Identification of Scavenger Receptor SR-BI as a High Density Lipoprotein Receptor

Acton, Susan; Rigotti, Attilio; Landschulz, Katherine T.; Xu, Shangzhe; Hobbs, Helen H.; Krieger, Monty

doi:10.1126/science.271.5248.518

Cited by 2,155 publications

(1,965 citation statements)

References 38 publications

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“…SR-BI is highly-expressed in the liver and plays a fundamental role in reverse cholesterol transport mediating selective uptake of cholesteryl esters and promoting biliary cholesterol secretion [34–36]. Loss-of-function variants in human SCARB1 (SR-BI) were associated with increased in circulating HDL-C levels [37].…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Allen

Zhao

Solano

et al. 2018

J of Extracellular Vesicle

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To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), we developed a novel pipeline to overcome current limitations in analysis entitled, “Tools for Integrative Genome analysis of Extracellular sRNAs (TIGER)”. To demonstrate the power of this tool, sRNA-seq was performed on mouse lipoproteins, bile, urine and livers. A key advance for the TIGER pipeline is the ability to analyse both host and non-host sRNAs at genomic, parent RNA and individual fragment levels. TIGER was able to identify approximately 60% of sRNAs on lipoproteins and >85% of sRNAs in liver, bile and urine, a significant advance compared to existing software. Moreover, TIGER facilitated the comparison of lipoprotein sRNA signatures to disparate sample types at each level using hierarchical clustering, correlations, beta-dispersions, principal coordinate analysis and permutational multivariate analysis of variance. TIGER analysis was also used to quantify distinct features of exRNAs, including 5ʹ miRNA variants, 3ʹ miRNA non-templated additions and parent RNA positional coverage. Results suggest that the majority of sRNAs on lipoproteins are non-host sRNAs derived from bacterial sources in the microbiome and environment, specifically rRNA-derived sRNAs from Proteobacteria. Collectively, TIGER facilitated novel discoveries of lipoprotein and biofluid sRNAs and has tremendous applicability for the field of extracellular RNA.

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Section: Resultsmentioning

confidence: 99%

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Allen

Zhao

Solano

et al. 2018

J of Extracellular Vesicle

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“…Even though worm homologues of these receptors are predicted, their function and localization is unknown. A BLASTP 19 search of the murine scavenger receptor-B (SR-B) 20,21 sequence against the C. elegans genome yielded six candidate genes ( Supplementary Tables S1 -S3 ). Whether these SR-B candidate receptors are indeed present on coelomocytes and responsible for I-switch uptake was studied by knocking down these proteins using RNA interference (RNAi) 22 .…”

Section: Resultsmentioning

confidence: 99%

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

et al. 2011

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Structural DNA nanotechnology seeks to build synthetic molecular machinery from DNA. DNA nanomachines are artifi cially designed assemblies that switch between defi ned conformations in response to an external cue. Though it has proved possible to create DNA machines and rudimentary walkers, the function of such autonomous DNA-based molecular devices has not yet been achieved inside living organisms. Here we demonstrate the operation of a pH-triggered DNA nanomachine inside the nematode Caenorhabditis elegans . The nanomachine uses fl uorescence resonance energy transfer to effectively map spatiotemporal pH changes associated with endocytosis in wild type as well as mutant worms, demonstrating autonomous function within the organismal milieu in a variety of genetic backgrounds. From this fi rst demonstration of the independent functionality of a DNA nanomachine in vivo , we observe that rationally designed DNA-based molecular devices retain their in vitro functionality with quantitative precision. This positions DNA nanodevices as exciting and powerful tools to interrogate complex biological phenomena.

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“…In CaCo-2 cells, differential and competitive uptake of α-carotene, β-carotene, lutein, and LYC has been reported [34]. Recently, scavenger receptor class B, type I (SR-BI) has been identified as a protein involved in intestinal transport of carotenoids in Caco-2 cells [35], and SR-BI is highly concentrated in human and rat liver and in steroidogenic tissues [36,37]. The specificity of transport and/or binding proteins is unclear, yet it is plausible that this specificity may depend on the number and/or location of conjugated double bonds present within carotenoids ( Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Phytoene, phytofluene, and lycopene from tomato powder differentially accumulate in tissues of male Fisher 344 rats

Campbell¹,

Engelmann²,

Lila³

et al. 2007

Nutrition Research

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Tomato product consumption is inversely related to prostate cancer incidence, and lycopene (LYC) has been implicated in reduced prostate cancer risk. The contribution of other tomato carotenoids, phytoene (PE) and phytofluene (PF), towards prostate cancer risk has not been adequately studied. The relative uptake and tissue distribution of tomato carotenoids are not known. We hypothesize that PE and PF are bioavailable from a tomato powder diet or from a purified source and accumulate in androgen-sensitive tissues. In this study, 4 wk old male Fisher 344 rats were pre-fed an AIN-93G powder diet composed of 10% tomato powder containing PE, PF, and LYC (0.015, 0.012, and 0.011 g/kg diet, respectively). After 30 d tomato powder feeding, hepatic PF concentrations (168 ± 20 nmol/ g) were higher than PE or LYC (104 ± 13 and 104 ± 13 nmol/g, respectively). In contrast, LYC, followed by PF, had the highest accumulation of the measured carotenoids in the prostate lobes and seminal vesicles. When tomato powder-fed rats received a single oral dose of either ∼2.7 mg PE or PF, an increase in the dosed carotenoid concentration was observed in all measured tissues, except the adrenal. Percent increases of PF were greater than that of PE in liver, serum, and adipose (37, 287 and 49% versus 16, 179 and 23%, respectively). Results indicate that the relative tomato carotenoid biodistribution differs in liver and androgen-sensitive tissues, suggesting that minor changes in the number of sequential double bonds in carotenoid structures alter absorption and/or metabolism of tomato carotenoids.

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Identification of Scavenger Receptor SR-BI as a High Density Lipoprotein Receptor

Cited by 2,155 publications

References 38 publications

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

Phytoene, phytofluene, and lycopene from tomato powder differentially accumulate in tissues of male Fisher 344 rats

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