Identification of Some Organic Acids by Paper Chromatography

Jones, Allan R.; Dowling, E. J.; Skraba, Walter J.

doi:10.1021/ac60075a004

Cited by 93 publications

(16 citation statements)

References 12 publications

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“…Acid substances were located with bromophenol blue indicator. Acetic acid, separated by distillation, was identified on the basis of mobility on paper in a solvent system (Jones, Dowling & Skraba, 1953) containing butanolwater-diethylamine (100: 15: 1, by vol. ).…”

Section: Methodsmentioning

confidence: 99%

Synthesis of cellulose by Acetobacter xylinum. 3. Substrates and inhibitors

Schramm

Gromet

Hestrin

1957

Biochemical Journal

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STRUCTURE OF SERRATAMIC ACID 669 obtained was applied to a paper along with L-serine and authentic a-alanine and the chromatogram run in 80% phenol. Development with ninhydrin showed the product to have the same RF value as a-a]anine. 2:3-Epoxydecanoic acid. Freshly distilled octyl aldehyde (5.7 g.) and ethyl chloroacetate (5.5 g.) in dry ether (100 ml.) were added to sodium methoxide (from 10 g. of Na) suspended in dry ether. After standing for 48 hr. at room temp. water was added, and the ether layer was separated and dried over anhydrous Na2SO4. The oil left after removal of the solvent was purified by vacuum distillation, yielding ethyl 2:3-epoxydecanoate (6 g., b.p. 90-93o/0.1 mm.). Saponification rapidly occurred on the addition of an excess of 12% (w/v) KOH in methanol at room temp., with the * Part 2: Hestrin & Schramm (1954). For a preliminary communication on someexperimentsdescribedinthepresent paper and results obtained in a tracer analysis of cellulose production, see Schramm, Gromet & Hestrin (1957).

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Section: Methodsmentioning

confidence: 99%

Synthesis of cellulose by Acetobacter xylinum. 3. Substrates and inhibitors

Schramm

Gromet

Hestrin

1957

Biochemical Journal

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“…The appropriate fractions were combined and the separated aqueous phase was treated with charcoal (Norit Ultra, United Norit Sales Corp. Ltd., Amsterdam) to remove the indicator. After acidification the organic acid was isolated by continuous extraction with diethyl ether and its identity was confirmed by paper chromatography (Jones, Dowling & Skraba, 1953) with the spray reagent containing glucose and aniline that was described by Carles, Schneider & Lacoste (1958). (b) Phosphate esters.…”

Section: Methodsmentioning

confidence: 99%

The metabolism of Plasmodium berghei, the malaria parasite of rodents. 2. An effect of mepacrine on the metabolism of glucose by the parasite separated from its host cell

Bowman

Grant

Kermack

et al. 1961

Biochemical Journal

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“…In some cases, after growth for 8-9 days the culture fluids were extracted continuously with ether; the material extracted in this way was shown to have the same R, value as authentic kojic acid on paper chromatography in two solvent systems. R, of kojic acid in butanol-acetic acid-water (4:1:5, v/v) = 0-66; in 2-ethylbutan-1-ol-5M formic acid (2:3, v/v) = 0-37 (system of Jones, Dowling & Skraba, 1953). The dried papers were sprayed with 1 % ferric chloride in 0O-N-HCI to locate the kojic acid.…”

Section: Methodsmentioning

confidence: 99%