Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labeling

Wei, Xia-fei; Fan, Shu-ying; Wang, Yuwei; Li, Shan; Long, Shao-yuan; Gan, Chun-Yang; JieLi,; Sun, Yuxue; Guo, Lin; Wang, Pei-yun; Yang, Xue; Wang, Jinlan; Cui, Jing; Zhang, Wenlu; Huang, Aiping; Hu, Jieli

doi:10.1016/j.isci.2022.104416

Cited by 7 publications

(8 citation statements)

References 94 publications

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“…Biotinylated proteins were enriched from the cell lysates using streptavidin magnetic beads, and then LC‐MS analysis was performed to identify proteins interacting with TIM22 and TIM29. The concentration and time of biotin and rapamycin treatment have been optimized in a previous study 22 …”

Section: Resultsmentioning

confidence: 99%

“…The concentration and time of biotin and rapamycin treatment have been optimized in a previous study. 22 As shown in Figure 4B, a total of 68 proteins were identified in rapamycin-treated FRB-TIM22 samples, of which 42 do not overlap with the control group. A total of 74 proteins were identified in the FRB-TIM29 sample, of which 48 do not overlap with the control group.…”

Section: Identification Of Host Factors Interacting With Tim22/tim29mentioning

confidence: 91%

“…The original HBV1.1 ploidy gene sequence was replaced and constructed by molecular cloning. Plasmids pFKBP12‐TurboID were constructed by us previously 21,22 …”

Section: Methodsmentioning

confidence: 99%

“…Plasmids pFKBP12-TurboID were constructed by us previously. 21,22 2.2 | Cell culture and transfection Huh7, HepG2, HepG2-NTCP, HepG2.2.15, and HepAD38 cells were grown in DMEM supplemented with 10% fetal bovine serum, 100 U/ mL penicillin, and 100 mg/mL streptomycin at 37°C, 5% CO 2 .…”

Section: Plasmid Constructionmentioning

confidence: 99%

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TIM22 and TIM29 inhibit HBV replication by up‐regulating SRSF1 expression

Guo,

Liu,

Long

et al. 2024

Journal of Medical Virology

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Hepatitis B virus (HBV) infection is a serious global health problem. After the viruses infect the human body, the host can respond to the virus infection by coordinating various cellular responses, in which mitochondria play an important role. Evidence has shown that mitochondrial proteins are involved in host antiviral responses. In this study, we found that the overexpression of TIM22 and TIM29, the members of the inner membrane translocase TIM22 complex, significantly reduced the level of intracellular HBV DNA and RNA and secreted HBV surface antigens and E antigen. The effects of TIM22 and TIM29 on HBV replication and transcription is attributed to the reduction of core promoter activity mediated by the increased expression of SRSF1 which acts as a suppressor of HBV replication. This study provides new evidence for the critical role of mitochondria in the resistance of HBV infection and new targets for the development of treatment against HBV infection.

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Host Factors Interacting With Tim22/tim29mentioning

confidence: 91%

“…The original HBV1.1 ploidy gene sequence was replaced and constructed by molecular cloning. Plasmids pFKBP12‐TurboID were constructed by us previously 21,22 …”

Section: Methodsmentioning

confidence: 99%

Section: Plasmid Constructionmentioning

confidence: 99%

See 2 more Smart Citations

TIM22 and TIM29 inhibit HBV replication by up‐regulating SRSF1 expression

Guo,

Liu,

Long

et al. 2024

Journal of Medical Virology

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“…Compared with conventional Y2H and Co-IP methods, TurboID-based PL allows for rapid, nontoxic PL of target proteins in living cells at room temperature (RT) and shows a distinct advantage in identifying transient, weak, or hydrophobic PPIs ( Branon et al 2018 ; Mair et al 2019 ; Zhang et al 2019 ; Arora et al 2020 ; Yang et al 2021 ; Xu et al 2023 ). Although TurboID-based PL has been used to dissect the VRCs of several animal viruses ( Kumar et al 2021 ; Fang et al 2022a , b ; Liu et al 2022 ; Wei et al 2022 ; Zhang et al 2022 ), few studies have employed this technique to analyze VRCs in planta .…”

Section: Introductionmentioning

confidence: 99%

RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants

et al. 2023

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Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. TurboID-based proximity labeling (PL) has emerged as a robust tool for probing molecular interactions in planta. However, few studies have employed the TurboID-based PL technique for investigating plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum (ER)-replicating virus, as a model, and systematically investigated the composition of BBSV VRCs in Nicotiana benthamiana by fusing the TurboID enzyme to viral replication protein p23. Among the 185 identified p23-proximal proteins, the reticulon family of proteins showed high reproducibility in the mass spectrometry datasets. We focused on RETICULON-LIKE PROTEIN B2 (RTNLB2) and demonstrated its pro-viral functions in BBSV replication. We showed that RTNLB2 binds to p23, induces ER membrane curvature, and constricts ER tubules to facilitate the assembly of BBSV VRCs. Our comprehensive proximal interactome analysis of BBSV VRCs provides a resource for understanding plant viral replication and offers additional insights into the formation of membrane scaffolds for viral RNA synthesis.

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A TurboID-based proximity labelling approach for identifying the DNA-binding proteins

Wei

2023

STAR Protocols

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Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labeling

Cited by 7 publications

References 94 publications

TIM22 and TIM29 inhibit HBV replication by up‐regulating SRSF1 expression

TIM22 and TIM29 inhibit HBV replication by up‐regulating SRSF1 expression

RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants

A TurboID-based proximity labelling approach for identifying the DNA-binding proteins

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