Identification of Strains of Campylobacter jejuni and Campylobacter coli by PCR and Correlation with Phenotypic Characteristics

Occhialini, Alessandra; Stonnet, V.; Hua, Jian; Camou, Christine; Guesdon, Jean‐Luc; Mégraud, Françis

doi:10.1007/978-1-4757-9558-5_41

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“…The primers used, THERM1 and THERM 4 (Table 6), are based on the most variable part of the 23S rRNA gene, and amplify a 491-bp sequence from all 4 thermophilic species. Occhialini et al (1996) compared the efficiency of identification of C. jejuni and C. coli with two sets of PCR primers (VS15/VS16 for C. jejuni and CSF/ CSR for C. coli), each specific for each species (Table 6) to that of the Api Campy (Biomérieux) test kit. Good orrelation was obtained.…”

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confidence: 99%

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Levin

2007

Food Biotechnology

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Campylobacter jejuni is considered to be the leading cause of enteric illness in the United States and other industrialized nations, causing mild to severe symptoms including serious infections of the extremities and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry, where the intestine is colonized resulting in healthy animals as carriers. Wildlife have long been considered an infectious reservoir for campylobacters because of their close association with and contamination of surface waters. This review deals with the areas of: phenotypic characteristics of C. jejuni and related human pathogenic species of Campylobacter, their ecological distribution, virulence factors, isolation of C. jejuni from foods, serotyping of Campylobacter isolates, bacteriophage typing, molecular methods of detecting and typing campylobacters, the viable but nonculturable state of campylobacters, the coccoid form of C. jejuni and immunomagnetic capture of C. jejuni.

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Section: Downloaded By [Purdue University] At 14:28 13 April 2015mentioning

confidence: 99%

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Levin

2007

Food Biotechnology

View full text Add to dashboard Cite

show abstract

Identification of Strains of Campylobacter jejuni and Campylobacter coli by PCR and Correlation with Phenotypic Characteristics

Cited by 1 publication

References 3 publications

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

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