about 30% of all women in their reproductive age. Modern molecular technologies have made a tremendous impact in understanding both disorders. Here is the first comparative analysis of molecular mechanisms underlying development of EM and UL as it looks from the platform of systems genetics. Similarities and differences of EM and UL at their incipient stages are enlightened with special emphasis on their gene networks, gene expression, and epigenetic regulation, of pathologic development. The analysis substantiates a new hypothesis postulating tumors as outgrowths of the stem cells with mesenchymal commitment lineage (mSC) which migrate from the endometrium/myometrium junctional zone of the uterus. Comparative analysis has revealed basic similarities of molecular pathogenesis of EM and UL suggesting molecular syntropy of both disorders. Peculiarities of the epigenetic landscape determining development of mSC may explain the existence of different clinical forms of EM and UL as well as their unique clinical manifestation. Some perspectives for practical and scientific application in EM and UL studies of this new hypothesis are outlined.

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Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells

Abstract: This study identified CD146 as a marker of colony-forming human endometrial stromal cells supporting the concept that human endometrium contains a population of candidate stromal stem/progenitor cells.

Cited by 182 publications

References 52 publications

Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures

Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Comparative systems genetics view of endometriosis and uterine leiomyoma: Two sides of the same coin?

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