Identification of rice blast resistance genes using international monogenic differentials

Wang, J.C.; Jia, Yulin; Wen, Jianqiang; Liu, W.P.; Liu, X.M.; Li, L.; Jiang, Zhaoyuan; Zhang, J.H.; Guo, Xinrui; Ren, Jie

doi:10.1016/j.cropro.2012.11.020

Cited by 33 publications

(22 citation statements)

References 19 publications

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“…The combination of well managed cultural practices, fungicides, and resistant varieties are considered to be potential remedies for rice blast disease (Wang et al 2013). However, resistant varieties are considered to be the most important component and the most economically viable management strategy (Roychowdhury et al 2012).…”

Section: Response To Biotic Stressesmentioning

confidence: 99%

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

Rumanti¹,

Nugraha²,

Wening³

et al. 2016

Plant Breed. Biotech.

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Productivity of swampy land rice varieties in Indonesia remains low due to abiotic and biotic stresses. Iron toxicity, submergence and long-term flooding are common problems that affect plant growth, sometimes leading to total crop loss. Meanwhile, rice blast, rice tungro virus (RTV), and bacterial leaf blight (BLB) can also lead to severe yield loss. These stresses could be overcome by an integrative approach of cultural practices and planting multi-stress tolerant rice. Here we describe the development of new high-yielding varieties that are adapted to the swampy land environments in Indonesia with multi-stress tolerance to diverse abiotic and biotic stresses. Multi-environmental trials were performed in nine locations to screen for several abiotic and biotic stresses in the field and greenhouse in 2012 and 2013. This study identified promising rice lines that had high grain yield as well as tolerance to iron toxicity and submergence, resistance to BLB and RTV, and good grain quality. The best line was B11377F-MR-34-2, derived from a double cross among Cinglonik, IRBB7, Mamberamo and IR64. It was confirmed that this advanced breeding line carries the submergence tolerance gene, SUB1, through expression analysis of the SUB1A gene. Another promising line was B11586F-MR-11-2-2, having iron toxicity tolerance, resistance to RTV, and high milling recovery. Moreover, these lines have passed the Indonesian national variety release committee and have been named as Inpara8 and Inpara9, and are targeted for dissemination and adoption in the swampy areas.

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Section: Response To Biotic Stressesmentioning

confidence: 99%

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

Rumanti¹,

Nugraha²,

Wening³

et al. 2016

Plant Breed. Biotech.

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“…The Chinese rice genotype ''Lijiangxintuanheigu (LTH)'', a japonica rice variety, is highly susceptible to blast in various rice growing regions of the world (Le et al 2010;Wang et al 2013). On this premise LTH has been chosen as a universal susceptible background for the development of international monogenic blast differentials by the JIRCAS (Fukuta et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Genetic and physical mapping of a new allele of Pik locus from japonica rice ‘Liziangxintuanheigu’

et al. 2015

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The Chinese rice ''Liziangxintuanheigu (LTH)'' has been used as a universal susceptible background for the development of international monogenic blast differentials by the JIRCAS-IRRI network. Few reports on the occurrence of LTH incompatible blast isolates from the Philippines and India indicate that the genotype harbours unknown blast resistance gene(s). We report identification, mapping and physical delimitation of the chromosomal location of a new blast resistance gene from LTH. Preliminary linkage analysis of an F 2 mapping population generated from a cross between a susceptible cv. 'Dular' and LTH localized the blast resistance gene between simple sequence repeat (SSR) markers RM224 and RM6293 on the distal end of the long arm of chromosome 11. Further mapping with polymorphic SSR and sequence tagged site (STS) markers developed from the interval RM224-RM6293 delimited the resistance gene to a 2 cM interval flanked by STS markers STS-7 and STS-13. By aligning the sequences of linked markers on the sequence of cv. Nipponbare, a *168.05 kb region at the telomeric end of long of chromosome 11 was delineated as the region of the blast resistance gene. Six putatively expressed NBS-LRR genes were identified in the target region by surveying the equivalent genomic region of Nipponbare and two of these, LOC_Os11g46200 and LOC_Os11g46210, were short-listed as a potential candidate for the resistance gene. The new blast resistance gene designated as Pik-l was inferred to be a new allele of Pik locus based on its genomic position and distinct resistance spectra compared to previously known Pik alleles.

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“…Resistance conferred by Pi2 gene over time and space is evident and proved to be the most useful gene for durable resistance (Liu et al2002). Pi5 is associated with broad-spectrum resistance to diverse population of M. oryzae, being used in rice breeding programme in several countries (Wang et al 2013). Earlier, efforts were made to improve BPT5204 through MAS approach for bacterial leaf blight (BLB) resistance by pyramiding Xa21, xa13 and xa5 genes and for commercial cultivation (Sundaram et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Resistance conferred by Pi2 proved it to be an extreme useful gene in rice blast resistance programmes (Chen et al 1995;Liu et al 2002;Gouda et al 2012;). Pi5 gene on chromosome-9 encode proteins carrying three motifs characteristic of R genes: an Nterminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain and a leucine-rich repeat (LRR) motif associated with broad-spectrum resistance to diverse blast isolates (Jeon et al 2003;Wang et al 2013;Kishor et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Introgression of Pi2 and Pi5 Genes for Blast (Magnaporthe oryzae) Resistance in Rice and Field Evaluation of Introgression Lines for Resistance and Yield Traits

KrishnaMurthy

Deshmukh

Kumar

et al. 2017

Journal of Phytopathology

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Blast caused by Magnaporthe oryzae is the most devastating disease causing significant loss in rice production. The destructive nature of the disease is mainly due to the genetic plasticity of M. oryzae which complicates the breeding strategies. Blast can be effectively managed by the deployment of R genes. In this study, broad‐spectrum blast resistance genes Pi2 and Pi5 were introgressed independently into popular but blast susceptible rice variety, Samba Mahsuri (BPT5204) by applying marker‐assisted backcross breeding approach. Tightly linked markers AP5930 for Pi2 and 40N23r for Pi5 gene were used in foreground selection. Background selection helped to identify the lines with maximum recovery of recurrent parent genome (RPG). The RPG recovery in Pi2 introgression lines was up to 90.17 and 91.46% in Pi5 lines. Homozygous introgression lines in BC3F4 generation carrying Pi2 and Pi5 gene were field evaluated for blast resistance, yield per se and yield‐related traits. The lines showed resistance to leaf and neck blast in multilocation field evaluation. Improved BPT5204 lines with improvement for blast resistance were on par with original BPT5204 in terms of grain yield and grain features.

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Identification of rice blast resistance genes using international monogenic differentials

Cited by 33 publications

References 19 publications

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

Genetic and physical mapping of a new allele of Pik locus from japonica rice ‘Liziangxintuanheigu’

Introgression of Pi2 and Pi5 Genes for Blast (Magnaporthe oryzae) Resistance in Rice and Field Evaluation of Introgression Lines for Resistance and Yield Traits

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