Identification of salivary proteins in the whitefly <i>Bemisia tabaci</i> by transcriptomic and LC–MS/MS analyses

Huang, Hai‐Jian; Ye, Zhuang-Xin; Lu, Gang; Zhang, Chuan-Xi; Chen, Jianping; Li, Junmin

doi:10.1111/1744-7917.12856

Cited by 51 publications

(68 citation statements)

References 62 publications

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“…The gland lumens empty into primary or accessory salivary gland ducts, which are connected to each other (Ghanim et al, 2001). The primary salivary glands of all hemipterans investigated thus far contain multiple cell types that each have different kinds of electron-dense secretory vesicles (Sogawa, 1968;Wayadande et al, 1997;Ghanim et al, 2001;Reis et al, 2003;Ammar et al, 2017;Dai et al, 2019), and produce and secrete salivary components such as proteins (Sogawa, 1968;Mutti et al, 2008;Yang et al, 2017;Su et al, 2019;Xu et al, 2019;Huang et al, 2020), long non-coding RNAs (Chen et al, 2020), and small RNAs (Van Kleeff et al, 2016). Some of these secreted salivary component are effectors or elicitors, but some have other functions, such as structural roles in salivary sheaths (Cohen et al, 1998;Freeman et al, 2001;Will and Vilcinskas, 2015), and others may play a role in both (Shangguan et al, 2018).…”

Section: The Identification Of Effectorsmentioning

confidence: 99%

“…The salivary transcriptome varies with different diets or plant species (Jonckheere et al, 2016;Rivera-Vega et al, 2018: Huang et al, 2020, the presence of viruses (He et al, 2020) or endosymbionts (Wang et al, 2020). For example, TYLCV alters gene expression in B. tabaci salivary glands where it replicates and this also occurs in the presence of the non-replicating papaya leaf curl China virus (PaLCuCNV) (He et al, 2020).…”

Section: The Identification Of Effectorsmentioning

confidence: 99%

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Spotlight on the Roles of Whitefly Effectors in Insect–Plant Interactions

et al. 2021

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The Bemisia tabaci species complex (whitefly) causes enormous agricultural losses. These phloem-feeding insects induce feeding damage and transmit a wide range of dangerous plant viruses. Whiteflies colonize a broad range of plant species that appear to be poorly defended against these insects. Substantial research has begun to unravel how phloem feeders modulate plant processes, such as defense pathways, and the central roles of effector proteins, which are deposited into the plant along with the saliva during feeding. Here, we review the current literature on whitefly effectors in light of what is known about the effectors of phloem-feeding insects in general. Further analysis of these effectors may improve our understanding of how these insects establish compatible interactions with plants, whereas the subsequent identification of plant defense processes could lead to improved crop resistance to insects. We focus on the core concepts that define the effectors of phloem-feeding insects, such as the criteria used to identify candidate effectors in sequence-mining pipelines and screens used to analyze the potential roles of these effectors and their targets in planta. We discuss aspects of whitefly effector research that require further exploration, including where effectors localize when injected into plant tissues, whether the effectors target plant processes beyond defense pathways, and the properties of effectors in other insect excretions such as honeydew. Finally, we provide an overview of open issues and how they might be addressed.

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Section: The Identification Of Effectorsmentioning

confidence: 99%

Section: The Identification Of Effectorsmentioning

confidence: 99%

Spotlight on the Roles of Whitefly Effectors in Insect–Plant Interactions

et al. 2021

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“…The midgut and saliva play critical roles in mediating the interaction between herbivorous insects and their host plants (Kumar et al, 2015;Huang et al, 2020). Some proteases are highly expressed in the midgut and may reflect the ability of insects to select their appropriate host plants for development (Saikia et al, 2011).…”

Section: The Differentially Expressed Genes Of Saliva and The Midgut Further Validate The Modules Related To Host Plant Selectionmentioning

confidence: 99%

“…Herbivorous insects can use these cysteine proteases to break down dietary proteins, and these cysteine proteases can act as defense proteins against toxins or protease inhibitors that may be produced by host plants (Koo et al, 2008;Serbielle et al, 2009). These enzymes are very important in host selection (Huang et al, 2020;Guo et al, 2020).…”

Section: The Differentially Expressed Genes Of Saliva and The Midgut Further Validate The Modules Related To Host Plant Selectionmentioning

confidence: 99%

Whitefly Network Analysis Reveals Gene Modules Involved in Host Plant Selection, Development and Evolution

Tian

Zhan²,

Dewer

et al. 2021

Front. Physiol.

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Whiteflies are Hemipterans that typically feed on the undersides of plant leaves. They cause severe damage by direct feeding as well as transmitting plant viruses to a wide range of plants. However, it remains largely unknown which genes play a key role in development and host selection. In this study, weighted gene co-expression network analysis was applied to construct gene co-expression networks in whitefly. Nineteen gene co-expression modules were detected from 15560 expressed genes of whitefly. Combined with the transcriptome data of salivary glands and midgut, we identified three gene co-expression modules related to host plant selection. These three modules contain genes related to host-plant recognition, such as detoxification genes, chemosensory genes and some salivary gland-associated genes. Results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses elucidated the following pathways involved in these modules: lysosome, metabolic and detoxification pathways. The modules related to the development contain two co-expression modules; moreover, the genes were annotated to the development of chitin-based cuticle. This analysis provides a basis for future functional analysis of genes involved in host-plant recognition.

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“…Pinfish was run with default parameters, and finally yielded polished consensus reads. Second generation Illumina sequencing was performed as we previously described (Huang et al 2020). Insect samples from 6 different tissues and 37 different development stages were analyzed.…”

Section: Transcriptomic Sequencingmentioning

confidence: 99%

Chromosome-level genome assembly of the bean bug Riptortus pedestris

Huang

et al. 2020

Preprint

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The bean bug (Riptortus pedestris) causes great economic losses of soybeans by piercing and sucking pods and seeds. Although R. pedestris has become the focus of numerous studies associated with insect–microbe interactions, plant–insect interactions, and pesticide resistance, a lack of genomic resources has limited deeper insights. In this study, we report the first R. pedestris genome at the chromosomal level using PacBio, Illumina, and Hi-C technologies. The assembled genome was 1.193 Gb in size with a contig N50 of 13.97 Mb. More than 95.7% of the total genome bases were successfully anchored to 6 unique chromosomes, with the scaffold N50 reaching 181.34 Mb. Genome resequencing of male and female individuals and chromosomic staining demonstrated that the sex chromosome system of R. pedestris is XO, and the shortest chromosome is the X chromosome. In total, 21,562 protein-coding genes were predicted, 21,320 of which were validated as being expressed in different tissues or different developmental stages. Evolutionary analysis demonstrated that R. pedestris and Oncopeltus fasciatus formed a sister group and split ∼35 million years ago. Additionally, a 5.04 Mb complete genome of symbiotic Serratia marcescens Rip1 was assembled, and the virulence factors that account for successful colonization in the host midgut were identified. The high-quality R. pedestris genome provides a valuable resource for further research, as well as for the pest management of bug pests.

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Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC–MS/MS analyses

Cited by 51 publications

References 62 publications

Spotlight on the Roles of Whitefly Effectors in Insect–Plant Interactions

Spotlight on the Roles of Whitefly Effectors in Insect–Plant Interactions

Whitefly Network Analysis Reveals Gene Modules Involved in Host Plant Selection, Development and Evolution

Chromosome-level genome assembly of the bean bug Riptortus pedestris

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