Identification of NADPH oxidase family members associated with cold stress in strawberry

Abstract

NADPH oxidase is encoded by a small gene family (Respiratory burst oxidase homologs, Rbohs) and plays an important role in regulating various biological processes. However, little information about this gene family is currently available for strawberry. In this study, a total of seven Rboh genes were identified from strawberry through genomewide analysis. Gene structure analysis showed the number of exons ranged from 10 to 23, implying that this variation occurred in FvRboh genes by the insertion and distribution of introns; the order and approximate size of exons were relatively conserved. FvRbohC was predicted to localize to the thylakoid membrane of the chloroplast, while other members were computed to localize to the plasma membrane, indicating different functions. Amino acid sequence alignment, conserved domain, and motif analysis showed that all identified FvRbohs had typical features of plant Rbohs. Phylogenetic analysis of Rbohs from strawberry, grape, Arabidopsis, and rice suggested that the FvRbohs could be divided into five subgroups and showed a closer relationship with those from grape and Arabidopsis than those from rice. The expression patterns of FvRboh genes in root, stem, leaf, flower, and fruit revealed robust tissue specificity. The expression levels of FvRbohA and FvRbohD were quickly induced by cold stress, followed by an increase in NADPH oxidase activity, leading to O2− accumulation and triggering the antioxidant reaction by the transient increases in SOD activity. This suggested these two genes may be involved in cold stress and defense responses in strawberry.

Peer Review #1 of "Genomics analysis of genes encoding respiratory burst oxidase homologs (RBOHs) in jatropha and the comparison with castor bean (v0.1)"

“…In this study, a genome-wide identification and manual curation were performed, which results in seven Rboh family genes from both species. The family number is same as that present in grape and strawberry, but relatively smaller than other angiosperm plants reported thus far (Sagi & Fluhr, 2006;Cheng et al, 2013;Wang et al, 2013;Chang et al, 2016;Hu et al, 2018;Zhang et al, 2018;. The result is consistent with the fact that both jatropha and castor bean didn't experience any recent WGD, which act as a main force for expansion of genes or gene families (Qiao et al, 2019;Zou et al, 2016aZou et al, , 2017, 2019c.…”

“…Manuscript to be reviewed esculenta), ten members from arabidopsis (Arabidopsis thaliana), ten members from rubber (Hevea brasiliensis), and 15 members from wheat (Triticum aestivum) (Sagi & Fluhr, 2006;Cheng et al, 2013;Wang et al, 2013;Hu et al, 2018;Zhang et al, 2018;. Evidence showed that RBOHs play key roles in growth, development, hormone signaling, and stress responses of plants (Marino et al, 2012;Kaur et al, 2014;Angelos & Brandizzi, 2018;Wang et al, 2018;.…”

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

“…NADPH oxidases, also named respiratory burst oxidase homologues (RBOHs), require NADPH to generate the superoxide anion (O 2 - ), subsequently ending with the secondary production of other ROS such as hydrogen peroxide (H 2 O 2 ) [ 41 ]. Our previous study demonstrated that RBOHD was important in response to cold stress in strawberry [ 42 ]. As shown in Figure 6 K and Figure 7 K, the expression of FaRBOHD was markedly increased under cold treatment, and significantly higher in overexpressing FaG6PDH-CY than that in control.…”

NADPH Oxidases: The Vital Performers and Center Hubs during Plant Growth and Signaling

“…AtSRC2 is an activator of Ca 2+ -dependent AtRbohF-mediated ROS production and may play a role in cold responses [126]. Similarly, a recent study proposed that the expression levels of FvRbohA and FvRbohD in strawberry are quickly induced by cold, followed by an increase in NADPH oxidase activity [127]. Conversely, at high temperature (30°C), the total NADPH oxidase activity and NtRbohD expression are all suppressed in tobacco mosaic virus (TMV)-inoculated leaves of tobacco, which resulted in less ROS production and enhanced susceptibility to pathogen and suppression of HR-type necrosis [128,129].…”