Identification of Stem Cells in the Epithelium of the Stomach Corpus and Antrum of Mice

Matsuo, Junichi; Kimura, Shigeo; Yamamura, Akihiro; Koh, Cai Ping; Hossain, Zakir; Heng, Dede Liana; Kohu, Kazuyoshi; Voon, Dominic Chih-Cheng; Hayashi, Hiroshi; Unno, Michiaki; So, Jimmy Bok Yan; Zhu, Feng; Srivastava, Supriya; Teh, Ming; Yeoh, Khay Guan; Osato, Motomi; Ito, Yoshiaki

doi:10.1053/j.gastro.2016.09.018

Cited by 126 publications

(144 citation statements)

References 54 publications

(59 reference statements)

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“…Stem cells in the corpus express Sox2, Troy, Mist1, and eR1, whereas stem cells in the pyloric antrum express Sox2, Lgr5, CCK2R, eR1, Axin2, and Mist1 (18-25). Mouse model studies have shown that oncogenic events in gastric stem cells can frequently lead to carcinogenesis, although mutations targeted to chief cells also can cause these cells to dedifferentiate and acquire the potential to grow and regenerate gastric glands, sometimes forming metaplasia (17,21,22,(26)(27)(28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

YAP/TAZ Initiates Gastric Tumorigenesis via Upregulation of MYC

et al. 2018

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Section: Introductionmentioning

confidence: 99%

YAP/TAZ Initiates Gastric Tumorigenesis via Upregulation of MYC

et al. 2018

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“…Lgr5-expressing cells represent long-lived progenitor cells in the gastric antrum, but they do not contribute to corpus gland cell lineage differentiation 15. Most recently, Matsuo et al 16 have used an enhancer region of the Runx1 promoter (eR1) to mark endogenous stem cells in both the corpus and antrum of the stomach.…”

Section: Introductionmentioning

confidence: 99%

Lrig1+ gastric isthmal progenitor cells restore normal gastric lineage cells during damage recovery in adult mouse stomach

Choi

Lantz

Vlacich

et al. 2017

Gut

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Objective Lrig1 is a marker of proliferative and quiescent stem cells in the skin and intestine. We examined whether Lrig1-expressing cells are long-lived gastric progenitors in gastric glands in the mouse stomach. We also investigated how the Lrig1-expressing progenitor cells contribute to the regeneration of normal gastric mucosa by lineage commitment to parietal cells after acute gastric injury in mice. Design We performed lineage labelling using Lrig1-CreERT2/+;R26R-YFP/+ (Lrig1/YFP) or R26R-LacZ/+ (Lrig1/LacZ) mice to examine whether the Lrig1-YFP-marked cells are gastric progenitor cells. We studied whether Lrig1-YFP-marked cells give rise to normal gastric lineage cells in damaged mucosa using Lrig1/ YFP mice after treatment with DMP-777 to induce acute injury. We also studied Lrig1-CreERT2/CreERT2 (Lrig1 knockout) mice to examine whether the Lrig1 protein is required for regeneration of gastric corpus mucosa after acute injury. Results Lrig1-YFP-marked cells give rise to gastric lineage epithelial cells both in the gastric corpus and antrum, in contrast to published results that Lgr5 only marks progenitor cells within the gastric antrum. Lrig1-YFP-marked cells contribute to replacement of damaged gastric oxyntic glands during the recovery phase after acute oxyntic atrophy in the gastric corpus. Lrig1 null mice recovered normally from acute gastric mucosal injury indicating that Lrig1 protein is not required for lineage differentiation. Lrig1+ isthmal progenitor cells did not contribute to transdifferentiating chief cell lineages after acute oxyntic atrophy. Conclusions Lrig1 marks gastric corpus epithelial progenitor cells capable of repopulating the damaged oxyntic mucosa by differentiating into normal gastric lineage cells in mouse stomach.

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“…Here, Leushacke et al observed that a subset of Lgr5+ chief cells located at the gland base reconstituted whole glands in response to damage with high doses of tamoxifen In the isthmus, Troy and Mist1 might be expressed by the same cells and both populations are known to act as a cell of origin for gastric tumourigenesis 6 . Although Runx1 expression was not analysed by Leushacke et al, a small population of Runx1+ cells with stem cell properties was previously found in the base of the corpus, suggesting Lgr5 and Runx1 could label a common reserve stem cell in this location 7 . Runx1 and Mist1 are both expressed in the isthmus, however, Runx1 cells are highly proliferative, whereas Mist1 cells are quiescent, and for this reason it is likely that these two genes label distinct populations of stem cells in the isthmus.…”

Section: Mist1+mentioning

confidence: 92%

“…, Mist1 6 and Runx1 7 all mark cells that can trace full corpus glands originating from the isthmus [5][6][7] . However, they have all been reported to be expressed in the base of the corpus also, with basally located Troy+ cells giving rise to fully labelled glands on rare occasions 6 , and also acting as a reserve stem cell after injury 8 .…”

mentioning

confidence: 99%