Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes

Saito, Shunya; Hoshi, Naomi; Zulkifli, Lalu; Widyastuti, Sri; Goshima, Shinobu; Drèyer, Ingo; Uozumi, Nobuyuki

doi:10.1080/19336950.2017.1372066

Cited by 2 publications

(2 citation statements)

References 33 publications

(35 reference statements)

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“…Loading of sucrose from the phloem to the apoplast requires activity of the shaker K + channel AKT2 (Shabala, 2003;Dreyer et al, 2017;Ragel et al, 2019). AKT2, usually weakly-rectified, can be c o n v e r t e d i n t o a n o n -r e c t i f y i n g K + c h a n n e l v i a phosphorylation, thereby enabling K + efflux and phloem membrane repolarization and the consequent retrieval of sucrose (Deeken et al, 2002;Michard et al, 2005a;Michard et al, 2005b;Gajdanowicz et al, 2011;Sandmann et al, 2011;Saito et al, 2017). Though the kinase responsible for this phosphorylation remains to be identified, it must be noted that AKT2 activity can be enhanced by the CBL4-CIPK6 complex in a Ca 2+ -dependent but phosphorylation-independent manner (Held et al, 2011).…”

Section: Possible Role Of Calcium In Balancing Of Energy Sourcementioning

confidence: 99%

Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients

Saito

Uozumi

2020

Front. Plant Sci.

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Essential elements taken up from the soil and distributed throughout the whole plant play diverse roles in different tissues. Cations and anions contribute to maintenance of intracellular osmolarity and the formation of membrane potential, while nitrate, ammonium, and sulfate are incorporated into amino acids and other organic compounds. In contrast to these ion species, calcium concentrations are usually kept low in the cytosol and calcium displays unique behavior as a cytosolic signaling molecule. Various environmental stresses stimulate increases in the cytosolic calcium concentration, leading to activation of calcium-regulated protein kinases and downstream signaling pathways. In this review, we summarize the stress responsive regulation of nutrient uptake and balancing by two types of calcium-regulated phosphorylation systems: CPK and CBL-CIPK. CPK is a family of protein kinases activated by calcium. CBL is a group of calcium sensor proteins that interact with CIPK kinases, which phosphorylate their downstream targets. In Arabidopsis, quite a few ion transport systems are regulated by CPKs or CBL-CIPK complexes, including channels/transporters that mediate transport of potassium (

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Section: Possible Role Of Calcium In Balancing Of Energy Sourcementioning

confidence: 99%

Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients

Saito

Uozumi

2020

Front. Plant Sci.

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“…Further characterization of the key KAT1 structural components has been carried out by random mutagenesis and complementation of the yeast trk1 trk2 mutant. These studies helped to define the positions in the pore that are determinant for K + selectivity [72,73,74,75,76]. KAT1 isoforms from other plant species, such as potato [21], maize [77] and rice [78] have also been identified and functionally characterized.…”

Section: Milestones In the Identification Of K+ Channels And Transmentioning

confidence: 99%

Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters

Locascio

Andrés-Colás

Mulet

et al. 2019

IJMS

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Sodium and potassium are two alkali cations abundant in the biosphere. Potassium is essential for plants and its concentration must be maintained at approximately 150 mM in the plant cell cytoplasm including under circumstances where its concentration is much lower in soil. On the other hand, sodium must be extruded from the plant or accumulated either in the vacuole or in specific plant structures. Maintaining a high intracellular K+/Na+ ratio under adverse environmental conditions or in the presence of salt is essential to maintain cellular homeostasis and to avoid toxicity. The baker’s yeast, Saccharomyces cerevisiae, has been used to identify and characterize participants in potassium and sodium homeostasis in plants for many years. Its utility resides in the fact that the electric gradient across the membrane and the vacuoles is similar to plants. Most plant proteins can be expressed in yeast and are functional in this unicellular model system, which allows for productive structure-function studies for ion transporting proteins. Moreover, yeast can also be used as a high-throughput platform for the identification of genes that confer stress tolerance and for the study of protein–protein interactions. In this review, we summarize advances regarding potassium and sodium transport that have been discovered using the yeast model system, the state-of-the-art of the available techniques and the future directions and opportunities in this field.

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Identification of regions responsible for the function of the plant K⁺ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes

Cited by 2 publications

References 33 publications

Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients

Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients

Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters

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