Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Carvalho, Gardênia Braz Figueiredo de; Silva-Pereira, Rosiane Aparecida da; Pacífico, Lucila Grossi Gonçalves; Fonseca, Cristina Toscano

doi:10.1590/s0074-02762011000700009

Cited by 16 publications

(13 citation statements)

References 31 publications

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“…With this in mind, our group have recently designed an in silico strategy based in the principles of reverse vaccinology, and using a rational criteria to mine candidates in parasite genome to be used in the immunodiagnosis of schistosomiasis [72]. Six antigens were selected based on the evidence of gene expression at different phases of the parasite life cycle in the definitive host, accessibility to host immune system (exposed proteins), low similarity with human and other helminthic proteins, and presence of predicted B cells epitopes (Table 2) [72]. Although our in silico analysis led to identification of six candidates, this strategy has not been yet experimentally validated.…”

Section: Antigens To Be Used In Schistosomiasis Diagnostic Testmentioning

confidence: 99%

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Fonseca

Carvalho

Alves

et al. 2012

Journal of Parasitology Research

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The development of a vaccine against schistosomiasis and also the availability of a more sensitive diagnosis test are important tools to help chemotherapy in controlling disease transmission. Bioinformatics tools, together with the access to parasite genome, published recently, should help generate new knowledge on parasite biology and search for new vaccines or therapeutic targets and antigens to be used in the disease diagnosis. Parasite surface proteins, especially those expressed in schistosomula tegument, represent interesting targets to be used in vaccine formulations and in the diagnosis of early infections, since the tegument represents the interface between host and parasite and its molecules are responsible for essential functions to parasite survival. In this paper we will present the advances in the development of vaccines and diagnosis tests achieved with the use of the information from schistosome genome focused on parasite tegument as a source for antigens.

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Section: Antigens To Be Used In Schistosomiasis Diagnostic Testmentioning

confidence: 99%

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Fonseca

Carvalho

Alves

et al. 2012

Journal of Parasitology Research

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“…This sequence length was adopted as the minimum size to conduct the prediction of epitopes with an affinity for HLA molecules. Several studies have used exposed regions proteins from pathogens to identify antigens for the development of vaccines and diagnostic kits against several diseases …”

Section: Resultsmentioning

confidence: 99%

“…Several studies have used exposed regions proteins from pathogens to identify antigens for the development of vaccines and diagnostic kits against several diseases. [51][52][53] All 64 selected extracellular regions were used for the prediction of epitopes with affinity for HLA (DRB1 0101, DRB1 0301, DRB1 0401, DRB1 0701, DRB1 1101, and DRB1 1501). These HLA molecules were chosen because they are widely presented in different populations around the world, a desirable feature in the development of vaccines due to polymorphism in the HLA genes.…”

Section: Resultsmentioning

confidence: 99%

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

Lopes

Oliveira

Coelho

et al. 2017

Biotechnology Progress

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Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis. The three-dimensional structure of selected epitopes was constructed and used in molecular docking to verify the interaction with murine MHCII H2-IA . In this process, four epitopes were selected and synthesized to assess their ability to stimulate proliferation of CD4 T lymphocytes in mice splenocyte cultures. The results showed that Sm041370 and Sm168240 epitopes induced significant cell proliferation. Additionally, the four epitopes were used as antigens in the Indirect Enzyme-Linked Immunosorbent Assay (ELISA) to assess the recognition by serum from individuals infected with Schistosoma mansoni. Sm140560, Sm168240, and Sm041370 epitopes were recognized by infected individuals IgG antibodies. Therefore, Sm041370 and Sm168240 epitopes that stood out in in silico and in vitro analyses could be promising antigens in schistosomiasis vaccine development or diagnostic kits. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:804-814, 2017.

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“…Using bioinformatics screening, another group succeeded in identifying several novel diagnostic antigen candidates from the previously sequenced S. mansoni genome (Berriman et al, 2009). Overall, four criteria were used in the in silico analysis strategy, including expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes (Carvalho et al, 2011). As a result, six promising diagnostic antigens (Sm200, Sm12.8, Sm43.5, Sm127.9, Sm18.9, Sm16.5) were elicited from 13,197 transcripts described for S. mansoni (Berriman et al, 2009).…”

Section: The “New Generation” Diagnostic Methods For Schistosomiasis mentioning

confidence: 99%

Development of â€œ-omicsâ€ research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis

Wang

2014

Front. Microbiol.

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Schistosomiasis, caused by dioecious flatworms in the genus Schistosoma, is torturing people from many developing countries nowadays and frequently leads to severe morbidity and mortality of the patients. Praziquantel based chemotherapy and morbidity control for this disease adopted currently necessitate viable and efficient diagnostic technologies. Fortunately, those “-omics” researches, which rely on high-throughput experimental technologies to produce massive amounts of informative data, have substantially contributed to the exploitation and innovation of diagnostic tools of schistosomiasis. In its first section, this review provides a concise conclusion on the progresses pertaining to schistosomal “-omics” researches to date, followed by a comprehensive section on the diagnostic methods of schistosomiasis, especially those innovative ones based on the detection of antibodies, antigens, nucleic acids, and metabolites with a focus on those achievements inspired by “-omics” researches. Finally, suggestions about the design of future diagnostic tools of schistosomiasis are proposed, in order to better harness those data produced by “-omics” studies.

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Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Cited by 16 publications

References 31 publications

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

Development of â€œ-omicsâ€ research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis

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Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Cited by 16 publications

References 31 publications

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Schistosoma Tegument Proteins in Vaccine and Diagnosis Development: An Update

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

Development of â€œ-omicsâ€ research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis

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About

Development of â€œ-omicsâ€ research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis