Identification of Salmonellae of Serogroup C1 by Immunofluorescence and Co-agglutination with Antiserum Against an Oligosaccharide-protein Conjugate

Ekwall, Erik; Svenson, S. B.; Lindberg, Alf A.

doi:10.1099/00222615-15-2-173

Cited by 14 publications

(14 citation statements)

References 12 publications

(9 reference statements)

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“…This inconsistency may be explained as follows: Manβ1-2Manα1-HPO 3 -of C. glabrata mannan with relatively short α-linked side-chains is located in a favorable position readily recognized by serum no. 6 on the cell surface, whereas Recently, the antigenic cross-reactivity between Candida species and some Salmonella species has been observed (Ekwall et al 1982;Nnalue et al 1991). The Salmonella C 1 O-polysaccharide has a considerable amount of β-1,2-linked mannose residue, which was also observed in Candida mannans (Shibata et al 1985;Lindberg et al 1988).…”

Section: Discussionmentioning

confidence: 93%

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

Kobayashi

Oyamada

Iwadate

et al. 1998

Archives of Microbiology

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A mannan of Candida glabrata IFO 0622 digested by Arthrobacter exo-alpha-mannosidase and a beta-1,2-linked mannobiose obtained from the parent mannan by acid treatment was analyzed using 13C nuclear magnetic resonance spectroscopy. The results show that the beta-1, 2-linked mannobiosyl residue is esterified to a phosphate group through position C-1 in the alpha-configuration, Manbeta1- 2Manalpha1-HPO3-. The results of immunochemical assays of these mannans using the commercial antigenic factor sera of the genus Candida (Candida Check, Iatron) indicate that the main recognition site of serum no. 6 in this kit is the mannotetraosyl side-chain Manbeta1-2Manalpha1- 2Manalpha1-2Man in C. glabrata mannan and also suggest that the phosphate-containing unit (such as Manbeta1- 2Manalpha1-HPO3- in this mannan) behaves as one of the antigenic determinants of serum no. 6, but not of serum no. 5. Therefore, the present and previous findings indicate that serum no. 5 recognizes relatively longer beta-1,2-linked oligomannosyl side-chains, Manbeta1-[2Manbeta1-]n 2Man (n = 1-6), attached to the phosphate groups previously observed in the cell wall mannans of Candida albicans, Candida stellatoidea, and Candida tropicalis.

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Section: Discussionmentioning

confidence: 93%

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

Kobayashi

Oyamada

Iwadate

et al. 1998

Archives of Microbiology

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“…More recently Lindberg et al (1988) reported that the 0 : 6,,6,,7 PS of S. thompson strain IS40 comprises two populations of chains which differ only because one population lacks the a-D-glUCOpyranOSyl group 3-linked to the a-D-mannopyranosyl 111 residue of each repeat unit. As the non-glucosylated, phage-14-endomannosidasesusceptible population has been shown to display only the 0 : 7 specificity (Ekwall et al, 1982), it follows that chains substituted with D-glucose at the a-Dmannopyranosyl I11 residue have both the 0 : 6 , and 0 : 6, determinants. Taken together, these previous studies would tend to suggest that the same structural element is responsible for both the 0 : 6 , and 0 : 6 , determinants.…”

Section: Discussionmentioning

confidence: 99%

“…The other mAbs did not react with mannan or with LPS from other serogroups. They were titrated against another set of antigens comprising LPS from Salmonella strains of well characterized O-antigenic specificities and a glycoconjugate specific for 0 factor 7 (Ekwall et al, 1982) ; this glycoconjugate is hereinafter referred to as CO-BSA. The results ( Table 1, Fig.…”

Section: Generation Of Mabs and Characterization Of Their Antigenic Smentioning

confidence: 99%

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Nnalue

Lindberg

1997

Microbiology

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2 Karol i nska I nst it Ute,The 0-antigenic specificities found among salmonellae of serogroup C, are 0:6,,7,0:6,,7,0:6,,6,, 7 and 0:6,7,14, as defined by classical serology. Factor 0:7 is the group-wide determinant while factors O:6,, 0:6, and 0:14 are found in some strains but not others. Strains of the 0:6,,7 specificity are subject to lysogenic conversion by phages 61 and 14 to the 0:6,,7 and 0:6,7,14 specificities, respectively. To further delineate antigenic complexity and serological relationships among strains of this serogroup monoclonal antibodies (mAbs) were generated against the 0: 6,,6,,7 polysaccharide of Salmonella thompson. Five mAbs of either the O:6, or the O:6, specificities did not bind 0:6,7,14 strains or LPS, showing that the 0:6 determinant in these strains is neither O:6, nor O:6,. Thus antigenic conversion of 0:6,,7 strains by phage 14 is accompanied by addition of 0:14 as well as loss of O:6,. Three mAbs which demonstrated group-wide reactivity, and were thus specific for 0: 7, recognized clearly separable epitopes hereby defined as sub-specificities, 0: 7,, 0: 7, and 0:7,. lmmunoblotting of mAbs against electrophoretically resolved LPS showed that factors O:6, and 0:6, are expressed only in LPS molecules of high molecular mass whereas 0:7, and 0:7, are expressed only in relatively low-molecular-mass chains. These results are consistent with the expression of different antigenic determinants in structurally distinct subpopulations of 0 chains. The implication of the existence of distinct subpopulation of chains is that the published structure of the 0:6,7 repeat unit is not fully representative of the 0-antigenic structure of this group.

