Identification of superoxide dismutase activity in Borrelia burgdorferi

Whitehouse, Chris A.; Williams, Lisa; Austin, F E

doi:10.1128/iai.65.11.4865-4868.1997

Cited by 27 publications

(23 citation statements)

References 43 publications

(32 reference statements)

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“…60 The last misprediction concerns the Borrelia burgdorferi sod (O30563), predicted as Mn dimer and identified as Fesod on the basis of its pattern of inhibition. 61 To have an objective estimation of the predictive power of our fingerprint method, we compare it with the commonly used assignment method, where the target sequence is aligned onto sods of each type and assigned the oligomer state and metal specificity of the most similar sequence, as described in Methods Section (Table SII of Supplementary Materials). Examples of results are given in Figure 3.…”

Section: Fingerprint Prediction Methods For Sod Specificity and Oligommentioning

confidence: 99%

Mn/Fe superoxide dismutase interaction fingerprints and prediction of oligomerization and metal cofactor from sequence

2007

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Fe- and Mn-containing superoxide dismutase (sod) enzymes are closely related and similar in both amino acid sequence and structure, but differ in their mode of oligomerization and in their specificity for the Fe or Mn cofactor. The goal of the present work is to identify and analyze the sequence and structure characteristics that ensure the cofactor specificities and the oligomerization modes. For that purpose, 374 sod sequences and 17 sod crystal structures were collected and aligned. These alignments were searched for residues and inter-residue interactions that are conserved within the whole sod family, or alternatively, that are specific to a given sod subfamily sharing common characteristics. This led us to define key residues and inter-residue interaction fingerprints in each subfamily. The comparison of these fingerprints allows, on a rational basis, the design of mutants likely to modulate the activity and/or specificity of the target sod, in good agreement with the available experimental results on known mutants. The key residues and interaction fingerprints are furthermore used to predict if a novel sequence corresponds to a sod enzyme, and if so, what type of sod it is. The predictions of this fingerprint method reach much higher scores and present much more discriminative power than the commonly used method that uses pairwise sequence comparisons.

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Section: Fingerprint Prediction Methods For Sod Specificity and Oligommentioning

confidence: 99%

Mn/Fe superoxide dismutase interaction fingerprints and prediction of oligomerization and metal cofactor from sequence

2007

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“…A low ( 6 10) passage of B-31 (LP B-31) was obtained from A.G. Barbour, University of California, Irvine, CA, USA. All other borrelial strains and culture conditions have been described previously [3]. Escherichia coli QC779 lacking both genes for MnSod and FeSod (sodA and sodB) was provided by B. Bachmann, E. coli Genetic Stock Center, Yale University, New Haven, CT, USA.…”

Section: Methodsmentioning

confidence: 99%

“…Superoxide dismutase (Sod), which catalyzes the dismutation of superoxide anion (O 3 2 ) to hydrogen peroxide (H 2 O 2 ) and O 2 , is an enzymatic defense against O 2 toxicity. Bacterial Sods contain iron (FeSod or SodB), manganese (MnSod or SodA), or both copper and zinc (CuZn-Sod or SodC) [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial Sods contain iron (FeSod or SodB), manganese (MnSod or SodA), or both copper and zinc (CuZn-Sod or SodC) [1,2]. This laboratory reported that three genospecies of Lyme disease spirochetes, Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii, possess an FeSod [3]. The metal ligand was identi¢ed as Fe on the basis of the inactivation of enzymatic activity by H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi

Nichols

2000

FEMS Microbiology Letters

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A single superoxide dismutase (Sod) gene was identified in Borrelia burgdorferi strains, Borrelia afzelii Ple and Borrelia garinii Pbi. Recombinant enzymatic activity was detected only when sod expression was controlled by the lacZ promoter in the cloning vector. Northern blot analysis with sod‐ or secA‐specific probes identified a common 3.7‐kb transcript. Reverse transcriptase‐PCR analysis confirmed that secA and sod constitute a single transcriptional unit in B. burgdorferi. A transcriptional start site of this operon, containing −10 and −35 regions of a σ70‐type promoter, was mapped to 100 bp upstream of the ATG start codon of secA.

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“…Bacterial Sods contain iron (FeSod or SodB), manganese (MnSod or SodA), or both copper and zinc (CuZnSod or SodC) [1,2]. This laboratory reported that three genospecies of Lyme disease spirochetes, Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii , possess an FeSod [3]. The metal ligand was identified as Fe on the basis of the inactivation of enzymatic activity by H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of a superoxide dismutase gene ofBorrelia burgdorferi

Nichols

Whitehouse

Austin

2000

FEMS Microbiology Letters

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A single superoxide dismutase (Sod) gene was identified in Borrelia burgdorferi strains, Borrelia afzelii Ple and Borrelia garinii Pbi. Recombinant enzymatic activity was detected only when sod expression was controlled by the lacZ promoter in the cloning vector. Northern blot analysis with sod- or secA-specific probes identified a common 3.7-kb transcript. Reverse transcriptase-PCR analysis confirmed that secA and sod constitute a single transcriptional unit in B. burgdorferi. A transcriptional start site of this operon, containing -10 and -35 regions of a sigma(70)-type promoter, was mapped to 100 bp upstream of the ATG start codon of secA.

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Identification of superoxide dismutase activity in Borrelia burgdorferi

Cited by 27 publications

References 43 publications

Mn/Fe superoxide dismutase interaction fingerprints and prediction of oligomerization and metal cofactor from sequence

Mn/Fe superoxide dismutase interaction fingerprints and prediction of oligomerization and metal cofactor from sequence

Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi

Transcriptional analysis of a superoxide dismutase gene ofBorrelia burgdorferi

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