Identification of specific hemoglobins within individual erythrocytes

Abstract

The identification of specific antibodies for a variety of inherited hemoglobin variants presents the opportunity to develop sensitive immunochemical measurement techniques for such variants. In this study antibodies specific for human hemoglobins A1 and S were isolated from immune rabbit serum. These were labeled with either fluoroscein isothiocyanate or tetramethylrhodamine isothiocyanate. Erythrocytes suspended in agar were incubated with these labeled antibodies and hemoglobins A1, S, and F were identified within individual cells by fluorescence microscopy. This capability offers potential for more specific investigation into heterogenous distribution of specific hemoglobins within an erythroycte or bone marrow cell population and for developing a sensitive technique applicable to antenatal diagnosis of specific hemoglobinopathies.

Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse

“…The phenomenon of intercellular heterogeneity in the levels of a particular protein within single red cells has also been observed in the distribution of fetal hemoglobin (Hb F) in red cells from adult humans. Using either immunological or alkali-denaturation studies, it has been shown that low levels of Hb F are heterogeneously distributed in red cells from normal adult humans (Shepard et al, 1962;Dan" and Hagiwara, 1967;Headings et al, 1975;Boyer et al, 1975). Heterogeneity in the levels of lib F in single erythrocytes has also been observed in individuals heterozygous for Hb S Wood et al, 1975).…”

Μελετη Του Αντιγονικου Συστηματος Ι-I Των Ερυθροκυτταρων Του Ανθρωπου

“…Μετά άπό λύση τοϋ κυττάρου, τό αντιγόνο διαχέεται κυκλικά καί δημιουρ γεί κατακρημνίσματα, πού μπορούν νά ανιχνευθούν μέ μικροσκοπία σκοτεινού πεδίου. 'Εάν τά αντισώματα είναι συνδεδεμένα μέ φθορί ζουσα ουσία τά κατακρημνίσματα είναι ορατά καί μέ φθορίζουσα μικροσκοπία(52). *Η μελέτη των παρασκευασμάτων γίνεται μέ μικροσκόπιο φθορι σμού τύπου Zeiss μέ σύστημα φωτισμού εκ των άνω καί φίλτρα κα τάλληλα γιά φθορεσκεϊνη ή ροδαμίνη.…”