Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L.

Ávila, Carmen M.; Sillero, Josefina C.; Rubiales, Diego; Moreno, M. T.; Torres, A. M.

doi:10.1007/s00122-003-1254-8

Cited by 77 publications

(33 citation statements)

References 33 publications

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“…The simultaneous use of both types of markers allows their use as a codominant marker. Different genes might be available in the different accessions displaying hypersensitivity reported so far (Sillero et al 2000;Sillero and Rubiales 2002;Rubiales and Sillero 2003), but genetic analysis has so far been performed only with one of them (Avila et al 2003). Obviously, this preliminary work ought to be complemented with the identification of the different genes and their associated markers before an efficient pyramidization programme can be achieved.…”

Section: Rusts Of Legume Cropsmentioning

confidence: 93%

“…It is controlled by genes with major effects (Sillero et al 2000). Use of bulk segregant analysis (BSA) allowed identifying three random amplified polymorphic DNA (RAPD) markers linked to resistance gene (Uvf-1) (Avila et al 2003). Three RAPD markers (OPD13 736 , OPL18 1032 and OPI20 900 ) were mapped in coupling phase to the resistance gene Uvf-1.…”

Section: Rusts Of Legume Cropsmentioning

confidence: 99%

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Legume breeding for rust resistance: lessons to learn from the model Medicago truncatula

et al. 2011

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Rusts are major biotic constraints of legumes worldwide. Breeding for rust resistance is regarded as the most cost efficient method for rust control. However, in contrast to common bean for which complete monogenic resistance exists and is efficiently used, most of the rust resistance reactions described so far in cool season food legumes are incomplete and of complex inheritance. Incomplete resistance has been described in faba bean, pea, chickpea and lentil and several of their associated QTLs have been mapped. However, the relatively large distance between the QTLs and their associated molecular markers hampers their efficient use for marker assisted selection. Their large genome size drastically hampers the development of genomic resource and limits the saturation of their genetic maps. The use of model plants such as the model legume Medicago truncatula may circumvent this drawback. The important genetic and genomic resources and tools available for this model legume can considerably speed up the discovery and validation of new genes and QTLs in resistance to legume pathogens. Here, the potential of M. truncatula as a model to study rust resistance in legumes, and to transfer rust resistance genes to cool season grain legumes is reviewed.

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Section: Rusts Of Legume Cropsmentioning

confidence: 93%

Section: Rusts Of Legume Cropsmentioning

confidence: 99%

Legume breeding for rust resistance: lessons to learn from the model Medicago truncatula

et al. 2011

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“…Moreover, the first attempts to map genes and quantitative trait loci (QTLs) for resistance to parasitic plants (O. crenata) and fungal diseases (A. fabae and U. viciae-fabae) have been recently reported (Román et al, , 2003Avila et al, 2003Avila et al, , 2004.…”

Section: Genetic and Genomic Resourcesmentioning

confidence: 99%

Faba bean breeding for resistance against biotic stresses: Towards application of marker technology

et al. 2006

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Faba beans are adversely affected by numerous fungal diseases leading to a steady reduction in the cultivated area in many countries. Major diseases such as Ascochyta blight (Ascochyta fabae), rust (Uromyces viciae-fabae), chocolate spot (Botrytis fabae), downy mildew (Peornospora viciae) and foot rots (Fusarium spp.) are considered to be the major constraints to the crop. Importantly, broomrape (Orobanche crenata), a very aggressive parasitic angiosperm, is the most damaging and widespread enemy along the Mediterranean basin and Northern Africa. Recent mapping studies have allowed the identification of genes and QTLs controlling resistance to some of these diseases. In case of broomrape, 3 QTLs explained more than 70% of the phenotypic variance of the trait. Concerning Ascochyta, two QTLs located in chromosomes 2 and 3 explained 45% of variation. A second population sharing the susceptible parental line also revealed two QTLs, one of them likely sharing chromosomal location and jointly contributing with a similar percentage of the total phenotypic variance. Finally, several RAPD markers linked to a gene determining hypersensitive resistance to race 1 of the rust fungus U. viciae-fabae have also been reported. The aim of this paper is to review the state of the art of gene technology for genetic improvement of faba bean against several important biotic stresses. Special emphasis is given on the application of marker technology, and Quantitative Trait Loci (QTL) analysis for Marker-Assisted Selection (MAS) in the species. Finally, the potential use of genomic tools to facilitate breeding in the species is discussed. The combined approach should expedite the future development of lines and cultivars with multiple disease resistance, one of the top priorities in faba bean research programs.

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“…Over the past years, molecular markers have been identified for genes/QTLs controlling resistance to several biotic stresses in legumes, such as soybean (Kim and Diers 2000;Arahana et al 2001;Li et al 2002;Yuan et al 2002), bean (Miklas et al 2001;Park et al 2001;Schneider et al 2001;Fall et al 2002), lentil (Ford et al 1999), chickpea (Santra et al 2000) or faba bean (Roman et al , 2003Avila et al 2003b). The identification of molecular markers tightly linked to the A. fabae resistance genes may provide a valuable alternative to the time-consuming disease evaluation.…”

Section: Introductionmentioning

confidence: 99%

Isolate and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L).

Ávila¹,

Šatović

Sillero³

et al. 2003

Theor Appl Genet

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The main objective of the present study was to locate the genomic regions responsible for ascochyta blight resistance in faba bean. Six QTLs were identified with the help of a linkage map constructed from a F(2) population from the cross between the inbred lines 29H (resistant) and VF136 (susceptible). Two pathogenically distinct Ascochyta isolates were used to study the genetic control against them and disease evaluations were performed separately on leaves and stems to investigate whether different genetic systems control resistance in each plant organ, as previously suggested. The six QTLs detected were named Af3 to Af8. Af3 and Af4 were effective against both Ascochyta isolates, Af5 was only effective against isolate CO99-01 while Af6, Af7 and Af8 were only effective against isolate LO98-01. Af3, Af4, Af5 and Af7 were revealed in both leaves and stems. By contrast, Af6 was only effective in leaves and Af8 only in stems. The validity and application of these results in a MAS program is discussed.

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Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L.

Cited by 77 publications

References 33 publications

Legume breeding for rust resistance: lessons to learn from the model Medicago truncatula

Legume breeding for rust resistance: lessons to learn from the model Medicago truncatula

Faba bean breeding for resistance against biotic stresses: Towards application of marker technology

Isolate and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L).

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