Identification of Single Specimens of the Anopheles Gambiae Complex by the Polymerase Chain Reaction

Scott, Julie A.; Brogdon, William G.; Collins, Frank H.

doi:10.4269/ajtmh.1993.49.520

Cited by 1,400 publications

(1,249 citation statements)

References 0 publications

Supporting

Mentioning

1,219

Contrasting

Unclassified

Order By: Relevance

“…could be An. merus as the protocol by Scott et al (1993) used in this study did not include primers to identify An. merus which Masendu et al (2005) reported as being common in some parts of Zimbabwe.…”

Section: Resultsmentioning

confidence: 99%

Malaria vector species composition and relative abundance in Mutare and Mutasa districts, Zimbabwe

Sande

Zimba

Chinwada

et al. 2015

J Entomol Acarol Res

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Malaria vector species composition and relative abundance in Mutare and Mutasa districts, Zimbabwe

Sande

Zimba

Chinwada

et al. 2015

J Entomol Acarol Res

View full text Add to dashboard Cite

show abstract

“…Adults were assigned to members of the An. gambiae cryptic‐species complex on the basis of morphological tests and molecular analyses (Gillies & Coetzee, 1987; Santolamazza et al., 2008; Scott, Brogdon, & Collins, 1993). …”

Section: Methodsmentioning

confidence: 99%

Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae

Assogba

Alout

Koffi

et al. 2018

Evolutionary Applications

View full text Add to dashboard Cite

While gene copy‐number variations play major roles in long‐term evolution, their early dynamics remains largely unknown. However, examples of their role in short‐term adaptation are accumulating: identical repetitions of a locus (homogeneous duplications) can provide a quantitative advantage, while the association of differing alleles (heterogeneous duplications) allows carrying two functions simultaneously. Such duplications often result from rearrangements of sometimes relatively large chromosome fragments, and even when adaptive, they can be associated with deleterious side effects that should, however, be reduced by subsequent evolution. Here, we took advantage of the unique model provided by the malaria mosquito Anopheles gambiae s.l. to investigate the early evolution of several duplications, heterogeneous and homogeneous, segregating in natural populations from West Africa. These duplications encompass ~200 kb and 11 genes, including the adaptive insecticide resistance ace‐1 locus. Through the survey of several populations from three countries over 3–4 years, we showed that an internal deletion of all coamplified genes except ace‐1 is currently spreading in West Africa and introgressing from An. gambiae s.s. to An. coluzzii. Both observations provide evidences of its selection, most likely due to reducing the gene‐dosage disturbances caused by the excessive copies of the nonadaptive genes. Our study thus provides a unique example of the early adaptive trajectory of duplications and underlines the role of the environmental conditions (insecticide treatment practices and species ecology). It also emphasizes the striking diversity of adaptive responses in these mosquitoes and reveals a worrisome process of resistance/cost trade‐off evolution that could impact the control of malaria vectors in Africa.

show abstract

“…were processed for species determination using PCR 32 and P falciparum infection using an enzyme-linked immunosorbent assay 33 . Wild caught A gambiae s.l.…”

Section: Entomological Collectionsmentioning

confidence: 99%

Efficacy of indoor residual spraying with dichlorodiphenyltrichloroethane against malaria in Gambian communities with high usage of long-lasting insecticidal mosquito nets: a cluster-randomised controlled trial

Pinder

Jawara

Jarju³

et al. 2015

The Lancet

121

View full text Add to dashboard Cite

(2015) 'E cacy of indoor residual spraying with dichlorodiphenyltrichloroethane against malaria in Gambian communities with high usage of long-lasting insecticidal mosquito nets : a cluster-randomised controlled trial.', The lancet., 385 (9976). pp. 1436-1446. Further information on publisher's website:http://dx.doi.org/10.1016/S0140-6736(14)61007-2 Publisher's copyright statement: NOTICE: this is the author's version of a work that was accepted for publication in The Lancet. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be re ected in this document. Changes may have been made to this work since it was submitted for publication. A de nitive version was subsequently published in The Lancet, 385, 9976, 11 17 April 2015, 10.101661007-2.Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

show abstract

Identification of Single Specimens of the Anopheles Gambiae Complex by the Polymerase Chain Reaction

Cited by 1,400 publications

References 0 publications

Malaria vector species composition and relative abundance in Mutare and Mutasa districts, Zimbabwe

Malaria vector species composition and relative abundance in Mutare and Mutasa districts, Zimbabwe

Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae

Efficacy of indoor residual spraying with dichlorodiphenyltrichloroethane against malaria in Gambian communities with high usage of long-lasting insecticidal mosquito nets: a cluster-randomised controlled trial

Contact Info

Product

Resources

About