Identification of stable sources of resistance to mungbean yellow mosaic virus (MYMV) disease in mungbean [Vigna radiata (L.) Wilczek]

Abstract

Yellow mosaic disease (YMD) caused by mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in mungbean. Development and introduction of resistant cultivars are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance is a pre-requisite. A set of 14 mungbean genotypes including a susceptible check were evaluated for responses to YMD under natural infection across three seasons and under challenged inoculation in glasshouse for one season. None of the genotypes were immune to YMD and produced different degrees of response to MYMV in terms of yellow mosaic symptoms (YMS). Based on the delayed appearance of initial YMS, and lower estimates of per cent disease index and area under disease progressive curve (AUDPC) in response to natural infection and challenged inoculation, five genotypes namely AVMU 1698, AVMU 1699, AVMU 16100, AVMU 16101 and KPS 2 were identified as resistant to YMD. Failure of detection of MYMV through polymerase chain reaction (PCR) using MYMV coat protein gene-specific primer and successful detection of the same through rolling circle amplification-PCR suggested latent infection of MYMV in resistant genotypes. The resistance response of the five genotypes could be attributed to enhanced activities of enzymes such as peroxidase, polyphenol oxidase and phenylalanine ammonia lyase and increased concentration of total phenols. These results are discussed in relation to strategies to breed mungbean for resistance to YMD.

Screening of Urdbean Germplasm for High Seed Yield Coupled with Resistance to Mungbean yellow mosaic virus (MYMV)

“…This study indicates the potentiality of these genotypes as resistance donors. Similarly, several mungbean genotypes were screened previously [12,13] and only two Meha and ML-1477 were found resistant in the Jharkhand region.…”

Inheritance of mungbean yellow mosaic virus (MYMV) disease resistance in mungbean under natural infection conditions

“…The basic material consisted of four MYMV-resistant cultivated genotypes namely AVMU 1698, AVMU 1699, AVMU 16100 and AVMU 16101, and MYMV-susceptible genotype, ‘Harsha’ (Nagaraj et al ., 2019). Using the four MYMV-resistant genotypes as male parents and Harsha as the female parent, four crosses namely (1) Harsha × AVMU 1698, (2) Harsha × AVMU 1699, (3) Harsha × AVMU 16100 and (4) Harsha × AVMU 16101 were synthesized during the 2018 rainy season at the experimental plots of the Department of Genetics and Plant breeding (GPB), University of Agricultural Sciences, (UAS), Bengaluru, India.…”

“…Mungbean [ Vigna radiata (L.) Wilczek] is one of the important grain legume crops extensively grown in Southeast Asia. However, its production is severely constrained by mungbean yellow mosaic virus (MYMV) disease (Kang et al ., 2005; Nagaraj et al ., 2019) which is transmitted by whitefly, Bemisia tabaci Genn. Hemiptera: Aleyrodidae (Butler, 1977).…”

Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration

“…The evaluation identified ‘ML 1628’ as a source of high resistance against MYMV ( Nair et al., 2017 ). Basavaraj et al. (2019) screened 14 mungbean genotypes over three seasons for MYMV resistance, reporting five genotypes (AVMU 1698, AVMU 1699, AVMU 16100, AVMU 16101, and KPS) with resistance.…”

“…The evaluation identified 'ML 1628' as a source of high resistance against MYMV (Nair et al, 2017). Basavaraj et al (2019) screened 14 mungbean genotypes over three seasons for MYMV resistance, reporting five genotypes (AVMU 1698, AVMU 1699, AVMU 16100, AVMU 16101, and KPS) with resistance. A rigorous field screening of a diverse set of 344 urdbean genotypes was tested for YMV resistance under field conditions for two years (Bag et al, 2014).…”