Identification of S, F1C and three PapG fimbrial adhesins in uropathogenic Escherichia coli by polymerase chain reaction

Abstract

S and F1C fimbrial adhesins often expressed by uropathogenic Escherichia coli are genetically homologous. A multiply primed polymerase chain reaction (PCR) was developed for discriminating the S (sfa) and F1C (foc) fimbrial operons. A total of 270 uropathogenic E coli strains and 80 fecal isolates were examined. PCR specifically detected the sfa and foc alleles in 105 (93%) of 113 sfa/foc+ strains by DNA hybridization. Furthermore, 87% of sfa+ uropathogenic E. coli simultaneously possessed the genes encoding the class III P fimbrial adhesin (prsG(J96)), alpha-hemolysin and cytotoxic necrotizing factor 1. Statistical analysis showed the class II P fimbrial adhesin (papG(IA2)) and F1C fimbria to be associated with high relative virulence in pyelonephritis and cystitis, respectively. The multiply primed PCR developed should be useful for assessing the contribution of the S and F1C fimbriae in the pathogenesis of urinary tract infections.

Studies on Clinical Characteristics, Urovirulence Factor and Host Susceptibility Gene in Pediatric Acute Lobar Nephronia

“…In contrast, other fimbrial and nonfimbrial adhesins (i.e. sfa, foc, and afa genes) were rarely detected among our isolates and their pathogenic roles in ALN and APN are likely of less importance, a finding similar to those reported previously in the less severe renal infection categories (Siitonen et al 1993;Blanco et al 1997;Mitsumori et al 1998). Host compromise can decrease the requirements for bacterial virulence in causing severe urinary tract infections, and, henceforth, change the distribution of papG pathogenetic determinants among the clinical isolates studied (Jantunen et al 2000;Tseng et al 2001Tseng et al , 2002Johnson and Russo 2005).…”

Coordinate Expression of Fimbriae in Uropathogenic Escherichia coli

“…However, comparison of the amino acid sequence reveals that F1C fimbriae are more closely related to S fimbriae (50). F1C fimbriae are expressed by 14 to 38% of all uropathogenic strains of E. coli (24,32,44,49). In addition, the kidney has been reported as the target tissue of F1C-expressing E. coli by using in vitro models and strictly biochemical approaches (23,51).…”

“…A total of 50 l of this bacterial suspension containing 1.55 ϫ 10 9 CFU was delivered to each mouse. At each time point (4,24,48,72, and 168 h postinfection), urine samples were collected prior to the sacrifice of eight mice. The bladder and kidneys were removed, weighed, and homogenized in PBS.…”

Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

“…These VFs can be grouped by functional category; for example, adhesins (Marklund et al, 1992;Nowicki et al, 1990) (Connell et al, 1996;Mitsumori et al, 1998), siderophore systems (Opal et al, 1990;Russo et al, 2001Russo et al, , 2002Schubert et al, 1998;Torres et al, 2001), toxins (Guyer et al, 2000;Rippere-Lampe et al, 2001b;Scott and Kaper, 1994;Welch et al, 1981), surface polysaccharides (Russo et al, 1996a;Burns and Hull, 1999), invasins (Huang et al, 1999(Huang et al, , 2001, serum resistance-associated traits (Kanukollu et al, 1985;Wooley et al, 1994), and traits of miscellaneous or unknown function (Johnson, 2003;Kurazono et al, 2000) (Table 1, Fig. 5).…”