Identification of Sulfur-Containing Chlorinated Paraffin Structural Analogues in Human Serum: Origination from Biotransformation or Bioaccumulation?

Abstract

Chlorinated paraffins (CPs) are manufactured and used in high quantities and have diverse structural analogues. It is generally recognized that sulfurcontaining structural analogues of CPs are mainly derived from sulfate-conjugated phase II metabolism. In this study, we non-targeted identified three classes of sulfur-containing CP structural analogues (CPs−S) in human serum, including 44 CP sulfates (CPs− SO 4 H/CPs−SO 4 H−OH), 14 chlorinated benzene sulfates (CBs−SO 4 H), and 19 CP sulfite esters (CPs−SO 3 /CPs−S 2 O 6 ), which were generated during the production of commercial mixtures of CPs and, thus, bioaccumulated via environmental exposures. We first wrote a program to screen CPs−S, which were baseline-separated from CPs according to their polar functional groups. Then, mass spectral analyses of alkalization− acidification liquid−liquid extracts of serum samples and Orbitrap mass spectrometry analyses in the presence and absence of tetraphenylphosphonium chloride (Ph 4 PCl), respectively, were performed to determine the ionization forms ([M + Cl] − or [M − H] − ) of CPs−S. The presence of fragment ions (SO 4 H − , SO 3 − , SO 2 Cl − , and HSO 3 − ) revealed the structures of CPs−S, which were validated by their detections in commercial mixtures of CPs. The estimated total concentrations of CPs−S in the human serum samples were higher than the concentrations of medium-and long-chain CPs. The profiles of CPs−S in human serum were similar to those detected in CP commercial mixtures and rats exposed to the commercial mixtures, but CPs−S were not detected in human liver S9 fractions or rat tissues after exposure to CP standards. These results, together with the knowledge of the processes used to chemically synthesize CPs, demonstrate that CPs−S in humans originates from environmental bioaccumulation.

Enhanced Research Needed on the Structural Analogues of Chlorinated Paraffins as Emerging Contaminants of Concern

“…Semiquantitative concentrations of BCPs and BCOs in dust samples (median concentrations of 5.4 and 0.18 μg/g, respectively) are comparable to CPs, and 1–2 orders of magnitude higher than those in the atmosphere and sediments at some sites . More than 70 kinds of sulfur-containing chlorinated paraffins (S-CPs) and 399 oxygen-containing CPs (CPO x s, including 285 congeners of chlorinated fatty acid methyl esters, CFAMEs) were detected in serum samples of Chinese general population and the levels of ∑CPO x s were 202.2 ± 324.7 ng/mL with a detection frequency of 100%, which were comparable to the concentration of ∑MCCPs (142.8 ± 144.6 ng/mL). , …”

“…Structural analogues of chlorinated paraffins (CPs) are compounds that contain the chlorinated n -alkanes skeleton of CPs and other functional groups. Recently, some structural analogues of CPs at high levels were detected in the environment and general population. − The atmospheric concentrations of unsaturated short, medium, and long chain chlorinated olefins (COs) were 0.01–0.27, 0.02–0.56, and 0.01–0.55 ng/m 3 in China . Over 700 brominated-chlorinated paraffin and brominated-chlorinated olefin congeners (BCPs and BCOs) were found in different matrices in Australia.…”