Identification of spring viraemia in carp (Cyprinus carpio L) in Great Britain

Bucke, D.; Finlay, John

doi:10.1136/vr.104.4.69

Cited by 14 publications

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“…SVC is regarded as exotic to Great Britain, although the virus was isolated there in 1977 (Bucke & Finlay 1979), and the disease has occurred since in isolated O Inter-Research 1994…”

Section: Introductionmentioning

confidence: 99%

“…SVC is regarded as exotic to Great Britain, although the virus was isolated there in 1977 (Bucke & Finlay 1979), and the disease has occurred since in isolated holding facilities, usually being associated with imported fish. In each case the holding facilities were successfully disinfected with no further recurrence of infection.…”

Section: Introductionmentioning

confidence: 99%

“…However, it has been demonstrated that fish populations previously exposed to a virus could be identified by a n antibody detection method when they were negative by virus isolation (Hattenberger-Baudouy et al 1989b). As blood samples can b e taken without killing fish, the use of antibody detection methods avoids the unnecessary sacrificing of older, more valuable fish for sampling purposes.SVC is regarded as exotic to Great Britain, although the virus was isolated there in 1977 (Bucke & Finlay 1979), and the disease has occurred since in isolated O Inter-Research 1994 …”

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confidence: 99%

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Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay

Dixon¹,

Hattenberger-Baudouy²,

Way³

1994

Dis. Aquat. Org.

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A compehtive lmmunoassay for the detection of spnng viraema of carp vlrus (SVCV) antibodies in fish was developed in order to screen fish stocks for previous exposure to the virus SVCV has been isolated from different fish species and the use of a competitive immunoassay overcomes the requirement of standard immunoassays for antisera prepared against the immunoglobulin of each species under test instead, the test fish serum competes with an antiserum against SVCV for binding sites on the virus The competitive assay was developed and tested using sera from common carp containing antibodies that had been induced either experimentally, or as a result of field exposure to the virus When immunoassay results were compared with results of virus neutral~sation tests on the sera from 2 groups of experimentally challenged fish there was no correlation between the 2 techniques Slmilar results were obtained with 4 groups of field-collected sera One g] oup had 34 6 % neutralisation-positive sera, but 88 5 % immunoassay-positive sera The 3 remaining groups were negative by virus neutralisation, but each group had a small number (11 4 8 3 and 11 5 % respectively) of irnrnunoassay-pos~tive sera The competitive immunoassay thus appears to be more sensitive than the neutralisation test The assay has potent~al for use in large-scale screening of fish populations but further validation on a wider range of sera is needed KEY WORDS. Spring viraemia of carp virus . Antibody detection . Competitive immunoassay INTRODUCTIONIn recent years there has been a growing interest in the health screening of fish by means of detecting specific antibodies against fish pathogens (Dixon 1989, Groot & Dixon 1989, Hattenberger-Baudouy et al. 198913, J~rrgensen et al. 1991. This is partly because of national and international legislation or policy on fish health monitoring. In addition, the wider use of immunoassay techniques employing 96-well microplates makes large-scale screening for antibodies feasible and, particularly in the case of viruses, more economical than standard pathogenisolation methods. With certain fish virus diseases, notably those caused by rhabdoviruses, it is not possible to isolate virus from carrier fish at all times of the year. For instance, infectious haematopoietic necrosis virus (IHNV) can usually only be isolated from clinically diseased fry or from adults at spawning time (Mulcahy et al. 1984); viral haemorrhagic septicaemia virus (VHSV) can only be isolated from infected fish for a short period after the onset of clinical symptoms at high water temperatures (e.g. 20°C), whereas at low water temperatures (e.g. 5"C), the virus can be isolated for a much greater period ( J~r g e n s e n 1992); and spring viraemia of carp virus (SVCV) can usually only b e detected during outbreaks of clinical disease (Dresenkamp 1986) which predominantly occur at water temperatures between 11 and 17°C (Fijan 1988). However, it has been demonstrated that fish populations previously exposed to a virus could be identified by a n antib...

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“…SVC is regarded as exotic to Great Britain, although the virus was isolated there in 1977 (Bucke & Finlay 1979), and the disease has occurred since in isolated O Inter-Research 1994…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay

Dixon¹,

Hattenberger-Baudouy²,

Way³

1994

Dis. Aquat. Org.

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“…The chronic or ulcerative form was later named "carp erythrodermatitis" and was shown to be caused by a non-motile bacterium of the genus Aeromonas (Bootsma et al, 1977). The initial isolation of Rhabdovirus carpio was followed by confirming isolations in Czechoslovakia (Macura et al, 1973;Tesarcik, 1977) in Hungary (BCktsi andSzabo, 1977, 1979) and in Great Britain (Bucke and Finlay, 1979). During the 198Os, the protozoan aetiology of swimbladder inflammation (SBI) was proved (Csaba et al, 1984;Korting, 1982;Kovacs-Gayer, 1983) and the possible role of SVCV in SBI was no longer considered.…”

Section: Spring Viraemia Of Carpmentioning

confidence: 99%

Recent achievements in studies on diseases of common carp (Cyprinus carpio L.)

Jeney

1995

Aquaculture

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Parasitic, fungal, bacterial and viral diseases of common carp (Cyprinus carpio L.) are reviewed. Besides a genera1 overview of parasites of carp, swimbladder inflammation, caused by Sphaerospora spp., is discussed in detail. Saprolegnia spp. is the most important fungal pathogen. Aeromonas hydrophila and the atypical Aeromonas salmonicida, as well as Flexibactercolumnaris, are described as the major bacterial pathogens of carp. Spring viraemia of carp caused by Rhabdouirus carpio is presented as the main viral infection of common carp. Details on methods of treatment and prevention are presented together with a description of the given diseases. The role of environmental stress, including "normal" culture practice and pollution, in the outbreak of diseases of common carp is discussed. Prospective methods to minimize the risk of diseases as well as their limitations are presented. * Corresponding author. 0044-8486/95/$09.50 0 1995 Elsevier Science B.V. All rights reserved SSD,OO44-8486(94)00283-5

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“…SVCV was first isolated in the early 1970s in Yugoslavia and has until now been reported in most European countries, in the Middle East, USA, China, Canada and Brazil (Fijan et al 1971, Bucke & Finlay 1979, Ahne et al 2002, Goodwin 2002, Dikkeboom et al 2004, Liu et al 2004, Garver et al 2007, Warg et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of spring viraemia of carp virus isolates from Austria indicates the existence of at least two subgroups within genogroup Id

Basic¹,

Schachner²,

Bilić³

et al. 2009

Dis. Aquat. Org.

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Identification of spring viraemia in carp (Cyprinus carpio L) in Great Britain

Abstract: An investigation into a mortality of carp revealed a virus which was identified serologically and morphologically as being identical to SVC virus. SVC is a serious disease of European carp farms and this is the first positive identification of this disease in Great Britain.

Cited by 14 publications

References 0 publications

Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay

Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay

Recent achievements in studies on diseases of common carp (Cyprinus carpio L.)

Phylogenetic analysis of spring viraemia of carp virus isolates from Austria indicates the existence of at least two subgroups within genogroup Id

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