Identification of Ruscus steroidal saponins by HPLC-MS analysis

Analysis and isolation of saponins from Limoniastrum feei by LC-UV

“…The separation of saponins using Si-gel phase liquid chromatography and gradient elution with CHCl 3 : MeOH (65:35) as mobile phase has permitted good separation of all the peaks. The conditions of detection proposed for the separation are between 15 and 30 s. TLC analysis (CHCl 3 :MeOH, 65:35) revealed four constituent in the butanol fraction (yield 0.60%, R f 0.40, 0.48, 0.53, 0.58), six constituents in the ethyl acetate extract (yield 0.42%, R f 0.48, 0.58, 0.63, 0.66, 0.78, 0.86), and five constituents in the methylene chloride (DCM) extract (yield 0.31%, R f 0.6, 0.66, 0.76, 0.80, 0.88).Liquid chromatography coupled with UV has been used in phytochemical screening and analysis of the isolated constituent from extracts [10,11].The results obtained from the LC-UV system, retention times, yields, peak areas, and tailing factors, were analyzed The obtained values confirm that this method is suitable for routine analysis [8,9].The LC-UV analysis for butanolic extract gave eight constituents. Two fractions, 6 and 7, were isolated and purified by flash chromatography (Si-gel column), eluting with H 2 O /MeCN 75:35 and affording 65 mg of 6 and 90 mg of 7.…”

“…The results obtained from the LC-UV system, retention times, yields, peak areas, and tailing factors, were analyzed The obtained values confirm that this method is suitable for routine analysis [8,9].…”

“…The other uses of Limoniastrum feei are as an antibacterial for the treatment of bronchitis and for stomach infections [4,5]. LC separation followed by derivatization and detection by fluorometric methods, as described by Waridel et al [6,7], allows a high specificity of detection.The elaboration of a more rapid detection method by LC-UV may be considered for a continuation of this work [8,9]. The liquid chromatography-UV method has been developed for the analysis and separation of saponin extracts from stems of Limoniastrum feei.…”

“…The elaboration of a more rapid detection method by LC-UV may be considered for a continuation of this work [8,9]. The liquid chromatography-UV method has been developed for the analysis and separation of saponin extracts from stems of Limoniastrum feei.…”

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

“…Ruscoside can hydrolyze into its derivatives with two alternative methods: a chemical approach based on acid hydrolysis and biotechnological approach comprise enzyme application. De Combarieu et al [26], hydrolyzed compound of R. colchicus (20 mg) with 10 mg of β-glucosidase and 5 mL of phosphoric acid (0.5%) at 37°C in 24 h, which yielded spirostan sapogenin. Contrast to this approach, enzyme hydrolysis has mild reaction conditions owing to this, it is more selective and a green process [29,30].…”