Identification of stathmin 1 during peri-implantation period in mouse endometrium by a proteomics-based analysis

Gou, Jinhai; Jia, Jia; Zhao, Xia; Yi, Tao; Li, Zhengyu

doi:10.1016/j.bbrc.2015.02.171

Cited by 9 publications

(13 citation statements)

References 13 publications

(15 reference statements)

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“…Mature mice (C57BL6/J mice, 6-8 weeks of age and 18-20 g each) were housed in a controlled environment with a 12 h light/12 h dark cycle and free access to food and water. Models of pregnancy, pseudo-pregnancy, delayed implantation, artificial decidualization and ovariectomy followed by hormone replacement, were established as described in our recent report (Gou et al, 2015). Pregnant mice were obtained by mating 7-9 week old females with mature males at 20:00 h, and the next morning, when vaginal plug was observed, was designated as day 1 of pregnancy.…”

Section: Establishment Of Animal Modelsmentioning

confidence: 99%

“…Tissue samples were ground into powder in liquid nitrogen and lysed in RIPA lysis buffer, and the experiment was performed as described in our recent report (Gou et al, 2015). The specific antibodies used were goat anti-mouse apoA1 (1:2000, Abcam, Cambridge, UK), rabbit anti-mouse hnRNP-E1 (1:1000, Abcam), and rabbit anti-mouse eNOS (1:1000, Cell Signaling Technologies, Beverly, MA).…”

Section: Western Blotmentioning

confidence: 99%

“…We recently presented the protein profiles of murine endometrium during implantation, and observed the upregulation of apoA1 and hnRNP-E1 (Gou et al, 2015). Consequently, based on these findings, this study further examined the expression profiles of apoA1 and hnRNP-E1 in a series of established models and the knockdown effect on embryo implantation and lipid peroxidation, aiming to validate the hypothesis that apoA1 and hnRNP-E1 might be involved in the process of lipid peroxidation, and serve as potential regulators of embryo implantation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation

Jia

Gou

Zhao

et al. 2016

Reproductive BioMedicine Online

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It is known that apolipoprotein A1 (apoA1) is a stimulator of endothelial nitric oxide synthase (eNOS), and that heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1)-containing RNP complexes is a key protector of basal stabilization of eNOS mRNA. Recently, we found that apoA1 and hnRNP-E1 were up-regulated during peri-implantation period, and the purpose of this study was to explore the roles of apoA1 and hnRNP-E1 during this period in the mouse. It was found that the up-regulation of apoA1 and hnRNP-E1 were dependent on the presence and status of blastocysts, on endometrial decidualization and on the progesterone and 17β-oestradiol status. Knockdown of apoA1 or hnRNP-E1 both resulted in reduced numbers of embryo implantations and neonates (P < 0.01), and lipid peroxidation was found to be involved. On pregnancy day 5 eNOS expression and superoxidase dismutase (SOD) quantity were increased, and malondialdehyde (MDA) quantity was decreased at implantation sites. The knockdown of either apoA1 or hnRNP-E1 led to down-regulation of eNOS (P < 0.01) and to an increase in the quantity of MDA (P < 0.05), and a decrease in the amount of SOD (P < 0.01). These findings suggest that apoA1 and hnRNP-E1 may play roles in embryo implantation by inhibiting lipid peroxidation.

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Section: Establishment Of Animal Modelsmentioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation

Jia

Gou

Zhao

et al. 2016

Reproductive BioMedicine Online

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“…A downregulation of the expression of this HDL has been associated with an increased risk of pre-eclampsia in humans [23], and its dysfunction enhances embryo toxicity by increasing cell susceptibility to protein degradation and structural modifications [24]. Physiologically, this gene was found upregulated in the endometrium of rodents during the implantation period [25], and it seems to promote several mechanisms to achieve a successful implantation process [26]. Moreover, an overexpression of the protein ApoA-I has been found in human early embryos and cultured blastocysts deemed of superior quality [27], which reinforces the idea that this protein is closely related with embryonic quality.…”

Section: Discussionmentioning

confidence: 99%

Seminal Plasma Induces Overexpression of Genes Associated with Embryo Development and Implantation in Day-6 Porcine Blastocysts

Martínez

Cambra

Gil

et al. 2020

IJMS

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The infusion of boar seminal plasma (SP) before artificial insemination (AI) positively alters the expression of endometrial genes and pathways involved in embryo development. This study aimed to determine which transcriptome changes occur in preimplantation embryos in response to SP infusions during estrus. Postweaning estrus sows received 40-mL intrauterine infusions of either SP (N = 6) or BTS extender (control group; N = 6) 30 min before each of two post-cervical AIs. On Day 6, embryos were surgically collected and analyzed for differential gene expression. Microarray analysis of embryos revealed 210 annotated genes, differentially expressed (p-value < 0.05 and fold change </> 2) in SP-blastocysts, compared to controls. Most of these genes were associated with biological, cellular, metabolic and developmental processes. The pathways enriched among the upregulated genes related to signal transduction, cellular processes and the endocrine system. Among altered genes involved in these pathways, the SP-group showed a conspicuous overexpression of ApoA-I, CDK1, MAPK1, SMAD2, PRKAA1 and RICTOR, with reported key roles in embryo development, implantation, or progression of pregnancy. In conclusion, the results demonstrate that SP infusions prior to AI upregulates the expression of embryo development related genes in Day 6 pig embryos.

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“…Other interesting genes were ApoA1, PRKAA1 and RICTOR. The ApoA1 gene encodes apolipoprotein A-I, a principal constituent of high-density lipoprotein, which has been shown to be upregulated in the murine endometrium during implantation (Gou et al, 2015). Moreover, it has been suggested that ApoA1 plays important roles in embryo implantation by inhibiting lipid peroxidation (Jia et al, 2016).…”

Section: Seminal Plasma and Preimplantation Embryosmentioning

confidence: 99%

Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine

et al. 2020

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Seminal plasma (SP) supports not only sperm function but also the ability of spermatozoa to withstand biotechnological procedures as artificial insemination, freezing or sex sorting. Moreover, evidence has been provided that SP contains identifiable molecules which can act as fertility biomarkers, and even improve the output of assisted reproductive technologies by acting as modulators of endometrial and embryonic changes of gene expression, thus affecting embryo development and fertility beyond the sperm horizon. In this overview, we discuss current knowledge of the composition of SP, mainly proteins and cytokines, and their influence on semen basic procedures, such as liquid storage or cryopreservation. The role of SP as modulator of endometrial and embryonic molecular changes that lead to successful pregnancy will also be discussed.

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Identification of stathmin 1 during peri-implantation period in mouse endometrium by a proteomics-based analysis

Cited by 9 publications

References 13 publications

Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation

Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation

Seminal Plasma Induces Overexpression of Genes Associated with Embryo Development and Implantation in Day-6 Porcine Blastocysts

Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine

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