2006
DOI: 10.1128/jb.188.7.2554-2567.2006
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Identification of RamA, a Novel LuxR-Type Transcriptional Regulator of Genes Involved in Acetate Metabolism of Corynebacterium glutamicum

Abstract: In Corynebacterium glutamicum, the acetate-activating enzymes phosphotransacetylase and acetate kinase and the glyoxylate cycle enzymes isocitrate lyase and malate synthase are coordinately up-regulated in the presence of acetate in the growth medium. This regulation is due to transcriptional control of the respective ptaack operon and the aceA and aceB genes, brought about at least partly by the action of the negative transcriptional regulator RamB. Using cell extracts of C. glutamicum and employing DNA affin… Show more

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Cited by 105 publications
(156 citation statements)
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“…Moreover, expression of the representative RamA regulon members sugR and aceA in strain KT27 was also comparable to that in the wild type (data not shown). As RamA is essential for expression of aceA encoding isocitrate lyase in the glyoxylate shunt, the ramA mutant shows a growth defect on acetate (38). In contrast, the growth of strain KT27 on acetate was comparable to that of the wild type (data not shown).…”
Section: Resultsmentioning
confidence: 72%
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“…Moreover, expression of the representative RamA regulon members sugR and aceA in strain KT27 was also comparable to that in the wild type (data not shown). As RamA is essential for expression of aceA encoding isocitrate lyase in the glyoxylate shunt, the ramA mutant shows a growth defect on acetate (38). In contrast, the growth of strain KT27 on acetate was comparable to that of the wild type (data not shown).…”
Section: Resultsmentioning
confidence: 72%
“…The ␤-galactosidase activity driven by the native and mutated promoters during growth in nutrient-rich medium (A medium) containing either 1% glucose or 1% acetate was determined. We used the medium because the ramA deletion mutant strains described in the following section are incapable of growing on acetate as the sole carbon source, as has been reported (38). The activities were largely unchanged during growth, although they slightly increased in some of these strains used in the later growth phase.…”
Section: Resultsmentioning
confidence: 99%
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“…of cDNA ends] method, generation of polyclonal antibodies, and Western blotting) were described previously (3,4,6).…”
mentioning
confidence: 99%