Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice

Mahmoud, Magdy S.; Rehan, Ibrahim F.; Ahmed, Kh El-Dawy; Abdelrahman, Amany; Mohammadi, Saeed; Abou-Elnaga, Ahmed F.; Youssef, Mohamed Ben; Diab, Hassan Mahmoud; Salman, Doaa; Elnagar, Ashraf; Mohammed, Hesham H.; Shanab, Obeid; Ibrahim, R.M. Al; Ahmed, Eslam; Hesham, Abd El‐Latif; Gupta, Arti

doi:10.1007/s11033-019-04717-7

Cited by 9 publications

(16 citation statements)

References 60 publications

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“…S3, in Supplementary Information, for experiment III). Our result agreed with our previous study reported and revealed a failure of homeostatic immune balance after exposure to chronic unpredictable mild stressors in BALB/c mice 18 . Thus, we have significantly discovered N-glycan expression in adaptive immunity due to the dynamic infection variations.…”

Section: Neu5gc/neu5ac Ratio Of Toxoplasmosis the Changing Of Neu5gcsupporting

confidence: 94%

“…Data represent as mean ± SD. serum glycomics protocols 18,20,24 . The expression of the two principles sialic acid(s) (Neu5Ac-, Neu5Gc-terminal) synthesized by non-mammal cells in glycoproteins might be beneficial for assessing modifications in the regulation of homeostasis, animal behavior, and analysis of N-glycomes of BALB/c mice sera.…”

Section: Maldi-tof/ms-analysis Of N-glycans (Experiments I-iii) Diagrmentioning

confidence: 99%

“…Glycoblotting was carried out using mice sera (n= 288) in order to quantify N-glycomes. Whole N-glycomics were analyzed as described before with minor modulations 18,20,24,61 . Mouse sera glycoproteins were achieved in order to purify the entire N-glycans using PNGase F enzyme.…”

Section: Parasite and Infection With T Gondii T Gondii Type-ii (Plmentioning

confidence: 99%

“…Importantly, N-glycosylation is the first line of defense to control the function of immune system 13 . In addition, N-glycosylation has a potential effect on the other neuropsychiatric disorders like post-traumatic stress disorder 16 , schizophrenia 17 , and stress-induced depressive behaviors in mice 18 . Moreover, we recorded the inability of the homeostatic immune system of BALAB/c mice to cope the chronic stressors.…”

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confidence: 99%

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Sialylated N-glycan profile during acute and chronic infections with Toxoplasma gondii in mice

Rehan

Mahmoud

Salman

et al. 2020

Sci Rep

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Toxoplasma gondii is associated with physiological and psychiatric perturbations. The immune response is interrelated to the progress of anhedonia and despair symptoms of T. gondii-infected subjects. We recently reported that serum N-glycans were altered in mice displayed depressive-like behaviors. However, a novel biomarker that correlated to T. gondii infection and associated behaviors is demanded. Glycomics has been used to find affected glycoproteins during depression. The objective of this study is to investigate serum N-glycomics changes during infection with T. gondii in BALB/c mice, immunocompetent, or in severe combined immunodeficient mice, and after treatment with an immunostimulant; 1-methyl tryptophan. Glycans were examined through glycoblotting-protocol then investigated by MALDI-TOF/MS. Both depressive and sickness-related behaviors were significantly abundant (P ≤ 0.001 each), during acute T. gondii in immunocompetent mice, compared to controls. Only sickness symptoms were evident in immunodeficient mice infected with T. gondii, as associated with high expression level (P ≤ 0.001) of Peak # 15 (2 × Neu5Gc) compared to controls. The alteration of sialylated N-glycan expressions is important to detect the immune status of animals/humans against T. gondii. Moreover, 1-methyl tryptophan reduced depressive-like behavior (P ≤ 0.001) compared to controls. Therefore, sialylated N-glycan (Neu5Ac/Neu5Gc-terminal) is targeted to be used as a novel biomarker of sickness/depressive-like behaviors. Toxoplasma gondii affects all warm-blooded animals and infects almost one-third of all humans. T. gondii infection is a parasitic disease prevalent worldwide and involved in many psychiatric disorders 1. Moreover; depression is considered as one of the major psychological disorders and the global root of disability in the 21 st century 2. It is defined as a mood disorder due to the reduction of interest, happiness, learning abilities, sleep, appetite, and energy 3. Several studies had shown that seroprevalence of T. gondii in neuropsychiatric defects were variable like schizophrenia, bipolar mood disorder 4 and self-directed violence 5. Challenge with T. gondii revealed two characteristic forms: immune-stimulating tachyzoites and immune-encrypted bradyzoites. Recent studies had demonstrated that the brain is an immune-privileged region of residence of bradyzoite cysts 6. Hence, the pathogenesis of infection depends mainly upon host immunity activated macrophages and lymphocytes immediately primed to kill intracellular tachyzoites 7. However, under lowered immunity, the propagation of tachyzoites can be quite high as shown in immune-competent individuals 8 , which may result in fatal toxoplasmic encephalitis 9. T. gondii

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Section: Neu5gc/neu5ac Ratio Of Toxoplasmosis the Changing Of Neu5gcsupporting

confidence: 94%

Section: Maldi-tof/ms-analysis Of N-glycans (Experiments I-iii) Diagrmentioning

confidence: 99%

Section: Parasite and Infection With T Gondii T Gondii Type-ii (Plmentioning

confidence: 99%

mentioning

confidence: 99%

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Sialylated N-glycan profile during acute and chronic infections with Toxoplasma gondii in mice

Rehan

Mahmoud

Salman

et al. 2020

Sci Rep

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“…Gokul et al [50] found increased serum glutamic-oxaloacetic transaminase, pyruvic transaminase, MDA, corticosterone and total antioxidant level in Wistar rats exposed to chronic unpredictable mild stress (CUMS) that developed comorbid depression accompanied by an inflammatory response. A different, glycomicscentered study of CUMS in BALB/c mice revealed altered N-glycan patterns of serum glycome [51]. Specifically, a potential biomarker of CUMS-induced depression was reported to be the disialylated glycans (A2G2S2 and A2G2Z2) ratio, signalizing changes in sialic acids expression.…”

Section: Chronic Stress Induces Subclass-specific Changes In Rat Igg Glycosylationmentioning

confidence: 99%

High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress

Habazin

Mlinarević

Balog

et al. 2021

Journal of Proteomics

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Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex-and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response. Significance: In this study, we showed that a high-throughput streamlined methodology based on protein L 96well monolithic plates for efficient rat IgG immunoaffinity enrichment from blood plasma, paired with appropriate tryptic glycopeptide preparation, HILIC-SPE enrichment, and nano-LC-MS methods was suitable for quick processing of large sample sets. We report a subclass-specific profiling and changes in rat IgG Fc galactosylation and adrenal gland immunohistochemistry of male and female animals exposed to a customized chronic stress protocol.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Harvey

2023

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. The review is basically divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of arrays. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other areas such as medicine, industrial processes and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. The reported work shows increasing use of incorporation of new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented nearly 40 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show little sign of diminishing.

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Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice

Cited by 9 publications

References 60 publications

Sialylated N-glycan profile during acute and chronic infections with Toxoplasma gondii in mice

Sialylated N-glycan profile during acute and chronic infections with Toxoplasma gondii in mice

High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

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