Identification of serum biomarkers for ovarian cancer using MALDI–TOF-MS combined with magnetic beads

Abstract

The results suggested that biomarkers for ovarian cancer diagnosis in serum could be identified by MALDI-TOF-MS combined with the use of magnetic beads. The use of combined biomarkers would further enable powerful and reliable diagnosis of ovarian cancer with high sensitivity and specificity.

MALDI-TOF-MS analysis in discovery and identification of serum proteomic patterns of ovarian cancer

“…Complement C3, inter-alpha-trypsin inhibitor heavy chain H4 and transthyretin were also identified using a combination of carrier protein-bound affinity enrichment strategy with MALDI-TOF MS to characterize OC samples, which is in agreement with our findings [24]. Unfortunately, other OC studies based on MALDI-TOF MS profiling lack the identification step [25, 26]. It is unknown whether other depletion methods are capable of detecting relevant proteins.…”

“…In the literature, there are several studies focusing on plasma and serum proteomic patterns of ovarian cancer obtained by MALDI-TOF MS. In order to detect low abundance proteins and peptides, biomarker enrichment kits [24], immunodepletion [25] and magnetic beads [26] have been applied in OC studies. Due to significant impact of sample pretreatment on the MS spectra, the discriminative peaks proposed as candidates for OC biomarkers depend on efficiency of the enrichment strategy.…”

“…Protein-peptide profiling studies based on MALDI-TOF MS analyses are often limited to a list of the most discriminative m/z peaks as potential disease indicators [15, 25, 26]. Nevertheless, the identification step is crucial for understanding the pathological processes that occur during cancer development and it should not be omitted.…”

Multiplexed magnetic nanoparticle-antibody conjugates (MNPs-ABS) based prognostic detection of ovarian cancer biomarkers, CA-125, β-2M and ApoA1 using fluorescence spectroscopy with comparison of surface plasmon resonance (SPR) analysis

“…Screening and analysis of serum biomarkers is convenient in general compared to tissue specific as there is no need of biopsy or any other surgical methods. To analyze and quantify the ovarian cancer serum biomarkers, many methods have been used to detect the ovarian cancer at early stage such as; Quartz crystal microbalance (Sania et al, 2015;Jing et al, 2005), Mass spectroscopy (Charlotte et al, 2011;Wu et al, 2012;Soper et al, 2006;Ying et al, 2010), Immune assay (Archana et al, 2012;Shoumei et al, 2012;Siriwan et al, 2009;Zhifeng et al, 2008;Jesse et al, 2009), Electrochemical (Israa et al, 2013;He et al, 2013;Subramanian et al, 2012), surface Plasmon resonance (Brandon et al, 2011). Screening and quantification of serum biomarkers with routine medical examination methods enhanced the detection methodology of ovarian cancer at early stage.…”

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

“…Studies of urine proteome give important insight into health status of the body. The obtained results can be used in diagnostics and treatment of various diseases, such as bladder cancer, ovarian cancer, progressive glomerular disease, renal cell cancer or gastric cancer (D'Amico et al 2003, Khan et al 2005, Holt et al 2006, Shigaki et al 2006, Salo et al 2007, Yang et al 2009, Saito et al 2010, Welton et al 2010, Jansen et al 2011, Nabity and Lees 2012, Wu et al 2012. High sensitivity and resolution of available analytical methods allows listing the proteins present in the urine including those at low concentrations and making a map for each pathological state (Anderson et al 1979).…”