Identification of recombinant AtPYL2, an abscisic acid receptor, in E. coli using a substrate-derived bioactive small molecule, a biotin linker with alkyne and amino groups, and a protein cross-linker

Anabuki, Tomoaki; Ito, Yusuke; Ohashi, Keisuke; Takasuka, Taichi E.; Matsuura, Hideyuki; Takahashi, Kosaku

doi:10.1016/j.bmcl.2019.126634

Cited by 1 publication

(3 citation statements)

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“…Previous report showed that our developed method was effective for isolating recombinant AtPYL2, a receptor of ABA [ 7 ]. As far as we know, the binding of ABA with AtTrxh3 has not yet been reported.…”

Section: Resultsmentioning

confidence: 99%

“…The eluted proteins were analyzed by peptide mass fingerprinting. The method described above succeeded in identifying a recombinant AtPYL2 from a protein extract of E. coli that overexpressed AtPYL2 [ 7 ].…”

Section: Figurementioning

confidence: 99%

“…We have developed new methods for chemical labeling and identification of a protein targeted by a bioactive small molecule [ 6 , 7 ]. The outline of the methods is depicted in Figure 2 .…”

Section: Introductionmentioning

confidence: 99%

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AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in Arabidopsis thaliana

et al. 2021

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Abscisic acid (ABA, 1) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of Arabidopsis thaliana. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%