Identification of streptococci from Greek Kasseri cheese and description of Streptococcus macedonicus sp. nov.

Tsakalidou, Effie; Zoidou, Evangelia; Pot, Bruno; Wassill, Lars; Ludwig, Wolfgang; Devriese, Luc; Kalantzopoulos, George; Schleifer, K. H.; Kersters, Karel

doi:10.1099/00207713-48-2-519

Cited by 110 publications

(76 citation statements)

References 27 publications

(15 reference statements)

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“…This subspecies includes strains formerly identified as S. macedonicus Tsakalidou et al 1998or S. waius Flint et al 1999. The strains are positive for b-galactosidase (b-GAR test), negative for b-glucosidase and they do not hydrolyse aesculin.…”

Section: Discussionmentioning

confidence: 99%

Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov.

Schlegel

Grimont

Ageron

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

341

278

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Section: Discussionmentioning

confidence: 99%

Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov.

Schlegel

Grimont

Ageron

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

341

278

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“…The latter show a similar or a slightly atypical carbohydrate fermentation pattern, compared with those of S. thermophilus, but are a different genotype. The taxonomic position of these anomalous strains has been clarified starting from the description of the species Streptococcus macedonicus and Streptococcus waius (Flint et al, 1999;Tsakalidou et al, 1998). These species are phylogenetically located in the Streptococcus bovis rRNA group while S. thermophilus is closely related to Streptococcus salivarius.…”

Section: Introductionmentioning

confidence: 99%

16S–23S rRNA intergenic spacer region sequence variation in Streptococcus thermophilus and related dairy streptococci and development of a multiplex ITS-SSCP analysis for their identification

et al. 2003

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The 16S-23S rRNA internal transcribed spacer (ITS) region of several Streptococcus thermophilus strains and some related dairy streptococci, S. macedonicus, S. salivarius and S. bovis, was analysed by sequence analysis. All the Streptococcus species were easily discriminated on the basis of sequence variations principally located upstream and downstream of the region encompassing the double-stranded processing sites and the tRNA Ala gene. Comparison between tRNA Ala gene sequences highlighted a high level of sequence conservation among the Streptococcus species investigated despite their belonging to separated phylogenetic clusters, i.e. the S. salivarius and S. bovis rRNA groups. A low but significant degree of variability was detected among the S. thermophilus strains, allowing the identification of four different ITS sequences. Similarity analysis of the ITS sequences showed that the Streptococcus species were clustered in two main branches, one containing S. macedonicus and S. bovis strains, and one containing S. thermophilus and S. salivarius strains. With the aim of developing a rapid tool for the identification of the dairy streptococci species a multiplex ITS-SSCP analysis of two discrete regions within the ITS locus was carried out.

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“…The inherent problem of this approach is the large number of species relative to the limited number of biochemical traits that can be analyzed, the variability of several traits within species (33,35,36,44), the poor reproducibility of some tests (12,17,26,36,44), and the lack of sufficient phenotypic data on more recently described species in the underlying databases. The last problem applies to the species S. cristatus (23), S. peroris, S. infantis (31), S. australis (55), S. sinensis (57), Streptococcus macedonicus (51), Streptococcus infantarius, Streptococcus lutetiensis, Streptococcus gallolyticus (42), and S. pseudopneumoniae (1).…”

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confidence: 99%

Use of Phylogenetic and Phenotypic Analyses To Identify Nonhemolytic Streptococci Isolated from Bacteremic Patients

2005

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The aim of this study was to evaluate molecular and phenotypic methods for the identification of nonhemolytic streptococci. A collection of 148 strains consisting of 115 clinical isolates from cases of infective endocarditis, septicemia, and meningitis and 33 reference strains, including type strains of all relevant Streptococcus species, were examined. Identification was performed by phylogenetic analysis of nucleotide sequences of four housekeeping genes, ddl, gdh, rpoB, and sodA; by PCR analysis of the glucosyltransferase (gtf) gene; and by conventional phenotypic characterization and identification using two commercial kits, Rapid ID 32 STREP and STREPTOGRAM and the associated databases. A phylogenetic tree based on concatenated sequences of the four housekeeping genes allowed unequivocal differentiation of recognized species and was used as the reference. Analysis of single gene sequences revealed deviation clustering in eight strains (5.4%) due to homologous recombination with other species. This was particularly evident in S. sanguinis and in members of the anginosus group of streptococci. The rate of correct identification of the strains by both commercial identification kits was below 50% but varied significantly between species. The most significant problems were observed with S. mitis and S. oralis and 11 Streptococcus species described since 1991. Our data indicate that identification based on multilocus sequence analysis is optimal. As a more practical alternative we recommend identification based on sodA sequences with reference to a comprehensive set of sequences that is available for downloading from our server. An analysis of the species distribution of 107 nonhemolytic streptococci from bacteremic patients showed a predominance of S. oralis and S. anginosus with various underlying infections.The genus Streptococcus currently consists of more than 50 species, most of which belong to one of six phylogenetic clusters that are revealed by comparative analysis of 16S rRNA gene sequences. In addition to the pyogenic group, which includes the traditional pathogenic species (i.e., hemolytic streptococci), these clusters are the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group (30,34). Many of the species of these five clusters are major constituents of the commensal microbiota of the human oral cavity and upper respiratory tract and are occasionally implicated in various pathologies. The anginosus group, formerly called "Streptococcus milleri" in some parts of the world (16), includes three recognized species (Streptococcus anginosus, Streptococcus intermedius, and Streptococcus constellatus) that are primarily associated with suppurative infections of tissues of the mouth and various body sites, including the meninges (9,37,44,54,56). The mitis group currently includes

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Identification of streptococci from Greek Kasseri cheese and description of Streptococcus macedonicus sp. nov.

Cited by 110 publications

References 27 publications

Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov.

Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov.

16S–23S rRNA intergenic spacer region sequence variation in Streptococcus thermophilus and related dairy streptococci and development of a multiplex ITS-SSCP analysis for their identification

Use of Phylogenetic and Phenotypic Analyses To Identify Nonhemolytic Streptococci Isolated from Bacteremic Patients

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