Identification of sperm antigens as a first step towards the generation of a contraceptive vaccine to decrease fossorial water vole Arvicola terrestris Scherman proliferations

Grignard, Elise; Cadet, Rémi; Saez, Fabrice; Drevet, Joël R.; Vernet, Patrick

doi:10.1016/j.theriogenology.2007.06.010

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…However, species-specific binding of sperm to ZP has potential for developing species-specific immunocontraceptives based on sperm-surface antigens (e.g. Moore et al 1997;Grignard et al 2007;Naz 2011). Recognition of sperm antigens that participate in sperm-ZP binding can be achieved using phage display techniques (Eidne et al 2000;Naz 2005).…”

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

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Abstract. As human populations grow, conflicts with wildlife increase. Concurrently, concerns about the welfare, safety and environmental impacts of conventional lethal methods of wildlife management restrict the options available for conflict mitigation. In parallel, there is increasing interest in using fertility control to manage wildlife. The present review aimed at analysing trends in research on fertility control for wildlife, illustrating developments in fertility-control technologies and delivery methods of fertility-control agents, summarising the conclusions of empirical and theoretical studies of fertility control applied at the population level and offering criteria to guide decisions regarding the suitability of fertility control to mitigate human-wildlife conflicts. The review highlighted a growing interest in fertility control for wildlife, underpinned by increasing numbers of scientific studies. Most current practical applications of fertility control for wild mammals use injectable single-dose immunocontraceptive vaccines mainly aimed at sterilising females, although many of these vaccines are not yet commercially available. One oral avian contraceptive, nicarbazin, is commercially available in some countries. Potential new methods of remote contraceptive delivery include bacterial ghosts, virus-like particles and genetically modified transmissible and non-transmissible organisms, although none of these have yet progressed to field testing. In parallel, new species-specific delivery systems have been developed. The results of population-level studies of fertility control indicated that this approach may increase survival and affect social and spatial behaviour of treated animals, although the effects are species-and context-specific. The present studies suggested that a substantial initial effort is generally required to reduce population growth if fertility control is the sole wildlife management method. However, several empirical and field studies have demonstrated that fertility control, particularly of isolated populations, can be successfully used to limit population growth and reduce human-wildlife conflicts. In parallel, there is growing recognition of the possible synergy between fertility control and disease vaccination to optimise the maintenance of herd immunity in the management of wildlife diseases. The review provides a decision tree that can be used to determine whether fertility control should be employed to resolve specific human-wildlife conflicts. These criteria encompass public consultation, considerations about animal welfare and feasibility, evaluation of population responses, costs and sustainability.

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Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

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“…Animals were sacrificed by cervical dislocation. The time of vaccination was determined based on a proof-of-concept study [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…In this context, immunocontraception studies carried out to date, on different models, used either whole spermatozoa or specific individual sperm antigens. Injection of whole spermatozoa as immunogens was performed in tammar wallabies [ 31 ], mice [ 32 ], rabbits [ 33 , 34 ], and dogs [ 35 ]. However, in these studies, the determination of the sperm antigens triggering the immune response was not conducted, only antibody production and the effects on fertility were described.…”

Section: Introductionmentioning

confidence: 99%

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

Chorfa

Goubely

Henry-Berger

et al. 2021

IJMS

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The cyclical proliferation of the wild fossorial rodent Arvicola terrestris scherman (ATS) is critical in mid-mountain ecosystems of several European countries. Our goal is to develop an immunocontraceptive vaccine to control their fertility, as a sustainable alternative to chemical poisons currently used. Indeed, these chemicals cause the death of ATS predators and animals sharing their ecosystem, and current laws progressively limit their use, making the development of a targeted vaccination strategy an interesting and efficient alternative. In order to identify species-specific sperm antigens, male and female ATS received subcutaneous injections of whole ATS spermatozoa to elicit an immune response. The analysis of the immune sera led to the identification of 120 immunogenic proteins of sperm cells. Of these, 15 were strictly sperm-specific and located in different regions of the male gamete. Some of these antigens are proteins involved in molecular events essential to the reproductive process, such as sperm–egg interaction, acrosomal reaction, or sperm motility. This approach not only identified a panel of immunogenic proteins from ATS sperm cells, but also demonstrated that some of these proteins trigger an immune response in both male and female ATS. These spermatic antigens are good candidates for the development of a contraceptive vaccine.

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GPX5, the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis

Zhang¹,

Chabory²,

Britan³

et al. 2008

Reprod. Fertil. Dev.

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Using various molecular approaches, including reverse transcription-polymerase chain reaction (RT-PCR), rapid amplification of cDNA ends-PCR, sequencing, northern and western blotting, we found that the mouse GPX5 gene gives rise to at least three different transcripts that are not expressed at the same levels in the mouse epididymis. In addition to the major GPX5 transcript, we show that minor GPX5 transcripts exist, arising either from precocious termination of transcription or an alternative splicing event within intron 4 of the 5 exon-encoding GPX5 single copy gene. Furthermore, we demonstrate that variants of the GPX5 protein that are correlated with the shorter GPX5 transcripts can be detected in caput epididymidis protein extracts and that the various GPX5 isoforms are subject to differential post-transcriptional maturation processes in the mouse epididymis that essentially involve the addition of O-glycosyl extensions. Using a sensitive poly-A+ mRNA tissue blot, as well as RT-PCR and northern assays, we further show that in addition to being expressed in the epididymis, the GPX5 gene is also expressed, albeit at lower levels, in other tissues of the male genital tract, including the testis and prostate. Finally, we present evidence suggesting that the GPX5 gene is expressed in a temporally regulated manner during mouse embryonic development.

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Identification of sperm antigens as a first step towards the generation of a contraceptive vaccine to decrease fossorial water vole Arvicola terrestris Scherman proliferations

Cited by 4 publications

References 54 publications

Fertility control to mitigate human–wildlife conflicts: a review

Fertility control to mitigate human–wildlife conflicts: a review

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

GPX5, the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis

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