Identification of Subunits for Novel Universal Vaccines against Three Predominant Serogroups and the Emerging O145 among Avian Pathogenic Escherichia coli by Pan-RV Pipeline

Abstract

With the emergence of drug resistance and the newly predominant serogroup O145, the control of Avian pathogenic Escherichia coli is facing a serious challenge; an efficient immunological method is urgently needed. Here, for the first time, we propose a high-throughput screening approach to search for universal protective antigens against the three traditional serogroups and the newly emerged O145.

Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia

“…Infectious diseases can attack poultry, one of which is ducks caused by avian pathogenic Escherichia coli (APEC) and is considered the main cause of health problems in poultry farms worldwide (Abreu et al 2023;Putri et al 2023). APEC infection causes symptoms systemic, such as airsacculitis, pericarditis, perihepatitis, septicemia, enteritis, granuloma, sinusitis, omphalitis, peritonitis, and swollen head syndrome in poultry (Azam et al 2020;Wang et al 2023). Several genes in the plasmid cause virulence factors in APEC strains, such as cvaC, tsh, sitA, iutA, ompT, etsABCD, eitABC, hlyF, and iroN.…”

Evaluation of Two Recombinant Protein-Based Vaccine Regimens against Campylobacter jejuni: Impact on Protection, Humoral Immune Responses and Gut Microbiota in Broilers

“…The pangenomic reverse vaccinology method, based on a genome-wide reverse vaccinology model using the global genetic repertoire of species, increases the possibility of identifying new vaccine candidates [57]. This strategy has already been used in chickens against other bacteria such as Gallibacterium anatis with the use of ten genomes [58] and avian pathogenic Escherichia coli with the use of 58 and 127 genomes in two studies by the same team [59,60]. Because of the elevated genetic diversity across Campylobacter serotypes [61], a genomewide reverse vaccinology model using the global genetic repertoire of thermotolerant Campylobacter species responsible for campylobacteriosis could be applied.…”