Identification of RAPD markers closely linked to the mlo‐locus in barley

Abstract

Developing resistance to powdery mildew, Erysiphe graminis f.sp. hordei, is a major goal of many barley breeding programmes. Several resistance genes have been tagged or mapped with molecular markers. The mlo gene confers durable resistance towards all known isolates of the pathogen. In this study, RAPD markers and bulked segregant analysis were used to determine PCR-based markers linked to the mlolocus. Sixty doubled haploid hnes from a cross between an isogenic line of'Ingrid' carrying the mloW allele and a susceptible cv. 'Pokko' were used as plant material. Seven linked RAPD markers were found, the closest lying 1.6 cM away from the resistance gene. When eight barley varieties were assayed for the presence of this band, F4-980, it was found in the resistant varieties but not in the susceptible ones. The linked marker bands could be amplified from DNA-samples prepared by using three different methods, including a quick squash technique. PCR-based markers hnked to the resistance gene can be used as tools for selection in breeding programmes.

DNA polymorphisms among near-isogenic lines for powdery-mildew-resistant genes in barley detected by primers targeting resistance-gene analogs

“…These newly de- scribed markers are potentially valuable tools for breeders to pyramid genes conferring resistance to E. graminis f. sp. hordei (Manninen et al 1997). The use of marker-assisted selection technique in plant breeding can increase selection precision and efficiency (Lee 1995;Knapp 1998).…”

Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding

“…Czembor and Talbert (1997) and Schwarz et al (1999) described the utilization of RFLP (Restriction Fragment Length Polymorphism) and AFLP (Amplified Fragment Length Polymorphism) markers, respectively, for the Mla locus determining the resistance to Blumeria graminis. Manninen et al (1997) identified an RAPD (Random Amplified Polymorphic Region) marker closely linked to the mlo locus, which also confers the effective resistance to the same pathogen. Molecular and classical breeding techniques were employed to develop cultivars with resistance to five different fungal diseases in the Minnesota Barley Improvement Program (Steffenson & Smith 2006).…”