Identification of superficial zone articular chondrocyte stem/progenitor cells

Hattori, Shintaro; Oxford, Carol; Reddi, A. Hari

doi:10.1016/j.bbrc.2007.04.142

Cited by 119 publications

(113 citation statements)

References 13 publications

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“…Recently, the presence of progenitor cells in human articular cartilage has been extensively investigated [13][14][15]. Although articular cartilage develops only from embryonic mesenchyme and intervertebral discs develop from the notochord as well, these two share similarities in cellular phenotype and extracellular matrix composition [16].…”

Section: Introductionmentioning

confidence: 99%

Study to Determine the Presence of Progenitor Cells in the Degenerated Human Cartilage Endplates

Huang

Liu

et al. 2014

Global Spine Journal

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Introduction Cartilage endplate (CEP) degeneration is usually accompanied by loss of cellularity, and this loss may be a crucial key factor in initiation and development of degenerative disc disease. The study of cell types in degenerated CEP could help in understanding CEP etiopathogenesis, and may help in devising new treatments, especially if the presence of progenitor cells could be demonstrated. The aim of this study was to determine if progenitor cells existed in degenerated human CEP. Materials and methods Cells isolated from CEP were cultured in a three-dimensional agarose suspension to screen for proliferative cell clusters. Cell clusters were then expanded in vitro and the populations were analyzed for colony forming unit, immunophenotype, multilineage induction, and expression of stem cell-related genes. Results The presence of progenitor cells in degenerated human CEP is indicated by the results of CFU, immunophenotype, multilineage induction, and expression of stem cell-related genes. Conclusions We believe that this is the first study which has conclusively shown the presence of progenitor cells in degenerated CEP. The finding of this study may influence the clinical management of degenerative disc disorder.

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Section: Introductionmentioning

confidence: 99%

Study to Determine the Presence of Progenitor Cells in the Degenerated Human Cartilage Endplates

Huang

Liu

et al. 2014

Global Spine Journal

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“…8 Recent evidence also supports another major difference between the top layers of articular cartilage tissue and the lower layers: the presence of a progenitor cell population. [36][37][38][39][40] While the presence of a progenitor population within articular cartilage has now been independently reported by several groups, 36,37,39 the relative percentage of the progenitor population and the precise distribution has not been determined. Reports of the relative percentage of cells with stem cell markers range from 0.14% to 45% of the tissue's total cell population, depending on detection method and definition.…”

Section: Discussionmentioning

confidence: 99%

“…Reports of the relative percentage of cells with stem cell markers range from 0.14% to 45% of the tissue's total cell population, depending on detection method and definition. 37,39,40 At present it is unclear what the relative percentage of stem/progenitor cells is and exactly where that population is zonally distributed. However, results support that the superficial zone contains the highest relative percentage of a progenitor population compared with other zones.…”

Section: Discussionmentioning

confidence: 99%

Engineering Superficial Zone Chondrocytes from Mesenchymal Stem Cells

Coates

Fisher

2014

Tissue Engineering Part C: Methods

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Recent cartilage engineering efforts have focused on development of zonally organized tissue. However, there remains a need for protocols that differentiate progenitor populations into chondrocytes of zonal phenotype. Here, we evaluate the potential of coculture of bovine mesenchymal stem cells (MSCs) and zonal explants of bovine cartilage tissue to drive MSC differentiation to chondrocytes with the superficial zone phenotype. Two coculture systems were set up: one between alginate encapsulated MSCs and superficial zone cartilage explants, and one between MSCs and middle/deep zone cartilage explants. Chondrogenic and superficial zone markers were monitored over a 21-day differentiation period via gene and protein expression. A control conditioned media study was used to determine the impact of communication via soluble factors between cell populations during differentiation. At day 21, results show superficial zone explant coculture without transforming-growth factor b3 supplementation induces upregulation of chondrogenic gene expression markers SOX9 and type II collagen 3.4-fold and 11.4-fold, respectively, over standard chondrogenic control media. Further, coculture of MSCs and superficial zone explants can be used to upregulate mRNA expression of the superficial zone marker proteoglycan-4 in MSCs (1.75-fold over chondrogenic control at day 21), indicating the superficial zone chondrocyte phenotype. Gene expression data show middle/deep zone explant and MSC coculture did not induce the chondrogenesis observed in superficial zone explant coculture. Likewise, poor chondrogenesis was observed in all conditioned media groups. Results highlight the importance of superficial zone cartilage and cells in guiding stem cell fate and regulating differentiation of MSCs to chondrocytes of the superficial zone type.

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“…Progenitor cells within osteoarthritic cartilage and other mesenchymal osteoarthritic tissues have been described in terms of their potential for in situ regeneration [19][20][21]. It has been presumed that OA occurrence and progression could be a process of uncoordinated growth [22,23].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Phenotypic Characterisation of Stem Cells from Late Stage Osteoarthritic Mesenchymal Tissues

Lăbuşcă¹,

Zugun‐Eloae²,

Shaw³

et al. 2012

CSCR

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Abstract:Introduction: Osteoarthritis (OA) represents an increasing health issue worldwide. Regenerative medicine (RM) has raised the hope for introducing revolutionary therapies in clinical practice. Detection of autologus cell sources can improve accessibility to RM strategies.Objectives: to assess the presence and biological potential of mesehchymal stem cells in three tissues (subchondral bone, synovial layer, periarticular adipose tissue) in late stages osteoarthritic patients.Material and Methods: samples were collected from subjects undergoing total knee replacement (TKR).. MSCs were isolated and cultured in complete MEM with FGF. Cell morphology and growth potential was assessed. Flow cytometry was used for detection of several relevant cell surface markers. Quantitative and qualitative assessment of differentiation potential towards three mesenchymal lineages (osteogenesis adipogenesis chondrogenesis) was performed. Time lapse life cell imaging of nondiferentiated cells over 24 hours period was used to determine cell kinetics.Results: mesenchymal cells derived from all donors and tissue types showed morphology, growth and surface cell markers associated with stemness. All cell types underwent differentiation toward three mesenchymal lineages with significant differences between tissues of origin, not between donors. Cell kinetics, as derived from life imaging records, was variable with tissue of origin, significant higher for adipose derived MSCS. Conclusion:Human late stage OA mesenchymal tissues, contain progenitors with proliferative and differentiation potential of MSCs. These populations can be used for research and autologus regenerative therapies. Further comparative studies with age matched non OA samples has the potential of contributing to deepening knowledge about disease occurrence and progression.

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Identification of superficial zone articular chondrocyte stem/progenitor cells

Cited by 119 publications

References 13 publications

Study to Determine the Presence of Progenitor Cells in the Degenerated Human Cartilage Endplates

Study to Determine the Presence of Progenitor Cells in the Degenerated Human Cartilage Endplates

Engineering Superficial Zone Chondrocytes from Mesenchymal Stem Cells

Isolation and Phenotypic Characterisation of Stem Cells from Late Stage Osteoarthritic Mesenchymal Tissues

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