Identification of Random Amplified Polymorphic DNA Markers Linked to the Co-4 Resistance Gene to Colletotrichum lindemuthianum in Common Bean

Abstract

New cultivars of the common bean (Phaseolus vulgaris) with durable resistance to anthracnose can be developed by pyramiding major resistance genes using marker-assisted selection. To this end, it is necessary to identify sources of resistance and molecular markers tightly linked to the resistance genes. The objectives of this work were to study the inheritance of resistance to anthracnose in the cultivar TO (carrying the Co-4 gene), to identify random amplified polymorphic DNA (RAPD) markers linked to Co-4, and to introgress this gene in the cultivar Rudá. Populations F(1), F(2), F(2:3), BC(1)s, and BC(1)r from the cross Rudá x TO were inoculated with race 65 of Colletotrichum lindemuthianum, causal agent of bean anthracnose. The phenotypic ratios (resistant/susceptible) were 3:1 in the F(2) population, 1:1 in the BC(1)s, and 1:0 in the BC(1)r, confirming that resistance to anthracnose in the cultivar TO was monogenic and dominant. Six RAPD markers linked to the Co-4 gene were identified, four in the coupling phase: OPY20(830C) (0.0 centimorgan [cM]), OPC08(900C) (9.7 cM), OPI16(850C) (14.3 cM), and OPJ01(1,380C) (18.1 cM); and two in the repulsion phase: OPB03(1,800T) (3.7 cM) and OPA18(830T) (17.4 cM). OPY20(830C) and OPB03(1,800T), used in association as a codominant pair, allowed the identification of the three genotypic classes with a high degree of confidence. Marker OPY20(830C), which is tightly linked to Co-4, is being used to assist in breeding for resistance to anthracnose.

Association between RAPD marker OPAS13950C and anthracnose resistance allele Co-43 of common bean

“…These primers amplify two molecular markers that have been identified as linked to Co-4 of cultivar TO (OPY20 830C ) and to Co-4 2 (OPAS13 950C ) of cultivars G2333 and Selection 1308 (Arruda et al 2000, Alzate-Marin et al 2001b, Young et al 1998. DNA amplification of PI 207262 with these molecular markers linked to Co-4 reinforces the finding that this cultivar carries an allele of this gene.…”

“…Co-4 gene is present in cultivar TO and cultivar G2333 and Selection 1308 (derived from G 2333) owns the Co-4 2 allele. The molecular markers OPY20 830C and OPAS13 950C were identified as linked at 0.0 cM from Co-4 gene of TO and Co-4 2 allele of Selection 1308 (Arruda et al 2000, Young et al 1998. In a population derived from cultivar G2333, OPAS13 950C was located at 5.6 cM from Co-4 2 allele (Alzate- Marin et al 2001b).…”

Introgression of resistance to pathogens in common bean lines with the aid of molecular markers

“…To check for the possibility of use of molecular marker assisted selection in the populations, ten plants from each one of the parents were genotyped, with the markers SCARY20 830a (Arruda et al 2000), SCARF10 1050a (Correa et al 2000) and SCARH13 520a (Queiroz et al 2004). …”

Breeding of Common Bean for Resistance to the Physiological Races of Anthracnose Identified in Bulgaria

“…currently, races of C. lindemuthianum are classified by inoculating isolates onto a universal set of 12 differential cultivars and named by a binary nomenclature system (36). high genetic variability for C. lindemuthianum has been described worldwide, and more than 78 different races of the pathogen have been identified (3,4,5,10,12,15). The first investigations on the pathogenic variability of C. lindemuthianum in Bulgaria were carried out by Genchev, who identified two physiological races: 81 and 130 (19,21).…”

“…An efficient RAPD marker for identification of Co-1 gene is oF10 530 (46); for Co-2 gene are oq4 1440 , oh20 450 and B355 1000 (1,45); and for Co-4 gene are oPY20 830c , oPB03 180t , SAS13 and Sh18 (12,47). A successful ScAR markers for Co-2 gene are Sch20 450 codominant , ScF3 dominant , ScAreoli 1000 (1,2,34) and for Co-4 gene are SY20 830c and Sq4 (7,14).…”