Identification of rice seed infection routes of Fusarium fujikuroi inciting bakanae disease of rice

Sunani, Sunil Kumar; Bashyal, Bishnu Maya; Kharayat, Bhupendra Singh; Prakash, Ganesan; Krishnan, S. Gopala; Aggarwal, Rashmi

doi:10.1007/s42161-019-00390-8

Cited by 25 publications

(20 citation statements)

References 30 publications

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“…F. fujikuroi is known to dominate in rice kernels and not many other crops (Sunani et al 2019). Confirming previous findings, those dominant species were capable of producing, depending on the isolate, relatively high amount of MON and BEA (Desjardins et al 1997;Leslie and Summerell 2006), but F. fujikuroi was capable of producing all four classes.…”

Section: Discussionsupporting

confidence: 83%

Fusarium fujikuroi species complex in Brazilian rice: Unveiling increased phylogenetic diversity and toxigenic potential

Nicolli

Haidukowski

Susca

et al. 2020

International Journal of Food Microbiology

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Fusarium fujikuroi species complex (FFSC) species are commonly encountered infecting rice, but knowledge of the diversity and toxigenic potential of the species is lacking in Brazil, the largest rice-producing country outside Asia. One hundred FFSC isolates obtained from national rice were identified using morphology and phylogeny of TEF, CAL and TUB genes. Eight previously known and one new phylogenetic species were identified. Three species accounted for around 60% of the strains: F. fujikuroi (n = 23), F. proliferatum (n = 22) and F. verticillioides (n = 16). The less frequent species were F. volatile (n = 8), F. anthophilum (n = 6), F. pseudocircinatum (n = 4), F. sterilihyphosum (n = 2) and F. begoniae (n = 1). The new Fusarium sp. was represented by 18 isolates. All species produced at least one of the analyzed mycotoxins [beauvericin (BEA), fumonisins (FBs), moniliformin (MON) and enniatins (ENNs)]. BEA was produced by all species but F. verticillioides. The FBs (mainly FB1) were produced mostly by F. fujikuroi, F. proliferatum and F. verticillioides. F. begoniae and F. verticillioides did not produce ENNs and F. sterilihyphosum and F. begoniae did no produce MON, while the other species produced MON and ENNs. Our results contribute new knowledge of the diversity, geographical distribution and hosts of FFSC species.

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Section: Discussionsupporting

confidence: 83%

Fusarium fujikuroi species complex in Brazilian rice: Unveiling increased phylogenetic diversity and toxigenic potential

Nicolli

Haidukowski

Susca

et al. 2020

International Journal of Food Microbiology

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“…The relationships among F. concentricum, F. fujikuroi, F. proliferatum and both isolates are unclear, but they share the same ITS sequence with a specimen of the first mentioned species, while only one base change differentiates their ITS sequence from those obtained from the type material of F. fujikuroi and several specimens of F. proliferatum. Some members of the FFSC cause severe diseases in economically important plants [82][83][84][85][86]. Moreover, F. fujikuroi is known to act as an entomopathogenic fungi but, to our knowledge, only F. proliferatum has been previously associated to mycotic infections in turtle eggs [87].…”

Section: Discussionmentioning

confidence: 99%

Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

García-Martín

Sarmiento-Ramírez

Diéguez-Uribeondo

2021

JoF

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The endangered yellow-spotted river turtle (Podocnemis unifilis) has experienced a dramatic population decline in the Ecuadorian Amazonia, mainly due to overexploitation of its eggs. To reverse this trend, the Wildlife Conservation Society has developed a head-start program in Yasuní National Park since 2008, but the potential risk that microbes associated with its eggs might represent for hatching success has not been evaluated yet. Members of the Fusarium solani species complex (FSSC) are involved in egg failure in sea turtles under natural and hatchery conditions, but their role in infecting the eggs of P. unifilis is unknown. In this study, we collected eggshells of P. unifilis and obtained 50 fungal and bacterial isolates. Some potentially pathogenic fungi of the genera Fusarium, Penicillium and Rhizopus were identified based on molecular data. Most importantly, the sea turtle pathogenic species F. keratoplasticum not only was present, but it was the most frequently found. Conversely, we have also isolated other microorganisms, such as Pseudomonas or Phoma-like species, producing a wide spectrum of antifungal compounds that may have a protective role against fungal diseases. Our survey provides useful information on potential pathogens found in P. unifilis eggshells, upon which the success of conservation programs may depend.

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“…The study highlighted that the microbiological component present on the seed surface did not affect the incidence of the disease, while the endophytic component of F. fujikuroi was relevant. Sunani et al [41] hypothesized that the fungus can infect seeds and reach the embryo in three ways: by systemic infection of the vascular system, by stylar canal, or through the growth of hyphae from the husk to the ovary. Furthermore, the microconidia of F. verticillioides can represent a source of infection of the embryo [42] and it is possible that the conidia of F. fujikuroi also follow this inoculation mechanism and systemically infect the seeds [41].…”

Section: Discussionmentioning

confidence: 99%

Optimization of a Loop-Mediated Isothermal Amplification Assay for On-Site Detection of Fusarium fujikuroi in Rice Seed

et al. 2021

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Fusarium fujikuroi, causing bakanae disease, is one of the most important seedborne pathogens of rice, the detection of which is paramount for seed certification and for preventing field infections. Molecular tests—qPCR and loop-mediated isothermal amplification (LAMP)—are replacing the blotter test in seed health procedures, due to higher sensitivity, specificity, fast turnaround results delivery, on-site application and the possibility of quantifying endophytic seed infections. A LAMP test, which had been previously developed with primers designed to target the elongation factor 1-α sequence of F. fujikuroi, was validated according to the international validation standard (EPPO, PM7/98) on thirty-four rice seed lines of different levels of susceptibility to the disease, thus comparing it to the blotter test and with two different DNA extraction procedures. The use of crude extracted DNA provided more sensitive results than the DNA extracted with the commercial kit Omega E.Z.N.A® Plant DNA kit. The results showed that the endophytic infection of F. fujikuroi is essential for the development of the disease in the field and that the minimum amount of the pathogen necessary for the development of the disease corresponds to 4.17 × 104 cells/µL. This study confirms the applicability of the LAMP technique on-site on rice seeds with fast and quantitative detection of the pathogen.

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Identification of rice seed infection routes of Fusarium fujikuroi inciting bakanae disease of rice

Cited by 25 publications

References 30 publications

Fusarium fujikuroi species complex in Brazilian rice: Unveiling increased phylogenetic diversity and toxigenic potential

Fusarium fujikuroi species complex in Brazilian rice: Unveiling increased phylogenetic diversity and toxigenic potential

Beyond Sea Turtles: Fusarium keratoplasticum in Eggshells of Podocnemis unifilis, a Threatened Amazonian Freshwater Turtle

Optimization of a Loop-Mediated Isothermal Amplification Assay for On-Site Detection of Fusarium fujikuroi in Rice Seed

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