Identification of signal pathways for immunotoxicity in the spleen of common carp exposed to chlorpyrifos

Xing, Houjuan; Teng, Xiaohua; Peng, Muqiao; Wang, Zhilei; Liu, Feng; Li, Shu

doi:10.1016/j.ecoenv.2019.109464

Cited by 37 publications

(10 citation statements)

References 62 publications

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“…Improving our knowledge of the potential impacts of chemical pollutants on wildlife is an important aspect of biological conservation. Unfortunately, traditional methods of obtaining samples in ecotoxicology are invasive (Jasinska et al 2015, Wilkie et al 2018, Boisvert et al 2019, Xing et al 2019, da Costa Araujo et al 2020, and research on non-destructive methods is severely lacking (Chaousis et al 2018). Nevertheless, non-invasive methods have already been applied in several ecotoxicology studies.…”

Section: Ecotoxicologymentioning

confidence: 99%

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Zemanova

2020

Wildlife Biology

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Section: Ecotoxicologymentioning

confidence: 99%

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Zemanova

2020

Wildlife Biology

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“…Twelve pathways belonging to five major categories (environmental information processing, cellular processes, organismal systems, human diseases, and not included in pathway or brite) were more abundant in wild tigers. A large part of these functions was related to human diseases that have essentially influenced by environmental factors (Kanehisa et al, 2009) or organism systems and played an important role in ensuring the normal function of the host and maintaining a stable state (Xing et al, 2019). These pathways are more prevalent in wild tigers, possibly because they faced more diverse conditions in the wild than in captive tigers, such as expanding their territory and home range and hunting various prey to obtain energy supply.…”

Section: Differences In Gut Microbiota Functions Between Wild and Capmentioning

confidence: 99%

Comparative Analysis of Microbial Community Structure and Function in the Gut of Wild and Captive Amur Tiger

Yao

Dobbins

et al. 2020

Front. Microbiol.

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It has been well acknowledged that the gut microbiome is important for host health, composition changes in these microbial communities might increase susceptibility to infections and reduce adaptability to environment. Reintroduction, as an effective strategy for wild population recovery and genetic diversity maintenance for endangered populations, usually takes captive populations as rewilding resource. While, little is known about the compositional and functional differences of gut microbiota between captive and wild populations, especially for large carnivores, like Amur tiger. In this study, high throughput sequencing of the 16S ribosomal RNA (rRNA) gene (amplicon sequencing) and metagenomics were used to analyze the composition and function variations of gut microbiota communities between captive and wild Amur tiger populations based on total 35 fecal samples (13 from captive tigers and 22 from wild tigers). Our results showed that captive Amur tigers have higher alpha diversity in gut microbiota, but that the average unweighted UniFrac distance of bacterial taxa among wild Amur tigers was much larger. The function differences involve most aspects of the body functions, especially for metabolism, environmental information processing, cellular processes, and organismal systems. It was indicated that the diet habit and environment difference between captive and wild populations lead to composition differences of gut microbiota and then resulted in significant differences in functions. These contrasts of functional and compositional variations in gut microbiota between wild and captive Amur tigers are essential insights for guiding conservation management and policy decision-making, and call for more attention on the influence of gut microbiota on the ability of captive animals to survive in the wild.

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“…Lymphocytes are a kind of cell line with immune recognition function and act as a "soldier" who fight against external infections and monitor cell variability in the body. The ways to eliminate pathogens of lymphocytes include direct killing, cellular immunity and humoral immunity (Xing et al, ). T cells are the core of the body's cellular immunity throughout the immune system.…”

Section: Introductionmentioning

confidence: 99%

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Yang

Gong

Cai

et al. 2020

Journal of Fish Diseases

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Chlorpyrifos is a poisonous pesticide that is highly toxic to fish and aquatic organisms. However, there are fewer reports about how chlorpyrifos influences the redox balance of immune cells. Herein, the head kidney tissue treated with chlorpyrifos to do transcriptome analysis and TCR γ was screened out. Subsequently, we established TCR γ knockdown and overexpression carp head kidney lymphocyte models, respectively, by using RNA interference and pcDNA3.1. Real‐time PCR, fluorescent staining, oxidation and antioxidant kit were used to detect the related factors. We found that TCR γ knockdown significantly increased the mRNA expression of HSP70 and HSP90 and decreased the mRNA expression of SOD and CAT. Meanwhile, TCR γ knockdown reduced the activities of GSH, GSG‐PX, T‐AOC, CAT and SOD and increased the content of MDA and H2O2 and activities of iNOS. Adverse results were obtained in TCR γ overexpression group. Additionally, TCR γ knockdown significantly increased the mRNA expression of IFN‐γ, IL‐1β, IL‐8, IL‐10, Nrf2 and NF‐κB, but relieved TCR γ overexpression, in which the process of inflammation was activated. Our results reported here indicated that chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ, and HSPs play potential protective role in entire process.

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Identification of signal pathways for immunotoxicity in the spleen of common carp exposed to chlorpyrifos

Cited by 37 publications

References 62 publications

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Comparative Analysis of Microbial Community Structure and Function in the Gut of Wild and Captive Amur Tiger

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

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