Identification of spermatogenesis-related lncRNA in Holstein bull testis after sexual maturity based on transcriptome analysis

Zhao, Shanjiang; Wang, Huan; Hu, Zhihui; Sahlu, Bahlibi Weldegebriall; Heng, Nuo; Gong, Jianfei; Wang, Haoyu; Zhu, Huabin

doi:10.1016/j.anireprosci.2022.107146

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…It is worth noting that a significant SNP, ARS-BFGL-NGS-37893, was associated with both TCF23 and ABHD1 (Supplementary Table S1). It has been found that these genes are expressed in the testes [49][50][51][52] and may be pivotal in maintaining acrosome integrity after freezing.…”

Section: Absence Of Acrosomesmentioning

confidence: 99%

“…We previously reported that this gene is differentially expressed in the sperm of bulls with different levels of fertility [51]. DPYD is a dihydropyrimidine dehydrogenase that catabolizes pyrimidine nucleotides, precursors for the synthesis of DNA and RNA [52]. Pyrimidine nucleotides are involved in DNA replication and repair during spermatogenesis [53].…”

Section: Swollen Acrosomesmentioning

confidence: 99%

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Risk of Sperm Disorders and Impaired Fertility in Frozen–Thawed Bull Semen: A Genome-Wide Association Study

Dementieva,

Dysin,

Shcherbakov

et al. 2024

Animals

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Cryopreservation is a widely used method of semen conservation in animal breeding programs. This process, however, can have a detrimental effect on sperm quality, especially in terms of its morphology. The resultant sperm disorders raise the risk of reduced sperm fertilizing ability, which poses a serious threat to the long-term efficacy of livestock reproduction and breeding. Understanding the genetic factors underlying these effects is critical for maintaining sperm quality during cryopreservation, and for animal fertility in general. In this regard, we performed a genome-wide association study to identify genomic regions associated with various cryopreservation sperm abnormalities in Holstein cattle, using single nucleotide polymorphism (SNP) markers via a high-density genotyping assay. Our analysis revealed a significant association of specific SNPs and candidate genes with absence of acrosomes, damaged cell necks and tails, as well as wrinkled acrosomes and decreased motility of cryopreserved sperm. As a result, we identified candidate genes such as POU6F2, LPCAT4, DPYD, SLC39A12 and CACNB2, as well as microRNAs (bta-mir-137 and bta-mir-2420) that may play a critical role in sperm morphology and disorders. These findings provide crucial information on the molecular mechanisms underlying acrosome integrity, motility, head abnormalities and damaged cell necks and tails of sperm after cryopreservation. Further studies with larger sample sizes, genome-wide coverage and functional validation are needed to explore causal variants in more detail, thereby elucidating the mechanisms mediating these effects. Overall, our results contribute to the understanding of genetic architecture in cryopreserved semen quality and disorders in bulls, laying the foundation for improved animal reproduction and breeding.

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Section: Absence Of Acrosomesmentioning

confidence: 99%

Section: Swollen Acrosomesmentioning

confidence: 99%

Risk of Sperm Disorders and Impaired Fertility in Frozen–Thawed Bull Semen: A Genome-Wide Association Study

Dementieva,

Dysin,

Shcherbakov

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…They display remarkable specificity for tissues, diseases, and developmental stages. lncRNAs play a crucial role in regulating testicular development and spermatogenesis ( He et al, 2021 ), and changes in their expression levels are linked to the process of testicular development in pigs, cattle, and sheep ( He et al, 2021 ; Chen et al, 2022a ; Zhao et al, 2022 ). Many highly expressed lncRNAs influence sperm development, maturation, and viability within the testis.…”

Section: Introductionmentioning

confidence: 99%

Identification of key genes affecting sperm motility in chicken based on whole-transcriptome sequencing

Guo,

Liu,

et al. 2023

Poultry Science

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Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity

Sahlu,

Wang,

et al. 2023

Animal Reproduction Science

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Identification of spermatogenesis-related lncRNA in Holstein bull testis after sexual maturity based on transcriptome analysis

Cited by 3 publications

References 38 publications

Risk of Sperm Disorders and Impaired Fertility in Frozen–Thawed Bull Semen: A Genome-Wide Association Study

Risk of Sperm Disorders and Impaired Fertility in Frozen–Thawed Bull Semen: A Genome-Wide Association Study

Identification of key genes affecting sperm motility in chicken based on whole-transcriptome sequencing

Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity

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