Identification of rare traditional grapevine cultivars using SSR markers and their geographical location within the Czech Republic

Abstract

The designation of traditional varieties of grapevine is usually based on verbal information or very dated records. Old rare cultivars found in the Czech Republic were identified by Simple Sequence Repeat (SSR) analysis, a generally accepted method for cultivar identification. These cultivars are primarily maintained in a national collection of genetic resources. Finally a total of 102 candidate genotypes was selected where 49 traditional varieties were identified on the base of nine SSR loci compared with the European Vitis Database. Thirty-six items were registered under the correct designation. The remaining genotypes included four clones, and two genotypes could be described as synonyms. Seven genotypes were found to be incorrectly marked. For three of them, the correct name was found in the database under their SSR profile and four items were considered to be unique as no identical profile was found.

Comparison of DNA methylation landscape between Czech and Armenian vineyards show their unique character and increased diversity

“…Cultivar authenticity of all samples was confirmed by SSR analysis of 9 microsatellite loci used worldwide for cultivar identification purposes (This et al 2004). SSR analysis was performed according to protocol (Baránková et al 2020).…”

“…The main advantage of SSR markers is that there exists a worldwide accessible database of SSR fingerprints covering thousands of grapevine cultivars (The European Vitis Database). Moreover, selected SSR markers are respected by International Organisation of Vine and Wine (OIV) as official descriptors frequently used for effective cultivar identification (Baránková et al 2020).…”

DNA-Based Technologies for Grapevine Biodiversity Exploitation: State of the Art and Future Perspectives

“…The genome of grape varieties is marked by different SSR patterns that have been used to reveal the pedigree of several varieties [54,55] and their historical origins [12,13,16,55,56] since the 1990s. SSRs have revealed unexpected synonyms [57][58][59] also in grape varieties from distant geographical areas, as in the case of Malvasia delle Lipari, Malvasia di Sardegna, Greco di Gerace (Italy), Malvasia de Sitges (Spain) and Malvasia dubrovačka (Croatia) [60]. Owing to their extensive use worldwide, large international Vitis databases containing SSR profiles are now available as references for cultivar identification (http://www.eu-vitis.de/index.php (accessed on 23 January 2022); http://www.vivc.de (accessed on 23 January 2022)).…”

“…Owing to their extensive use worldwide, large international Vitis databases containing SSR profiles are now available as references for cultivar identification (http://www.eu-vitis.de/index.php (accessed on 23 January 2022); http://www.vivc.de (accessed on 23 January 2022)). To standardize the molecular analysis protocols, a set of nine SSRs has been established as a reference kit for grapevine genotyping [58,59] and several public Vitis databases have been established, including the European [61], Greek [62], Swiss [63] and Italian [64] Vitis databases. Interestingly, Kim et al [65] developed a set of 21 SSR markers suitable for amplifying both vinifera and non-vinifera species and, therefore, universal candidate markers for cross-species genotyping.…”