Identification of selenocompounds in yeast by electrospray quadrupole-time of flight mass spectrometry

McSheehy, Shona; Szpunar, Joanna; Haldys, Violette; Tortajada, Jeanine

doi:10.1039/b201015c

Cited by 94 publications

(104 citation statements)

References 21 publications

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“…This problem can be partly alleviated by careful optimization of the separation conditions [5,6] or by running the same sample with different principle separation techniques, in parallel [7] or in sequence [8,9], was also reported.…”

Section: Introductionmentioning

confidence: 93%

Multidimensional liquid chromatography with parallel ICP MS and electrospray MS/MS detection as a tool for the characterization of arsenic species in algae

et al. 2001

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An analytical strategy was developed for the characterization of arsenic species in a Laminaria algae. The approach was based on multidimensional liquid chromatography (LC) including sample extract cleanup by size-exclusion LC, separation of arsenic species by anion-exchange LC, verification of the chromatographic purity of arsenic-containing fractions, and their further purification, if necessary, by reversed-phase (RP) HPLC. The complementarity of ICP MS, used as the chromatographic detector, and ES MS/MS, employed for the identification of the peaks observed, was demonstrated. The species found were: arsenosugar A 11.7+/-0.5 microg g(-1), AsV 10.9+/-2.1 microg g(-1), arsenosugar B 2.22+/-0.07 microg g(-1), arsenosugar D 1.5+/-1.2 microg g(-1), a newly detected arsenosugar 1.13+/-0.07 microg g(-1), arsenosugar C 0.61+/-0.04 microg g(-1), DMA 0.42+/-0.02 microg g(-1) and these accounted for >99% of the arsenic present. The identities of all the species, except the newly detected compound, were doubly checked by matching the retention times of chromatographically pure (after the 3rd LC dimension) species with standards and by ES MS/MS.

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Section: Introductionmentioning

confidence: 93%

Multidimensional liquid chromatography with parallel ICP MS and electrospray MS/MS detection as a tool for the characterization of arsenic species in algae

et al. 2001

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“…12 These kinds of sample have been investigated with a variety of analytical techniques in order to establish selenium species distribution and possible pathways during metabolism. These methods frequently involve enzymatic hydrolysis, 13,14 followed by high-performance liquid chromatography (HPLC) or ion-exchange separation 15 and mass spectrometric detection, using either electrospray ionization (ESI) 16 or ICP 17 as ionization or excitation source respectively. The principal selenium compounds in these yeast extracts were identified as Se-Met and Se-adenosyl-selenohomocysteine, 18 accounting for about 85% of the total selenium content in yeast samples.…”

Section: Introductionmentioning

confidence: 99%

Volatile organoselenium monitoring in production and gastric digestion processes of selenized yeast by solid‐phase microextraction‐multicapillary gas chromatography coupled microwave‐induced plasma atomic emission spectrometry

Sanz‐Landaluze

Dietz

Madrid

et al. 2004

Applied Organom Chemis

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Evolution of volatile organoselenium compounds in the production and gastric digestion of selenized yeast has been monitored. The industrial production of these kinds of material, employed as food supplements, has been simulated in a process of yeast enrichment with inorganic selenium selenium (IV) in different growth media, with variation of the pH value. The in vitro gastric digestion process was carried out with pepsin in an acid and salt mixture. Determination of volatile species of selenium was achieved coupling solid-phase microextraction (SPME) for preconcentration and sample-matrix separation and microwave-induced plasma atomic emission spectrometry, in combination with multicapillary (MC) gas chromatography for separation and detection of the selenium species. The MC column was operated at low temperatures (∼30 • C). The method was optimized, using a chemometric approach, with respect to the detection of organoselenium species such as dimethylselenide, diethylselenide and dimethyldiselenide. SPME sampling was carried out in the headspace above the corresponding solutions. Separation is fast, with a chromatogram being obtained in less than 5 min, and the detection limits were at the low parts per billion level for all species investigated. The results of the yeast enrichment process demonstrate inorganic selenium transformation into volatile organic species. The presence of inorganic selenium gave rise to at least five different volatile species after metabolization by yeast, with dimethylselenide and dimethyldiselenide being the predominant species. Commercial pasteurized yeast, containing mainly selenomethionine for use as a food supplement, and tablets were found to be still active under conditions of the simulation of the digestion process, even though producing relatively low amounts of organoselenium compounds.

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“…A recent identification of low molecular selenocompounds in yeast 83 and an investigation of the fragmentation of cyclic peptides belonging to the polymyxin and colistin antibiotics 87 can serve as examples of the application of a tandem ESI-Q-TOF-MS system.…”

Section: An Overview Of Analytical Applications Of Tof-ms Analyzersmentioning

confidence: 99%

“…The analysis of human hemoglobin by using a hybrid microchip nanospray device and TOF-MS has been described. 79 Inorganic and organic antimony compounds have been investigated by ESI-TOF-MS. 81 Electrospray TOF-MS has been found to be an excellent detector (analysis in the low femtomol range) for fast liquid chromatography 77,82,83 and capillary electrophoresis 70,71,[73][74][75][76][77]80 used for the identification and quantification of low and high molecular-weight species. The combination of electrospray-TOF-MS with on-line liquid chromatography for estimating the precision of the exact mass 84 Soon after the development of ESI-TOF-MS, a tandem (hybrid) technique incorporating a quadrupole as a first mass analyzer and orthogonal TOF-MS to record the product ions was developed.…”

Section: An Overview Of Analytical Applications Of Tof-ms Analyzersmentioning

confidence: 99%

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

Balcerzak

2003

ANAL. SCI.

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Identification of selenocompounds in yeast by electrospray quadrupole-time of flight mass spectrometry

Cited by 94 publications

References 21 publications

Multidimensional liquid chromatography with parallel ICP MS and electrospray MS/MS detection as a tool for the characterization of arsenic species in algae

Multidimensional liquid chromatography with parallel ICP MS and electrospray MS/MS detection as a tool for the characterization of arsenic species in algae

Volatile organoselenium monitoring in production and gastric digestion processes of selenized yeast by solid‐phase microextraction‐multicapillary gas chromatography coupled microwave‐induced plasma atomic emission spectrometry

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

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