Identification of salmonid fish using microsatellite markers with identical PCR-primers

The evolution of microsatellite loci in salmonid fishes

“…As was mentioned above, the examined loci could be used for the identification of different salmonid species because of polymorphic differences and allele sizes [14]. The table presents the data on the polymor phism of the examined microsatellite loci in different species of salmonid fishes.…”

“…Using these homologous primers, the effectiveness of microsatel lite loci for the identification of salmonid fishes was demonstrated [14]. Using these primers for allele sequencing, the nucleotide sequences of the One103 [15], One109 [15], OMM1037 [16], and Ots102 [17] were determined in all of the analyzed species.…”

“…The One103 locus was first identified in sockeye salmon [14]. The locus has a complex, interrupted structure and includes two types of tetranucleotide repeats, (TCTA) n and (TCAT) n .…”

Hybrids between chum Oncorhynchus keta and pink Oncorhynchus gorbuscha salmon: age, growth and morphology and effects on salmon production

“…DNA isolation, PCR amplification and genotyping procedures are described in Zhivotovsky et al . (). Identification of the sex of the parents of the hybrids was accomplished with diagnostic length polymorphisms in two regions of the mitochondrial DNA, ND3–ND4 and COI–COII/A8 , which were amplified with specific primers (Gharrett et al ., ) and cut with restriction enzymes Dde I, Msp I and Rsa I.…”

“…Fin clips from the hybrids and parental species were fixed in 96% ethanol. In order to confirm the putative hybrids, PCR primers were used to amplify microsatellite markers OMM‐1050, which is diagnostic for the respective species (Zhivotovsky et al ., ) and OMM‐1015, which has different allele size ranges for O. keta and O. gorbuscha . DNA isolation, PCR amplification and genotyping procedures are described in Zhivotovsky et al .…”