Identification of Resistance of Guangxi Oryza Rufipogon to Sogatella Furcifera with Stem Evaluation Method

Abstract

White-backed planthopper (WBPH) is a devastating pest of rice, which significantly reduces production and causes millions of dollars in losses worldwide annually. Screening of germplasm resources with resistance to white-backed planthopper plays a crucial role in crop insect resistance breeding. In the present research, a total of 1080 accessions of Guangxi common wild rice(Oryza rufipogon) was screened for WBPH resistance through stem evaluation method (SEM). After preliminary screening, 49 resistance resources were identified, accounting for 4.54% of the total. After verified identification at the adult stage, 15 resistance resources with stable resistance to white-backed planthopper were finally obtained as resistant resources. The regional distribution analysis showed that the highest frequency of WBPH resistance resources appeared in Fusui county located in the upper reaches of Xijiang river(6.80%). Meanwhile, the highest frequency of the high resistance (HR and R) resources appeared in Pingnan located in lower reaches(2.79%). The 49 resistant accessions were genotyped for 43 SSR markers evenly distributed on the 12 chromosomes. The genetic diversity analysis showed a high polymorphism level of common wild rice populations. Fusui county possesses higher level of genetic diversity than the Yongning district and Pingnan county populations. The results from this study are essential to analyze resistance genes, cultivate insect-resistant varieties, and provide regional guidance for the exploration of resistance resources in the future.