Identification of serotypes and virulence markers of Escherichia coli isolated from human stool and urine samples in Egypt

Osman, Kamelia M.; Mustafa, A. M.; Elhariri, Mahmoud; AbdElhamed, GS

doi:10.4103/0255-0857.99492

Cited by 17 publications

(15 citation statements)

References 18 publications

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“…MDRs were reported at high rates for E. coli (87.5%) and S. aureus (95.5%) isolates in this study. In accordance with our findings, high MDR rates of these two pathogens in milk and dairy products were reported in several countries including Egypt [11,12,25,28,31]. The highest resistance rates of E. coli isolates were for cephalothin, which agreed with findings of Paneto et al [27].…”

Section: Discussionsupporting

confidence: 92%

“…O158-STEC and O125-STEC were previously reported from cattle samples in Egypt [11] and elsewhere [27]. All detected STEC serogroups detected in this study were implicated in human diseases as follow; O157-STEC in cases of Diarrhea in Egypt [11], O126-STEC, O157-STEC and O158-STEC in urinary tract infection cases in Egypt [11,28], while O125-STEC and O146-STEC were detected in human diarrhea cases from elsewhere [29]. ETEC were isolated from 1.7% of milk samples.…”

Section: Discussionsupporting

confidence: 67%

“…O126-ETEC and O167-ETEC were associated with cases of human diarrhea in Egypt [10] and worldwide [8]. Moreover, O126-ETEC was isolated from more than 30% of human urine samples in Egypt [28].…”

Section: Discussionmentioning

confidence: 99%

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Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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Introduction: Milk is an important food in Egypt and most of it is sold as raw milk in informal markets. Methodology: This study was conducted to investigate the public health risks of Escherichia coli and Staphylococcus aureus in milk sold in informal markets in Egypt. A total of 121 milk samples were analyzed for occurrence, virulence genes and antibiotic resistance of E. coli and S. aureus. Results: A total of 35/121 (28.9%) of milk samples were contaminated with 16/121 (13.2%) E. coli, 22/121 (18.2%) S. aureus, and 3/121 (2.5%) both isolates. Shiga-toxin producing E. coli (STEC), Enterotoxigenic E. coli (ETEC) and Enterotoxigenic S. aureus were detected in 5/121 (4.1%), 2/121 (1.7%) and 8/121 (6.6%) of the examined milk samples, respectively. Multiple drug resistances (MDRs) were showed by 14/16 (87.5%) and 21/22 (95.5%) of E. coli and S. aureus isolates, respectively. E. coli isolates showed high resistance for cephalothin (87.5%), ampicillin (68.8%) and tetracycline (68.8%), but were sensitive for gentamicin and chloramphenicol. Resistance phenotypes of E. coli were diverse; however, STEC isolates were significantly associated with co-resistance to cephalothin, ampicillin and tetracycline (P< 0.05). Two (9.1%) of S. aureus isolates were methicillin-resistant (MRSA) but sensitive to gentamicin (GS-MRSA). Five (22.7%) of S. aureus isolates were gentamicin-resistant methicillin-sensitive S. aureus (GR-MSSA). S. aureus isolates also showed high resistance for ampicillin (100%), tetracycline (90.1%) and sulfamethoxazole-trimethoprim (90.1%). Conclusion: These findings highlighted the potential public health hazards of E. coli and S. aureus pathogens in raw milk sold in informal markets in Egypt.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 67%

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Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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“…Binding of congo red dye by E. coli is associated with the pathogenicity of the organism and can be used as a phenotypic marker to distinguish invasive and non-invasive isolates of E. coli Vinal, 1986 andOsman et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Some virulence factors of Escherichia coli isolated from diarrheic calves

Osman

Hassan

Azzam

et al. 2018

Journal of Veterinary Medical Research

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Escherichia coliare the most common cause of diarrhea in calves. Diarrhea in calvesremains one of the most important problems faced by livestock, causing great economic losses. Some strains ofE. coli characterized by the presence of specific virulence factors including haemolysin production, resistance to bactericidal effects of serum and Congo red binding activity. In this studyfecal samples were collected from 115 diarrheic calves aged from 3 days to one year and from different localities in Egypt along the period from February to August 2015.The prevalence of E. coli in diarrheic calves was 72.2%. 39.8% of isolated E. coli were haemolytic to sheep blood agar, 68.7% were serum resistant, 100% showed Congo red binding activity.

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“…Among the six recognized diarrhoeagenic categories of E. coli ETEC is the most common, particularly in the developing world (Osman et al, 2012). Enterotoxigenic E. coli (ETEC) is a major pathogen of animals, being responsible for diarrhea in calves resulting significant financial losses (Zinnah et al, 2007) and the major enteropathogen associated with neonatal calf diarrhoea worldwide (Moxley and Smith ,2010).…”

Section: Page2mentioning

confidence: 99%

Isolation and Molecular Characterization of ETEC and NTEC Escherichia coli from Cattle Farm With Reference to Virulence Marker Gene

Warke¹,

Bobade²,

Ingle³

2017

Int. J. Livest. Res.

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Identification of serotypes and virulence markers of Escherichia coli isolated from human stool and urine samples in Egypt

Abstract: This is the first report on the molecular characterization of E. coli diarrhoeagenic strains in Egypt and the first report on the potential role of E. coli in diarrhoea and urinary tract infections in a localized geographic area where the people engage in various occupational activities.

Cited by 17 publications

References 18 publications

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Some virulence factors of Escherichia coli isolated from diarrheic calves

Isolation and Molecular Characterization of ETEC and NTEC Escherichia coli from Cattle Farm With Reference to Virulence Marker Gene

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