Identification of schistosoma haematobium, S. bovis and S. curassoni by multivariate analysis of cercarial papillae indices

Bayssade-Dufour, Ch.; Cabaret, Jacques; Ngendahayo, L. D.; Albaret, J.-L.; Carrat, C.; Chabaud, A. G.

doi:10.1016/0020-7519(89)90109-4

Cited by 12 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Papillary patterns were reported from various trematode species, e.g., Diplostomum sp. (Niewiadomska 1996) and some species of the family Schistosomatidae (Bayssade-Dufour et al 1989). Chaetotaxy of bird schistosome cercariae was studied by several authors.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cercarial dimensions and surface structures as a tool for species determination of Trichobilharzia spp.

Podhorský¹,

Huůzová²,

Mikeš

et al. 2009

Acta Parasitologica

View full text Add to dashboard Cite

Cercariae of bird schistosomes are traditionally considered to be very similar in their morphological characteristics. In order to solve the problem, we tested some methods which might be suitable for cercarial differentiation. Fourteen isolates of three Trichobilharzia species (T. szidati, T. franki, T. regenti) occurring sympatrically in Central Europe were used. Dimensions of individual cercariae do not represent a useful criterion for identification, because the intraspecific variability exceeds the interspecific one. On the other hand, chaetotaxy appears a promising way for discrimination, although some sensory papillae do not stain sufficiently with silver nitrate. The papillary pattern (i.e. number and relative position of papillae) is specific for all Trichobilharzia species studied by us. Therefore, we compiled an identification key for the three Trichobilharzia species. In addition, we tried to find species-specific surface saccharide epitopes; none of the labeled lectin probes can be used as a speciesspecific marker.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Bayssade-Dufour et al 1989, Niewiadomska 1996. In bird schistosomes, chaetotaxy of cercariae was described for one species of the genus Austrobilharzia (Abdul-Salam and Screelatha 2004) and several species of the genus Trichobilharzia: e.g.…”

Section: Introductionmentioning

confidence: 99%

Cercarial dimensions and surface structures as a tool for species determination of Trichobilharzia spp.

Podhorský¹,

Huůzová²,

Mikeš

et al. 2009

Acta Parasitologica

View full text Add to dashboard Cite

show abstract

“…Methods exist to differentiate cercariae of the two species but they require specialized laboratory techniques. [17][18][19][20][21] The standard approach to this problem is to infect lab hamsters, which must then be maintained for twelve weeks to produce the adult worms that may be used in species-level diagnostic techniques which rely on the morphology of adult worms, 22 isoenzyme profiles, [23][24][25][26] Southern blot hybridization with labeled probes, 27 randomly amplified DNA polymorphisms, [28][29][30] or comparative morphology of resultant eggs. 31 Future epidemiological studies would be aided by the availability of an expedient, PCRbased approach that allows for specific identification of individuals from both species, whether at the cercaria or adult stage.…”

Section: Introductionmentioning

confidence: 99%

PCR-RFLP analysis of the ITS2 region to identify Schistosoma haematobium and S. bovis from Kenya.

Barber¹,

Mkoji²,

Loker³

2000

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Schistosoma haematobium, primarily a human parasite, and the closely related Schistosoma bovis from ruminants, are sympatric in many African countries such as Kenya. Because these two species 1) can inhabit the same Bulinus snails, 2) may be found in the same freshwater habitat, and 3) have morphologically similar cercariae, better means are needed to tell them apart. The second internal transcribed spacer (ITS2) region of the ribosomal gene complex (rDNA) of recent Kenyan isolates of both species was sequenced and found to be a 98% match. The S. bovis sequences were nearly identical (99%) to conspecific sequences from Niger; the S. haematobium sequences were nearly identical (99%) to conspecific sequences from Egypt, Mali, and Niger. Restriction fragment length polymorphism (RFLP) analysis of a 480 base pair (bp) PCR product containing the ITS2 region using two restriction enzymes, Taq1 and Sau3A1, yielded species-specific fragment patterns that allowed successful identification of a single S. haematobium cercaria. The protocol outlined here is useful in providing a rapid, one-day identification of S. haematobium (and likely S. bovis) cercariae (the infective larval stage) and/or other life cycle stages in a basic molecular biology laboratory. By helping to determine whether schistosome-infected bulinid snails in a particular body of water are transmitting a human or an animal schistosome, or both, this analysis will aid in disease control and in ongoing epidemiological studies.

show abstract

“…Attempts were made to estimate three CI, named AD, AL and U after the sensory receptors, for each cercaria (Bayssade-Dufour et al, 1989;Cabaret et al, 1990). Only cercariae for which full or almost full data were available were included in the final analysis.…”

Section: Methodsmentioning

confidence: 99%

“…In their studies of Schistosoma mansoni, for example, Bayssade-Dufour (1977) and Cassone et al (1981) identified variation, in both the number and position of sensory receptors, between cercariae produced from parasites collected from humans in the West Indies and those produced from parasites collected from African rodents. Bayssade-Dufour et al (1989) described a multivariate statistical procedure, based on the estimation of various cercarial indices (CI), including measurements of the position of antero-dorsal (AD), antero-lateral (AL) and tail-stem (U) sensory receptors, that could be used to distinguish three species of Schistosoma. Subsequently, Cabaret et al (1990) used a modification of the same procedure to distinguish four African species of schistosomes.…”

mentioning

confidence: 99%

Phenotypic plasticity in the cercarial indices of human and rodent samples ofSchistosoma mansoni(Digenea: Schistosomatidae)

Bogéa¹

2005

Annals of Tropical Medicine & Parasitology

View full text Add to dashboard Cite

In the epidemiology of schistosomiasis, the cercarial index (CI) has been used for several purposes, including the identification of the species of vertebrate host involved in transmission in a particular setting. The CI of samples of Schistosoma mansoni of human (BH) and rodent (CMO) origin have now been compared, in an attempt to identify morphological markers on cercariae that would indicate whether the eggs from which the cercariae have developed were produced in humans or in rodents. After silver-nitrate impregnation of the cercariae, the CI (and their reaction norms) for the antero-dorsal (AD), antero-lateral (AL) and tail-stem (U) sensory receptors were investigated. The data analysis was based on linear-regression methods, Levene's test for the equality of variances, pooled-variance independent t-tests, and discriminant analysis. The results indicated that CI varied with vertebrate-host origin, that the AD reaction norms were more consistent and plastic than the other reaction norms investigated, that there appeared to be no simple allometric relationship between any of the CI investigated and cercarial body length, and that the BH sample gave larger standard deviations and a higher incidence of mis-classification than the CMO, indicating some developmental instability in the sample of human origin. The relative instability of the BH sample was, however, possibly the result of the experimental procedures. As the parasites used to produce the BH sample had been isolated from humans fairly recently and then routinely maintained in mice, host-induced selection could have affected the sample, altering its CI and their reaction norms. The results of the discriminant analysis indicated that, of the indices investigated, AD was the best morphological marker for distinguishing human and rodent samples of S. mansoni.

show abstract

Identification of schistosoma haematobium, S. bovis and S. curassoni by multivariate analysis of cercarial papillae indices

Cited by 12 publications

References 5 publications

Cercarial dimensions and surface structures as a tool for species determination of Trichobilharzia spp.

Cercarial dimensions and surface structures as a tool for species determination of Trichobilharzia spp.

PCR-RFLP analysis of the ITS2 region to identify Schistosoma haematobium and S. bovis from Kenya.

Phenotypic plasticity in the cercarial indices of human and rodent samples ofSchistosoma mansoni(Digenea: Schistosomatidae)

Contact Info

Product

Resources

About