Identification of Some Allergenic Pollen Proteins of Some Fabaceae and Poaceae Species using SDS-PAGE Technique

Abstract

The current study aims to identify the allergenic pollen proteins of some plant species of Fabaceae and Poaceae families by SDS-PAGE technique to know which of them can cause allergy. Six different plants belonging to Fabaceae family (Bauhinia variegate, Cassia javanica, Delonix regia, Peltophorumafricanum, Senna didymobotrya and Senna surattensis) and four different plants belonging to Poaceae family (Avena sativa, Setaria viridis, Sorghum bicolor and Zea mays) were collected from two different zones. The 1 st site is located at Cairo Governorate and the 2 nd is Kafr Elsheikh Governorate. Sampling was carried out at both two mentioned fixed sites for three consecutive periods from July 2018 to June 2021.The obtained results showedthat the prolate-spheroidal was the most common pollen shape, trizonocolpoate and monoporate shapes were the most prevalent aperture and the regulate-perforate and granulate types were the most noticed sculpture. SDS showed notable bands, ranged from 25, to 75 kDa which were related to hydrophilic proteins antigen in pollens. Interestingly, SDS-Page of the serum of the patient revealed similar bands within 74, 51 and 40 kDa. Therefore, our study highlighted the possible role of pollen grains related to Fabaceae and Poaceae families in allergic patient in Egypt.