Identification of SSR &amp; ISSR Markers Associated to Off-Type Somaclonal Variants in Micro-Propagated Banana

Tarafdar, Shubhankar; Gupta, Kashmira; Banerjee, Shubha; Shailja, D.

doi:10.9790/264x-03021822

Cited by 2 publications

References 6 publications

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Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

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The CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. The ITC also serves as a vital safety backup and transit centre for national banana genebanks and ensures that germplasm is clean of pests and diseases and freely available under the International Treaty on Plant Genetic Resources for Food and Agriculture. In more than 35 years of activity, the ITC has distributed over 18,000 banana accession samples to researchers and farmers in 113 countries. Ex situ conservation of vegetatively-propagated crops such as banana poses very particular challenges. Maintaining the ITC genebank is labor intense and costly. Efficiencies are sought through research and development of techniques on detecting viruses, the genetic integrity of accessions, and on innovative means of safeguarding banana diversity, such as conserving populations of wild species by seed banking. Although the conservation of global banana diversity is the main objective of the ITC, significant value comes from its holistic approach to better understand and promote its germplasm through numerous research activities and resources. Techniques for morphological and molecular characterization serve to identify and describe the collection, while also determining what gaps should be filled by collecting missions with national partners. The evaluation of desirable agronomic traits inherent in Musa spp. are investigated by a high-throughput phenotyping platform, which helps breeding programs to select cultivars resistant or tolerant to biotic and abiotic stresses. Genomic and bioinformatic studies of several banana wild relatives greatly enhance our understanding of Musa genetic diversity, links to important phenotypic traits and bring new methods for management of the collection. Collectively, these research activities produce enormous amounts of data that require curation and dissemination to the public. The two information systems at the ITC, Musa Genebank Management System and the Musa Germplasm Information System, serve to manage the genebank activities and to make public germplasm-related data for over 30 banana collections worldwide, respectively. By implementing the 10-year workplan set out in the Global Strategy for the Conservation and Use of Musa Genetic Resources, the network MusaNet supports Musa researchers and stakeholders, including the ITC, and most importantly, links to the world’s banana-producing countries via three regional banana networks.

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Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

View full text Add to dashboard Cite

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Molecular Markers Used in the Analysis of Somaclonal Variation During Plant Tissue Culture

Núñez-Pastrana,

Cruz-Cruz,

Rodríguez-Deméneghi

et al. 2023

Genome Size and Genetic Homogeneity of Regenerated Plants: Methods and Applications

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The study of somaclonal variation is a growing research area that has allowed to identify several biological processes involved in genetic instability during plant tissue culture. These changes may be undesirable during the micropropagation of elite plants or desirable during plant breeding programs. There are different molecular techniques that allow to analyze this somaclonal variation. Due to the progress that has been made in the manipulation and analysis of DNA, the number of molecular markers has increased to achieve this objective. These methods have been increasing in number, while some of them have been widely used since their development [simple sequence repeat (SSR), inter simple sequence repeats (ISSRs), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD)], others, such as retrotransposon amplification, SSR-markers derived from expressed sequence tags, targeted region amplification polymorphism (TRAP), transcribed sequences (RNAseq). Whole genome sequencing is increasing their use and they complement each other by providing more information, allowing to link genetic markers with specific phenotypes in somaclonal variants. The aim of this chapter is to highlight the methodology of the most commonly used molecular markers to assess somaclonal variation during plant tissue culture.

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Identification of SSR & ISSR Markers Associated to Off-Type Somaclonal Variants in Micro-Propagated Banana

Cited by 2 publications

References 6 publications

Safeguarding and using global banana diversity: a holistic approach

Safeguarding and using global banana diversity: a holistic approach

Molecular Markers Used in the Analysis of Somaclonal Variation During Plant Tissue Culture

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