Identification of ribosomal protein family in triple-negative breast cancer by bioinformatics analysis

Abstract

Triple-negative breast cancer (TNBC) accounts for ∼20% of all breast cancer (BC) cases. The management of TNBC represents a challenge due to its worse prognosis, heterogeneity and lack of targeted therapy. Moreover, its mechanisms are not fully clear. The aim of the study is to identify crucial genes between TNBC and non-TNBC for underlying targets for diagnostic and therapeutic methods of TNBC. The differentially expressed genes (DEGs) between TNBC and non-TNBC were selected from the Gene Expression Omnibus (GEO) database after the integrated analysis of two datasets (GSE65194 and GSE76124). Then Gene ontology (GO) and KEGG analysis were performed by DAVID database, protein–protein interaction (PPI) of DEGs was constructed by Search Tool for the Retrieval of Reciprocity Genes (STRING) database. Furthermore, centrality analysis and module analysis were carried out by Cytoscape to analyze the TNBC-related PPI. Subsequently, overall survival (OS) analysis was performed by GEPIA. Finally, the expressions of these key genes in TNBC and non-TNBC tissues were tested by qRT-PCR. The results showed that 955 DEGs were obtained, which were mainly enriched in ribosome, ribosomal subunit, and so on. Moreover, 19 candidate genes were focused on by centrality analysis and module analysis. Furthermore, we found the low expressions of ribosomal protein S9 (RPS9), ribosomal protein S14 (RPS14), ribosomal protein S27 (RPS27), ribosomal protein L11 (RPL11) and ribosomal protein L14 (RPL14) were related to a poor OS in BC patients. Additionally, qRT-PCR results suggested that these five genes were notably down-regulated in TNBC tissues. In summary, the present study suggests that ribosomal proteins are related to TNBC, and they may play an important role in the diagnosis, treatment and prognosis of TNBC.

Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma

“…Sun et al reported that RPL14(eL14) can rescue the progression role of miR-129-5p in cervical cancer cells [ 15 ]. In triple-negative breast cancer patients, the reduced expression of RPL14(eL14) was associated with lower survival rates [ 16 ]. Thus, literatures suggest that RPL14(eL14) is related to tumorigenesis.…”

Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats

“…3A ). Interestingly, low expression of ribosome-regulating genes including RPL11 was associated with a poor overall survival in triple-negative breast cancer patients [ 36 ]. Therefore, further work is necessary to determine the role of RPL11 in breast cancer and the consequences of its upregulation in anticancer effect of DATS.…”

The Gene Expression Analysis of Peripheral Blood Monocytes From Psoriasis Vulgaris Patients With Different Traditional Chinese Medicine Syndromes

“…At present, lots of studies on ribosomal proteins are related to cancer, and there is no study related to psoriasis. Ribosomal protein dysfunction is related to cancer, metabolic disorder and inflammation and ribosomal protein ubiquitination is a feedback mechanism of the body ( Wang W. et al, 2015 ; Lin et al, 2021 ). Our results show that the level of ubiquitinase decreases in psoriasis blood dryness syndrome, while ribosomal protein decreases in blood stasis syndrome, which may suggest that there are some connections and differences between the two syndrome types in inflammation and metabolism.…”