“…These miRNAs were found to be stably expressed with low variability for all the samples investigated (e.g., RPTECs and exosomes and urine samples from HC group) (Table S1); in addition, these miRNAs have previously been reported to be suitable reference genes for miRNA quantification. [41][42][43][44] Hsa-miR-26a-5p, hsa-miR-92a-3p, and hsa-miR-103a-3p were also reported to serve as suitable reference genes for human renal tissue and RPTECs under commonly applied conditions (e.g., basal conditions and stimulation with glucose, TNF-a, and TGF-b1). 45 Following normalization, we analyzed the delta Ct (DCt) values of exosomal miRNAs present in each group and determined that 30% of the 368 miRNAs analyzed were present in RPTEC exosome samples, whereas 34% were identified in HC samples.…”