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“…The glycoconjugates specific for serogroups A, B or D consist of synthetic disaccharides covalently coupled to BSA as described previously (Kallin et al, 1986). The serogroups E-specific and C,-specific glycoconjugates consist respectively of a synthetic trisaccharide and a decasaccharide derived by phage hydrolysis of lipopolysaccharide (Ekwall et al, 1982) and also coupled to BSA as above.…”

Section: Mannan Lipopolysaccharides and Glycoconjugatesmentioning

confidence: 99%

“…A cross-reactivity between the mannan of Candida species and the 0 antigen i.e. polysaccharide of Salmonella serogroup C, using a rabbit polyclonal antiserum elicited with a synthetic trisaccharide-BSA conjugate has been described (Ekwall et al, 1982). We have confirmed this finding when we isolated a rat monoclonal antibody, MASCl-MR9 (MR9), which not only reacted with both these antigens but also with the 0 antigen of Sulmonella species serogroup E (Nnalue et al, 1991 j. The mannans of Candida species are highly complex branched molecules comprising mainly mannose in various linkages.…”

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confidence: 99%

Cross‐reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C₁ and E O‐antigens is mediated by a terminal non‐reducing β‐mannosyl residue

Nnalue

Weintraub

Oscarson

et al. 1994

European Journal of Biochemistry

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Rat monoclonal antibody MASCl-MR9 (MR9) binds to a mannan of Candida species and the 0-antigenic polysaccharides of Salmonella bacteria of serogroups C, (CO) and E (EO). Saccharides (3 -10 residues) of mammalian origin with terminal and internal Manpa-1-2, Manpa-1+3 and Manpa-1-6 residues also failed to inhibit at any concentration. None of the saccharides with internal P-mannosyl residue was able to inhibit the MR9 antibody. Monosaccharides D-mannose, P-D-Manp-1 -0Me and 1 3 anhydro-D-mannitol inhibited the MR9 monoclonal antibody whereas a-D-Manp-1 -OMe, P-D-Glcp-1 -OMe, and 8-D-Galp-1-OMe did not. In addition a Klebsiella K24 capsular polysaccharide containing a P-~-Manp(l+4)-a-~-GIcA (GlcA, glucuronic acid) as a structural element possessed an inhibitory activity. MR9 therefore recognizes an epitope within p-mannose monosaccharide residues at the terminal non-reducing ends of carbohydrate chains in mannan, and polysaccharides in Salmonella serogroups CO and EO and Klebsiella K24.Protein carbohydrate interactions are involved in many biological processes such as the binding of antibodies to their epitopes and attachment of bacteria, viruses and hormones to their receptors (Lentz, 1990;Quiocho, 1986). The molecular mechanisms for recognition in these interactions in many instances still remain to be fully delineated. Immunochemical specificity is one aspect of molecular recognition which currently commands high interest. The composition of epitopes in biological molecules and the factors that govern their recognition by antibody are complex and poorly understood. This complexity is especially true for carbohydrates where sequence as well as linkage are involved in the determination of immunochemical specificity.Our laboratory has been involved in the use of monoclonal antibodies for definition of structure/function relationships and identification of the structural bases for molecular recognition , Luk et al., 1991, Nnalue et al., 1992 Abbreviations. I,,,, the concentration of an inhibitor necessary to lower the antibody titer with 50% relative to controls with no inhibitor added; Kdo, 3-deoxy-~-manno-octu~osonic acid; Rha, rhamnose; GlcA, glucuronic acid. microbial antigens are well-suited for the investigation of epitopes. It is at present not clear whether all epitopes recognized by a single molecular species of antibody are identical or, at least, similar and whether all antigens which crossreact share identical epitopes. A cross-reactivity between the mannan of Candida species and the 0 antigen i.e. polysaccharide of Salmonella serogroup C, using a rabbit polyclonal antiserum elicited with a synthetic trisaccharide-BSA conjugate has been described (Ekwall et al., 1982). We have confirmed this finding when we isolated a rat monoclonal antibody, MASCl-MR9 (MR9), which not only reacted with both these antigens but also with the 0 antigen of Sulmonella species serogroup E (Nnalue et al., 1991 j. The mannans of Candida species are highly complex branched molecules comprising mainly mannose in various linkages....

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Identification of Salmonellae of Serogroup C1 by Immunofluorescence and Co-agglutination with Antiserum Against an Oligosaccharide-protein Conjugate

Cited by 14 publications

References 12 publications

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Cross‐reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C₁ and E O‐antigens is mediated by a terminal non‐reducing β‐mannosyl residue

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Identification of Salmonellae of Serogroup C1 by Immunofluorescence and Co-agglutination with Antiserum Against an Oligosaccharide-protein Conjugate

Cited by 14 publications

References 12 publications

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata

O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains

Cross‐reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C1 and E O‐antigens is mediated by a terminal non‐reducing β‐mannosyl residue

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Cross‐reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C₁ and E O‐antigens is mediated by a terminal non‐reducing β‐mannosyl residue